CDC34

CDC34
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2OB4, 3RZ3, 4MDK
Identifiers
Aliases	CDC34 , E2-UBC3, UBCH3, UBE2R1, cell division cycle 34, cell division cycle 34, ubiqiutin conjugating enzyme
External IDs	OMIM: 116948; MGI: 102657; HomoloGene: 55815; GeneCards: CDC34; OMA:CDC34 - orthologs
EC number	2.3.2.24
Gene location (Human) Chr. Chromosome 19 (human) [1] Band 19p13.3 Start 531,760 bp [1] End 542,092 bp [1]
Gene location (Mouse) Chr. Chromosome 10 (mouse) [2] Band 10 C1|10 39.72 cM Start 79,518,029 bp [2] End 79,524,232 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in left testis right testis apex of heart ventricular zone right hemisphere of cerebellum muscle layer of sigmoid colon muscle of thigh ganglionic eminence right lobe of liver gastrocnemius muscle Top expressed in quadriceps femoris muscle muscle of thigh muscle tissue skeletal muscle tissue epiblast testicle placenta lip white adipose tissue yolk sac More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity nucleotide binding ubiquitin protein ligase activity ubiquitin-protein transferase activity protein binding ATP binding ubiquitin conjugating enzyme activity ubiquitin protein ligase binding Cellular component nucleus nucleoplasm cytoplasm cytosol nuclear speck Biological process negative regulation of cAMP-mediated signaling cellular response to interferon-beta protein polyubiquitination positive regulation of inclusion body assembly protein K48-linked ubiquitination positive regulation of neuron apoptotic process DNA replication initiation cell cycle response to growth factor proteasome-mediated ubiquitin-dependent protein catabolic process protein ubiquitination G1/S transition of mitotic cell cycle ubiquitin-dependent protein catabolic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 997 216150 Ensembl ENSG00000099804 ENSMUSG00000020307 UniProt P49427 Q8CFI2 RefSeq (mRNA) NM_004359 NM_177613 NM_001359817 NM_001359818 RefSeq (protein) NP_004350 NP_808281 NP_001346746 NP_001346747 Location (UCSC) Chr 19: 0.53 – 0.54 Mb Chr 10: 79.52 – 79.52 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

CDC34 is a gene that in humans encodes the protein Ubiquitin-conjugating enzyme E2 R1. ^{[5] [6] [7]} This protein is a member of the ubiquitin-conjugating enzyme family, which catalyzes the covalent attachment of ubiquitin to other proteins. ^[8]

CDC34 was originally discovered by work in baker's yeast as a gene that is essential for the cell cycle. ^[9] Cdc34 in yeast targets numerous substrates - notably the cyclin-dependent kinase inhibitor Sic1 ^[10] - for ubiquitin-mediated protein degradation. ^[11] CDC34 is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. ^[7]

Interactions

CDC34 has been shown to interact with CSNK2B, ^[12] BTRC ^{[13] [14]} and CDK9. ^[15]

