Protein S

PROS1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1Z6C
Identifiers
Aliases	PROS1 , PROS, PS21, PS22, PS23, PS24, PS25, PSA, THPH5, THPH6, protein S (alpha), protein S
External IDs	OMIM: 176880; MGI: 1095733; HomoloGene: 264; GeneCards: PROS1; OMA:PROS1 - orthologs
Gene location (Human) Chr. Chromosome 3 (human) [1] Band 3q11.1 Start 93,873,051 bp [1] End 93,980,003 bp [1]
Gene location (Mouse) Chr. Chromosome 16 (mouse) [2] Band 16|16 C1.3 Start 62,674,670 bp [2] End 62,749,709 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Epithelium of choroid plexus bronchial epithelial cell synovial joint germinal epithelium parietal pleura pericardium liver visceral pleura spinal ganglia trigeminal ganglion Top expressed in cumulus cell decidua stroma of bone marrow aortic valve endothelial cell of lymphatic vessel gastrula iris calvaria left lung lobe sciatic nerve More reference expression data BioGPS More reference expression data
Gene ontology Molecular function calcium ion binding endopeptidase inhibitor activity Cellular component blood microparticle endoplasmic reticulum membrane Golgi membrane plasma membrane extracellular region Golgi lumen extracellular exosome platelet alpha granule lumen extracellular space Biological process hemostasis fibrinolysis platelet degranulation blood coagulation endoplasmic reticulum to Golgi vesicle-mediated transport signal peptide processing peptidyl-glutamic acid carboxylation regulation of complement activation leukocyte migration negative regulation of endopeptidase activity negative regulation of blood coagulation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5627 19128 Ensembl ENSG00000184500 ENSMUSG00000022912 UniProt P07225 Q08761 RefSeq (mRNA) NM_000313 NM_001314077 NM_011173 RefSeq (protein) NP_000304 NP_001301006 NP_035303 Location (UCSC) Chr 3: 93.87 – 93.98 Mb Chr 16: 62.67 – 62.75 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Protein S (also known as PROS) is a vitamin K-dependent plasma glycoprotein synthesized in the liver. In the circulation, Protein S exists in two forms: a free form and a complex form bound to complement protein C4b-binding protein (C4BP). In humans, protein S is encoded by the PROS1 gene. ^{[5] [6]} Protein S plays a role in coagulation.

History

Protein S is named for Seattle, Washington, where it was originally discovered and purified ^[7] by Earl Davie's group in 1977. ^[8]

Structure

Protein S is partly homologous to other vitamin K-dependent plasma coagulation proteins, such as protein C and factors VII, IX, and X. Similar to them, it has a Gla domain and several EGF-like domains (four rather than two), but no serine protease domain. Instead, there is a large C-terminus domain that is homologous to plasma steroid hormone-binding proteins such as sex hormone-binding globulin and corticosteroid-binding globulin. It may play a role in the protein functions as either a cofactor for activated protein C (APC) or in binding C4BP. ^{[9] [10]}

Additionally, protein S has a peptide between the Gla domain and the EGF-like domain, that is cleaved by thrombin. The Gla and EGF-like domains stay connected after the cleavage by a disulfide bond. However, protein S loses its function as an APC cofactor following either this cleavage or binding C4BP. ^[11]

Function

The best characterized function of Protein S is its role in the anti coagulation pathway, where it functions as a cofactor to Protein C in the inactivation of Factors Va and VIIIa. Only the free form has cofactor activity. ^[12]

Protein S binds to negatively charged phospholipids via the carboxylated Gla domain. This property allows Protein S to facilitate the removal of cells that are undergoing apoptosis, a form of structured cell death used by the body to remove unwanted or damaged cells. In healthy cells, an ATP (adenosine triphosphate)-dependent enzyme removes negatively charged phospholipids such as phosphatidyl serine from the outer leaflet of the cell membrane. An apoptotic cell (that is, one undergoing apoptosis) no longer actively manages the distribution of phospholipids in its outer membrane and hence begins to display negatively charged phospholipids on its exterior surface. These negatively charged phospholipids are recognized by phagocytes such as macrophages. Protein S binds to the negatively charged phospholipids and functions as a bridge between the apoptotic cell and the phagocyte. This bridging expedites phagocytosis and allows the cell to be removed without giving rise to inflammation or other signs of tissue damage.

