Auxilin

DNAJC6
Identifiers
Aliases	DNAJC6 , DJC6, PARK19, Auxilin, DnaJ heat shock protein family (Hsp40) member C6
External IDs	OMIM: 608375; MGI: 1919935; HomoloGene: 8865; GeneCards: DNAJC6; OMA:DNAJC6 - orthologs
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1p31.3 Start 65,248,219 bp [1] End 65,415,871 bp [1]
Gene location (Mouse) Chr. Chromosome 4 (mouse) [2] Band 4|4 C6 Start 101,353,828 bp [2] End 101,499,996 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in endothelial cell Brodmann area 23 pars compacta pars reticulata orbitofrontal cortex middle temporal gyrus lateral nuclear group of thalamus pons inferior olivary nucleus Brodmann area 46 Top expressed in medial dorsal nucleus dorsomedial hypothalamic nucleus ventral tegmental area pontine nuclei medial geniculate nucleus paraventricular nucleus of hypothalamus lateral geniculate nucleus piriform cortex primary motor cortex central gray substance of midbrain More reference expression data BioGPS n/a
Gene ontology Molecular function protein tyrosine phosphatase activity SH3 domain binding phosphoprotein phosphatase activity hydrolase activity clathrin binding Cellular component cytosol synapse vesicle postsynaptic density cytoplasm intracellular membrane-bounded organelle presynapse Biological process peptidyl-tyrosine dephosphorylation regulation of clathrin-dependent endocytosis protein dephosphorylation clathrin coat disassembly receptor-mediated endocytosis membrane organization synaptic vesicle uncoating clathrin-dependent endocytosis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9829 72685 Ensembl ENSG00000116675 ENSMUSG00000028528 UniProt O75061 Q80TZ3 RefSeq (mRNA) NM_001256864 NM_001256865 NM_014787 NM_001164583 NM_001164584 NM_001164585 NM_198412 NM_001355179 NM_001355180 RefSeq (protein) NP_001243793 NP_001243794 NP_055602 NP_001158055 NP_001158056 NP_001158057 NP_940804 NP_001342108 NP_001342109 Location (UCSC) Chr 1: 65.25 – 65.42 Mb Chr 4: 101.35 – 101.5 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Putative tyrosine-protein phosphatase auxilin is an enzyme that in humans is encoded by the DNAJC6 gene. ^{[5] [6] [7]}

Function

DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc-finger domain (Ohtsuka and Hata, 2000). ^[7]

Structure

The protein tyrosine phosphatase domain and C2 domain pair of auxilin, located near the N-terminus of the polypeptide, constitute a superdomain, a tandem arrangement of two or more nominally unrelated domains that form a single heritable unit. ^[8] The phosphatase domain belongs to the auxilin subfamily of lipid phosphatases and is predicted to be catalytically inactive. ^{[9] [10]}

References

1. GRCh38: Ensembl release 89: ENSG00000116675 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000028528 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Seki N, Ohira M, Nagase T, Ishikawa K, Miyajima N, Nakajima D, Nomura N, Ohara O (October 1997). "Characterization of cDNA clones in size-fractionated cDNA libraries from human brain". DNA Research. 4 (5): 345–9. doi: 10.1093/dnares/4.5.345 . PMID   9455484.
6. Ohtsuka K, Hata M (April 2000). "Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature". Cell Stress & Chaperones. 5 (2): 98–112. doi:10.1379/1466-1268(2000)005<0098:mhdhco>2.0.co;2 (inactive 11 July 2025). PMC   312896 . PMID   11147971.
7. "Entrez Gene: DNAJC6 DnaJ (Hsp40) homolog, subfamily C, member 6".
8. Haynie DT, Xue B (May 2015). "Superdomains in the protein structure hierarchy: The case of PTP-C2". Protein Science. 24 (5): 874–82. doi:10.1002/pro.2664. PMC   4420535 . PMID   25694109.
9. "Phosphatase Subfamily Auxilin - PhosphataseWiki". phosphatome.net.
10. Chen MJ, Dixon JE, Manning G (April 2017). "Genomics and evolution of protein phosphatases". Science Signaling. 10 (474): eaag1796. doi:10.1126/scisignal.aag1796. PMID   28400531. S2CID   41041971.

External links

• Human DNAJC6 genome location and DNAJC6 gene details page in the UCSC Genome Browser .

Further reading

• Suzuki Y, Yamashita R, Shirota M, Sakakibara Y, Chiba J, Mizushima-Sugano J, Nakai K, Sugano S (September 2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions". Genome Research. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC   515316 . PMID   15342556.
• Scheele U, Alves J, Frank R, Duwel M, Kalthoff C, Ungewickell E (July 2003). "Molecular and functional characterization of clathrin- and AP-2-binding determinants within a disordered domain of auxilin". The Journal of Biological Chemistry. 278 (28): 25357–68. doi: 10.1074/jbc.M303738200 . PMID   12732633.
• Scheele U, Kalthoff C, Ungewickell E (September 2001). "Multiple interactions of auxilin 1 with clathrin and the AP-2 adaptor complex". The Journal of Biological Chemistry. 276 (39): 36131–8. doi: 10.1074/jbc.M106511200 . PMID   11470803.
• Ishikawa K, Nagase T, Nakajima D, Seki N, Ohira M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (October 1997). "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 4 (5): 307–13. doi: 10.1093/dnares/4.5.307 . PMID   9455477.