References

1. GRCh38: Ensembl release 89: ENSG00000099804 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020307 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Plon SE, Leppig KA, Do HN, Groudine M (November 1993). "Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast". Proceedings of the National Academy of Sciences of the United States of America. 90 (22): 10484–8. Bibcode:1993PNAS...9010484P. doi: 10.1073/pnas.90.22.10484 . PMC   47801 . PMID   8248134.
6. Gazdoiu S, Yamoah K, Wu K, Escalante CR, Tappin I, Bermudez V, et al. (October 2005). "Proximity-induced activation of human Cdc34 through heterologous dimerization". Proceedings of the National Academy of Sciences of the United States of America. 102 (42): 15053–8. Bibcode:2005PNAS..10215053G. doi: 10.1073/pnas.0507646102 . PMC   1242854 . PMID   16210246.
7. "Entrez Gene: CDC34 cell division cycle 34 homolog (S. cerevisiae)".
8. Goebl MG, Yochem J, Jentsch S, McGrath JP, Varshavsky A, Byers B (September 1988). "The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme". Science. 241 (4871): 1331–5. Bibcode:1988Sci...241.1331G. doi:10.1126/science.2842867. PMID   2842867.
9. Dutcher SK, Hartwell LH (February 1982). "The role of S. cerevisiae cell division cycle genes in nuclear fusion". Genetics. 100 (2): 175–84. doi:10.1093/genetics/100.2.175. PMC   1201806 . PMID   7049831.
10. King RW, Deshaies RJ, Peters JM, Kirschner MW (December 1996). "How proteolysis drives the cell cycle". Science. 274 (5293): 1652–9. Bibcode:1996Sci...274.1652K. doi:10.1126/science.274.5293.1652. PMID   8939846. S2CID   25369228.
11. Williams KM, Qie S, Atkison JH, Salazar-Arango S, Alan Diehl J, Olsen SK (July 2019). "Structural insights into E1 recognition and the ubiquitin-conjugating activity of the E2 enzyme Cdc34". Nature Communications. 10 (1): 3296. Bibcode:2019NatCo..10.3296W. doi: 10.1038/s41467-019-11061-8 . PMC   6656757 . PMID   31341161.
12. Block K, Boyer TG, Yew PR (November 2001). "Phosphorylation of the human ubiquitin-conjugating enzyme, CDC34, by casein kinase 2". The Journal of Biological Chemistry. 276 (44): 41049–58. doi: 10.1074/jbc.M106453200 . PMID   11546811.
13. Semplici F, Meggio F, Pinna LA, Oliviero S (June 2002). "CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation". Oncogene. 21 (25): 3978–87. doi: 10.1038/sj.onc.1205574 . PMID   12037680.
14. Cenciarelli C, Chiaur DS, Guardavaccaro D, Parks W, Vidal M, Pagano M (October 1999). "Identification of a family of human F-box proteins". Current Biology. 9 (20): 1177–9. Bibcode:1999CBio....9.1177C. doi: 10.1016/S0960-9822(00)80020-2 . PMID   10531035. S2CID   7467493.
15. Kiernan RE, Emiliani S, Nakayama K, Castro A, Labbé JC, Lorca T, et al. (December 2001). "Interaction between cyclin T1 and SCF(SKP2) targets CDK9 for ubiquitination and degradation by the proteasome". Molecular and Cellular Biology. 21 (23): 7956–70. doi:10.1128/MCB.21.23.7956-7970.2001. PMC   99964 . PMID   11689688.

External links

• Human CDC34 genome location and CDC34 gene details page in the UCSC Genome Browser .