Protein S does not bind to the nascent complement complex C5,6,7 to prevents it from inserting into a membrane. This is a different complement protein S AKA vitronectin made by the VTN gene, not to be confused with the coagulation protein S made by the PROS gene which this wiki page concerns.

Pathology

Mutations in the PROS1 gene can lead to Protein S deficiency which is a rare blood disorder which can lead to an increased risk of thrombosis. ^{[13] [14]} The SARS-CoV-2 papain-like protease (PLpro) was shown to cleave a sequence (LRGG*KIEVQL) in PROS1. ^[15] The cleavage of PROS1 may lead to a transient deficiency in PROS1 during or after infection and may be associated with COVID coagulopathy. ^[16]

Interactions

Protein S has been shown to interact with Factor V. ^{[17] [18]} A sequence in PROS1 can be cut by the papain-like protease of SARS-CoV-2. ^[15]

See also

• Hemostasis

References

1. GRCh38: Ensembl release 89: ENSG00000184500 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000022912 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Lundwall A, Dackowski W, Cohen E, Shaffer M, Mahr A, Dahlbäck B, et al. (September 1986). "Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation". Proceedings of the National Academy of Sciences of the United States of America. 83 (18): 6716–6720. Bibcode:1986PNAS...83.6716L. doi: 10.1073/pnas.83.18.6716 . PMC   386580 . PMID   2944113.
6. Long GL, Marshall A, Gardner JC, Naylor SL (January 1988). "Genes for human vitamin K-dependent plasma proteins C and S are located on chromosomes 2 and 3, respectively". Somatic Cell and Molecular Genetics. 14 (1): 93–98. doi:10.1007/BF01535052. PMID   2829367. S2CID   31236887.
7. Bauer KA (February 2025). Leung LL, Tirnauer JS (eds.). "Protein S deficiency". UpToDate. Retrieved May 10, 2017.
8. Kaushansky K, Lichtman M, Prchal J, Levi M, Press O, Burns L, et al. (2015). Williams Hematology. McGraw-Hill. p. 1926.
9. Stenflo J (1999). "Contributions of Gla and EGF-like domains to the function of vitamin K-dependent coagulation factors". Critical Reviews in Eukaryotic Gene Expression. 9 (1): 59–88. doi:10.1615/CritRevEukaryotGeneExpr.v9.i1.50. PMID   10200912.
10. Rosner W (December 1991). "Plasma steroid-binding proteins". Endocrinology and Metabolism Clinics of North America. 20 (4): 697–720. doi:10.1016/S0889-8529(18)30240-8. PMID   1778174.
11. Dahlbäck B, Lundwall A, Stenflo J (June 1986). "Primary structure of bovine vitamin K-dependent protein S". Proceedings of the National Academy of Sciences of the United States of America. 83 (12): 4199–4203. Bibcode:1986PNAS...83.4199D. doi: 10.1073/pnas.83.12.4199 . PMC   323699 . PMID   2940598.
12. Castoldi E, Hackeng TM (September 2008). "Regulation of coagulation by protein S". Current Opinion in Hematology. 15 (5): 529–536. doi:10.1097/MOH.0b013e328309ec97. PMID   18695379. S2CID   11522770.
13. Beauchamp NJ, Dykes AC, Parikh N, Campbell Tait R, Daly ME (June 2004). "The prevalence of, and molecular defects underlying, inherited protein S deficiency in the general population". British Journal of Haematology. 125 (5): 647–654. doi:10.1111/j.1365-2141.2004.04961.x. PMID   15147381. S2CID   705661.
14. García de Frutos P, Fuentes-Prior P, Hurtado B, Sala N (September 2007). "Molecular basis of protein S deficiency". Thrombosis and Haemostasis. 98 (3): 543–556. doi:10.1160/th07-03-0199. PMID   17849042. S2CID   17274778.
15. Reynolds ND, Aceves NM, Liu JL, Compton JR, Leary DH, Freitas BT, et al. (June 2021). "The SARS-CoV-2 SSHHPS Recognized by the Papain-like Protease". ACS Infectious Diseases. 7 (6): 1483–1502. doi:10.1021/acsinfecdis.0c00866. PMC   8171221 . PMID   34019767.
16. Baroni M, Beltrami S, Schiuma G, Ferraresi P, Rizzo S, Passaro A, et al. (February 2024). "In Situ Endothelial SARS-CoV-2 Presence and PROS1 Plasma Levels Alteration in SARS-CoV-2-Associated Coagulopathies". Life. 14 (2): 237. Bibcode:2024Life...14..237B. doi: 10.3390/life14020237 . PMC   10890393 . PMID   38398746.
17. Heeb MJ, Kojima Y, Rosing J, Tans G, Griffin JH (December 1999). "C-terminal residues 621-635 of protein S are essential for binding to factor Va". The Journal of Biological Chemistry. 274 (51): 36187–36192. doi: 10.1074/jbc.274.51.36187 . PMID   10593904. S2CID   45995946.
18. Heeb MJ, Mesters RM, Tans G, Rosing J, Griffin JH (February 1993). "Binding of protein S to factor Va associated with inhibition of prothrombinase that is independent of activated protein C". The Journal of Biological Chemistry. 268 (4): 2872–2877. doi: 10.1016/S0021-9258(18)53854-0 . PMID   8428962.

