| Date | Name | Riding | Affiliation | Details |
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| January 23, 2006 | See List of Members | Election day of the 39th Canadian federal election |
| February 6, 2006 | David Emerson | Vancouver Kingsway | Conservative | Appointed to cabinet, crossed the floor from the Liberals |
| August 28, 2006 | Benoît Sauvageau | Repentigny | Bloc Québécois | Died in a car accident |
| September 20, 2006 | Joe Fontana | London North Centre | Liberal | Vacated seat to run for Mayor of London, Ontario |
| October 18, 2006 | Garth Turner | Halton | Independent | Removed from the Conservative caucus on after being accused of breaking caucus confidentiality. |
| November 27, 2006 | Raymond Gravel | Repentigny | Bloc Québécois | Elected in a by-election |
| November 27, 2006 | Glen Pearson | London North Centre | Liberal | Elected in a by-election |
| January 5, 2007 | Wajid Khan | Mississauga—Streetsville | Conservative | Crossed the floor from the Liberals |
| January 28, 2007 | Jean Lapierre | Outremont | Liberal | Vacated seat to pursue television career |
| February 6, 2007 | Garth Turner | Halton | Liberal | Joined the Liberal caucus |
| February 21, 2007 | Yvan Loubier | Saint-Hyacinthe—Bagot | Bloc Québécois | Vacated seat to run in the 2007 Quebec general election |
| March 21, 2007 | Joe Comuzzi | Thunder Bay—Superior North | Independent | Removed from the Liberal caucus on due to his intention to vote for the 2007 budget. |
| April 12, 2007 | Louise Thibault | Rimouski-Neigette—Témiscouata—Les Basques | Independent | Left the Bloc Québécois caucus |
| June 5, 2007 | Bill Casey | Cumberland—Colchester—Musquodoboit Valley | Independent | Expelled from the Conservative caucus for voting against his party's budget. |
| June 26, 2007 | Joe Comuzzi | Thunder Bay—Superior North | Conservative | Joined the Conservative caucus. |
| July 2, 2007 | Bill Graham | Toronto Centre | Liberal | Vacated seat |
| July 12, 2007 | Jim Peterson | Willowdale | Liberal | Vacated seat |
| July 27, 2007 | Stephen Owen | Vancouver Quadra | Liberal | Vacated seat |
| July 29, 2007 | Michel Gauthier | Roberval—Lac-Saint-Jean | Bloc Québécois | Vacated seat |
| August 31, 2007 | Gary Merasty | Desnethé—Missinippi—Churchill River | Liberal | Vacated seat |
| September 17, 2007 | Denis Lebel | Roberval—Lac-Saint-Jean | Conservative | Elected in a by-election |
| September 17, 2007 | Thomas Mulcair | Outremont | New Democratic | Elected in a by-election |
| September 17, 2007 | Ève-Mary Thaï Thi Lac | Saint-Hyacinthe—Bagot | Bloc Québécois | Elected in a by-election |
| October 28, 2007 | Blair Wilson | West Vancouver—Sunshine Coast—Sea to Sky Country | Liberal without caucus | Removed from the Liberal caucus, still identified as a Liberal |
| November 23, 2007 | Wajid Khan | Mississauga—Streetsville | Independent | Left the Conservative caucus to sit as an independent |
| January 25, 2008 | Lucienne Robillard | Westmount—Ville-Marie | Liberal | Vacated seat |
| January 29, 2008 | Blair Wilson | West Vancouver—Sunshine Coast—Sea to Sky Country | Independent | Officially became an Independent |
| February 4, 2008 | Wajid Khan | Mississauga—Streetsville | Conservative | Returned to the Conservative caucus. |
| March 13, 2008 | Maka Kotto | Saint-Lambert | Bloc Québécois | Vacated seat to run in a provincial by-election |
| March 17, 2008 | Rob Clarke | Desnethé—Missinippi—Churchill River | Conservative | Elected in a by-election |
| March 17, 2008 | Joyce Murray | Vancouver Quadra | Liberal | Elected in a by-election |
| March 17, 2008 | Martha Hall Findlay | Willowdale | Liberal | Elected in a by-election |
| March 17, 2008 | Bob Rae | Toronto Centre | Liberal | Elected in a by-election |
| April 7, 2008 | Brenda Chamberlain | Guelph | Liberal | Vacated seat |
| August 1, 2008 | John Godfrey | Don Valley West | Liberal | Vacated seat |
| August 30, 2008 | Blair Wilson | West Vancouver—Sunshine Coast—Sea to Sky Country | Green | Founded Green caucus. |