Further reading

• King RW, Deshaies RJ, Peters JM, Kirschner MW (December 1996). "How proteolysis drives the cell cycle". Science. 274 (5293): 1652–9. Bibcode:1996Sci...274.1652K. doi:10.1126/science.274.5293.1652. PMID   8939846. S2CID   25369228.
• Pagano M (November 1997). "Cell cycle regulation by the ubiquitin pathway". FASEB Journal. 11 (13): 1067–75. doi: 10.1096/fasebj.11.13.9367342 . PMID   9367342. S2CID   6368478.
• Lisztwan J, Marti A, Sutterlüty H, Gstaiger M, Wirbelauer C, Krek W (January 1998). "Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34 with the F-box protein p45(SKP2): evidence for evolutionary conservation in the subunit composition of the CDC34-SCF pathway". The EMBO Journal. 17 (2): 368–83. doi:10.1093/emboj/17.2.368. PMC   1170388 . PMID   9430629.
• Song A, Wang Q, Goebl MG, Harrington MA (September 1998). "Phosphorylation of nuclear MyoD is required for its rapid degradation". Molecular and Cellular Biology. 18 (9): 4994–9. doi:10.1128/mcb.18.9.4994. PMC   109084 . PMID   9710583.
• Gonen H, Bercovich B, Orian A, Carrano A, Takizawa C, Yamanaka K, et al. (May 1999). "Identification of the ubiquitin carrier proteins, E2s, involved in signal-induced conjugation and subsequent degradation of IkappaBalpha". The Journal of Biological Chemistry. 274 (21): 14823–30. doi: 10.1074/jbc.274.21.14823 . PMID   10329681.
• Pati D, Meistrich ML, Plon SE (July 1999). "Human Cdc34 and Rad6B ubiquitin-conjugating enzymes target repressors of cyclic AMP-induced transcription for proteolysis". Molecular and Cellular Biology. 19 (7): 5001–13. doi:10.1128/mcb.19.7.5001. PMC   84326 . PMID   10373550.
• Seol JH, Feldman RM, Zachariae W, Shevchenko A, Correll CC, Lyapina S, et al. (June 1999). "Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34". Genes & Development. 13 (12): 1614–26. doi:10.1101/gad.13.12.1614. PMC   316801 . PMID   10385629.
• Read MA, Brownell JE, Gladysheva TB, Hottelet M, Parent LA, Coggins MB, et al. (April 2000). "Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha". Molecular and Cellular Biology. 20 (7): 2326–33. doi:10.1128/MCB.20.7.2326-2333.2000. PMC   85397 . PMID   10713156.
• Reymond F, Wirbelauer C, Krek W (May 2000). "Association of human ubiquitin-conjugating enzyme CDC34 with the mitotic spindle in anaphase". Journal of Cell Science. 113 ( Pt 10) (10): 1687–94. doi: 10.1242/jcs.113.10.1687 . PMID   10769200.
• Charrasse S, Carena I, Brondani V, Klempnauer KH, Ferrari S (June 2000). "Degradation of B-Myb by ubiquitin-mediated proteolysis: involvement of the Cdc34-SCF(p45Skp2) pathway". Oncogene. 19 (26): 2986–95. doi: 10.1038/sj.onc.1203618 . PMID   10871850.
• Strack P, Caligiuri M, Pelletier M, Boisclair M, Theodoras A, Beer-Romero P, et al. (July 2000). "SCF(beta-TRCP) and phosphorylation dependent ubiquitinationof I kappa B alpha catalyzed by Ubc3 and Ubc4". Oncogene. 19 (31): 3529–36. doi: 10.1038/sj.onc.1203647 . PMID   10918611.
• Duan W, Sun B, Li TW, Tan BJ, Lee MK, Teo TS (October 2000). "Cloning and characterization of AWP1, a novel protein that associates with serine/threonine kinase PRK1 in vivo". Gene. 256 (1–2): 113–21. doi:10.1016/S0378-1119(00)00365-6. PMID   11054541.
• Block K, Boyer TG, Yew PR (November 2001). "Phosphorylation of the human ubiquitin-conjugating enzyme, CDC34, by casein kinase 2". The Journal of Biological Chemistry. 276 (44): 41049–58. doi: 10.1074/jbc.M106453200 . PMID   11546811.
• Wu K, Chen A, Tan P, Pan ZQ (January 2002). "The Nedd8-conjugated ROC1-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34". The Journal of Biological Chemistry. 277 (1): 516–27. doi: 10.1074/jbc.M108008200 . PMID   11675391.
• Kiernan RE, Emiliani S, Nakayama K, Castro A, Labbé JC, Lorca T, et al. (December 2001). "Interaction between cyclin T1 and SCF(SKP2) targets CDK9 for ubiquitination and degradation by the proteasome". Molecular and Cellular Biology. 21 (23): 7956–70. doi:10.1128/MCB.21.23.7956-7970.2001. PMC   99964 . PMID   11689688.
• Semplici F, Meggio F, Pinna LA, Oliviero S (June 2002). "CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation". Oncogene. 21 (25): 3978–87. doi: 10.1038/sj.onc.1205574 . PMID   12037680.