Further reading

• Dahlbäck B (July 1991). "Protein S and C4b-binding protein: components involved in the regulation of the protein C anticoagulant system". Thrombosis and Haemostasis. 66 (1): 49–61. doi:10.1055/s-0038-1646373. PMID   1833851. S2CID   24929072.
• Witt I (May 2002). "[Molecular biological basis and diagnosis of hereditary defect of antithrombin III, protein c and protein S]" [Molecular biological basis and diagnosis of hereditary defect of antithrombin III, protein c and protein S]. Hamostaseologie (in German). 22 (2): 14–24. doi:10.1055/s-0037-1619540. PMID   12193972. S2CID   58077740.
• Rezende SM, Simmonds RE, Lane DA (February 2004). "Coagulation, inflammation, and apoptosis: different roles for protein S and the protein S-C4b binding protein complex". Blood. 103 (4): 1192–1201. doi: 10.1182/blood-2003-05-1551 . PMID   12907438. S2CID   133028.
• Dahlbäck B (July 2007). "The tale of protein S and C4b-binding protein, a story of affection". Thrombosis and Haemostasis. 98 (1): 90–96. doi:10.1160/th07-04-0269. PMID   17597997. S2CID   18823655.
• García de Frutos P, Fuentes-Prior P, Hurtado B, Sala N (September 2007). "Molecular basis of protein S deficiency". Thrombosis and Haemostasis. 98 (3): 543–556. doi:10.1160/th07-03-0199. PMID   17849042. S2CID   17274778.
• Maillard C, Berruyer M, Serre CM, Dechavanne M, Delmas PD (March 1992). "Protein-S, a vitamin K-dependent protein, is a bone matrix component synthesized and secreted by osteoblasts". Endocrinology. 130 (3): 1599–1604. doi:10.1210/endo.130.3.1531628. PMID   1531628.
• Griffin JH, Gruber A, Fernández JA (June 1992). "Reevaluation of total, free, and bound protein S and C4b-binding protein levels in plasma anticoagulated with citrate or hirudin". Blood. 79 (12): 3203–3211. doi: 10.1182/blood.V79.12.3203.bloodjournal79123203 . PMID   1534488.
• Guglielmone HA, Vides MA (January 1992). "A novel functional assay of protein C in human plasma and its comparison with amidolytic and anticoagulant assays". Thrombosis and Haemostasis. 67 (1): 46–49. doi:10.1055/s-0038-1648377. PMID   1615482. S2CID   27769717.
• Bertina RM, Ploos van Amstel HK, van Wijngaarden A, Coenen J, Leemhuis MP, Deutz-Terlouw PP, et al. (August 1990). "Heerlen polymorphism of protein S, an immunologic polymorphism due to dimorphism of residue 460". Blood. 76 (3): 538–548. doi: 10.1182/blood.V76.3.538.538 . PMID   2143091.
• Schmidel DK, Tatro AV, Phelps LG, Tomczak JA, Long GL (August 1990). "Organization of the human protein S genes". Biochemistry. 29 (34): 7845–7852. doi:10.1021/bi00486a010. PMID   2148110.
• Ploos van Amstel HK, Reitsma PH, van der Logt CP, Bertina RM (August 1990). "Intron-exon organization of the active human protein S gene PS alpha and its pseudogene PS beta: duplication and silencing during primate evolution". Biochemistry. 29 (34): 7853–7861. doi:10.1021/bi00486a011. PMID   2148111.
• Allaart CF, Aronson DC, Ruys T, Rosendaal FR, van Bockel JH, Bertina RM, et al. (October 1990). "Hereditary protein S deficiency in young adults with arterial occlusive disease". Thrombosis and Haemostasis. 64 (2): 206–210. PMID   2148653.
• Ohlin AK, Landes G, Bourdon P, Oppenheimer C, Wydro R, Stenflo J (December 1988). "Beta-hydroxyaspartic acid in the first epidermal growth factor-like domain of protein C. Its role in Ca2+ binding and biological activity". The Journal of Biological Chemistry. 263 (35): 19240–19248. doi: 10.1016/S0021-9258(18)37415-5 . PMID   2461936.
• Schwarz HP, Heeb MJ, Lottenberg R, Roberts H, Griffin JH (July 1989). "Familial protein S deficiency with a variant protein S molecule in plasma and platelets". Blood. 74 (1): 213–221. doi: 10.1182/blood.V74.1.213.213 . PMID   2526663.
• Ploos van Amstel HK, van der Zanden AL, Reitsma PH, Bertina RM (September 1987). "Human protein S cDNA encodes Phe-16 and Tyr 222 in consensus sequences for the post-translational processing". FEBS Letters. 222 (1): 186–190. Bibcode:1987FEBSL.222..186V. doi: 10.1016/0014-5793(87)80217-X . PMID   2820795. S2CID   46365357.
• Dahlbäck B, Lundwall A, Stenflo J (June 1986). "Primary structure of bovine vitamin K-dependent protein S". Proceedings of the National Academy of Sciences of the United States of America. 83 (12): 4199–4203. Bibcode:1986PNAS...83.4199D. doi: 10.1073/pnas.83.12.4199 . PMC   323699 . PMID   2940598.
• Lundwall A, Dackowski W, Cohen E, Shaffer M, Mahr A, Dahlbäck B, et al. (September 1986). "Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation". Proceedings of the National Academy of Sciences of the United States of America. 83 (18): 6716–6720. Bibcode:1986PNAS...83.6716L. doi: 10.1073/pnas.83.18.6716 . PMC   386580 . PMID   2944113.
• Engesser L, Broekmans AW, Briët E, Brommer EJ, Bertina RM (May 1987). "Hereditary protein S deficiency: clinical manifestations". Annals of Internal Medicine. 106 (5): 677–682. doi:10.7326/0003-4819-106-5-677. PMID   2952034.
• Watkins PC, Eddy R, Fukushima Y, Byers MG, Cohen EH, Dackowski WR, et al. (January 1988). "The gene for protein S maps near the centromere of human chromosome 3". Blood. 71 (1): 238–241. doi:10.1182/blood.V71.1.238.238. PMID   2961379.