HSPA9

HSPA9
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3N8E, 4KBO
Identifiers
Aliases	HSPA9 , CSA, GRP-75, GRP75, HEL-S-124m, HSPA9B, MOT, MOT2, MTHSP75, PBP74, CRP40, EVPLS, SAAN, SIDBA4, heat shock protein family A (Hsp70) member 9
External IDs	OMIM: 600548; MGI: 96245; HomoloGene: 39452; GeneCards: HSPA9; OMA:HSPA9 - orthologs
Gene location (Human) Chr. Chromosome 5 (human) [1] Band 5q31.2 Start 138,553,756 bp [1] End 138,575,675 bp [1]
Gene location (Mouse) Chr. Chromosome 18 (mouse) [2] Band 18 B1|18 18.8 cM Start 35,070,467 bp [2] End 35,087,410 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right adrenal gland right adrenal cortex left adrenal gland left adrenal cortex epithelium of colon muscle of thigh ventricular zone Achilles tendon gastrocnemius muscle islet of Langerhans Top expressed in Ileal epithelium primitive streak Paneth cell endothelial cell of lymphatic vessel right kidney epiblast medullary collecting duct interventricular septum muscle of thigh adrenal gland More reference expression data BioGPS n/a
Gene ontology Molecular function nucleotide binding unfolded protein binding protein binding ATP binding ubiquitin protein ligase binding RNA binding ATPase activity heat shock protein binding protein folding chaperone activity misfolded protein binding enzyme binding Cellular component cytoplasm focal adhesion nucleolus mitochondrion mitochondrial nucleoid extracellular exosome nucleus extracellular matrix mitochondrial matrix Biological process negative regulation of apoptotic process protein folding negative regulation of erythrocyte differentiation erythrocyte differentiation interleukin-12-mediated signaling pathway response to unfolded protein iron-sulfur cluster assembly cellular response to heat Unfolded Protein Response protein refolding regulation of erythrocyte differentiation chaperone cofactor-dependent protein refolding protein export from nucleus negative regulation of hematopoietic stem cell differentiation negative regulation of hemopoiesis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 3313 15526 Ensembl ENSG00000113013 ENSMUSG00000024359 UniProt P38646 P38647 RefSeq (mRNA) NM_004134 NM_010481 RefSeq (protein) NP_004125 NP_034611 Location (UCSC) Chr 5: 138.55 – 138.58 Mb Chr 18: 35.07 – 35.09 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Mitochondrial 70kDa heat shock protein (mtHsp70), also known as mortalin, is a protein that in humans is encoded by the HSPA9 gene. ^{[5] [6]}

Function

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein plays a role in the control of cell proliferation. It may also act as a chaperone. ^[6]

Interactions

HSPA9 has been shown to interact with FGF1 ^[7] and P53. ^[8]

Clinical relevance and genetic deficiency

In 2015, a group around Andrea Superti-Furga showed that biallelic variants in the HSPA9 gene may result in a combination of congenital malformations called the EVEN-PLUS syndrome. ^{[9] [10]} These genetic variants have been shown to interfere with normal HSPA9 function ^[11]

References

1. GRCh38: Ensembl release 89: ENSG00000113013 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000024359 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Domanico SZ, DeNagel DC, Dahlseid JN, Green JM, Pierce SK (Jun 1993). "Cloning of the gene encoding peptide-binding protein 74 shows that it is a new member of the heat shock protein 70 family". Mol Cell Biol. 13 (6): 3598–610. doi:10.1128/mcb.13.6.3598. PMC   359829 . PMID   7684501.
6. "Entrez Gene: HSPA9 heat shock 70kDa protein 9 (mortalin)".
7. Mizukoshi E, Suzuki M, Loupatov A, Uruno T, Hayashi H, Misono T, Kaul SC, Wadhwa R, Imamura T (Oct 1999). "Fibroblast growth factor-1 interacts with the glucose-regulated protein GRP75/mortalin". Biochem. J. 343 (2): 461–6. doi:10.1042/0264-6021:3430461. PMC   1220575 . PMID   10510314.
8. Wadhwa R, Yaguchi T, Hasan MK, Mitsui Y, Reddel RR, Kaul SC (Apr 2002). "Hsp70 family member, mot-2/mthsp70/GRP75, binds to the cytoplasmic sequestration domain of the p53 protein". Exp. Cell Res. 274 (2): 246–53. doi:10.1006/excr.2002.5468. PMID   11900485.
9. Royer-Bertrand B, Castillo-Taucher S, Moreno-Salinas R, Cho TJ, Chae JH, Choi M, et al. (November 2015). "Mutations in the heat-shock protein A9 (HSPA9) gene cause the EVEN-PLUS syndrome of congenital malformations and skeletal dysplasia". Scientific Reports. 5 17154. Bibcode:2015NatSR...517154R. doi:10.1038/srep17154. PMC   4657157 . PMID   26598328.
10. "MIM 616854: Even Plus Syndrome". OMIM.
11. Moseng MA, Nix JC, Page RC (April 2019). "Biophysical Consequences of EVEN-PLUS Syndrome Mutations for the Function of Mortalin". The Journal of Physical Chemistry B. 123 (16): 3383–3396. doi:10.1021/acs.jpcb.9b00071. PMC   6483861 . PMID   30933555.

Further reading

• Kaul SC, Taira K, Pereira-Smith OM, Wadhwa R (2003). "Mortalin: present and prospective". Exp. Gerontol. 37 (10–11): 1157–64. doi:10.1016/S0531-5565(02)00135-3. PMID   12470827. S2CID   44450296.
• Yaguchi T, Aida S, Kaul SC, Wadhwa R (2007). "Involvement of mortalin in cellular senescence from the perspective of its mitochondrial import, chaperone, and oxidative stress management functions". Ann. N. Y. Acad. Sci. 1100 (1): 306–11. Bibcode:2007NYASA1100..306Y. doi:10.1196/annals.1395.032. PMID   17460192. S2CID   43848246.
• Hochstrasser DF, Frutiger S, Paquet N, Bairoch A, Ravier F, Pasquali C, Sanchez JC, Tissot JD, Bjellqvist B, Vargas R (1993). "Human liver protein map: a reference database established by microsequencing and gel comparison". Electrophoresis. 13 (12): 992–1001. doi:10.1002/elps.11501301201. PMID   1286669. S2CID   23518983.
• Kaul SC, Wadhwa R, Matsuda Y, Hensler PJ, Pereira-Smith OM, Komatsu Y, Mitsui Y (1995). "Mouse and human chromosomal assignments of mortalin, a novel member of the murine hsp70 family of proteins". FEBS Lett. 361 (2–3): 269–72. Bibcode:1995FEBSL.361..269K. doi: 10.1016/0014-5793(95)00177-B . PMID   7698336. S2CID   36957259.
• Bhattacharyya T, Karnezis AN, Murphy SP, Hoang T, Freeman BC, Phillips B, Morimoto RI (1995). "Cloning and subcellular localization of human mitochondrial hsp70". J. Biol. Chem. 270 (4): 1705–10. doi: 10.1074/jbc.270.4.1705 . PMID   7829505.
• Furlini G, Vignoli M, Re MC, Gibellini D, Ramazzotti E, Zauli G, La Placa M (1994). "Human immunodeficiency virus type 1 interaction with the membrane of CD4+ cells induces the synthesis and nuclear translocation of 70K heat shock protein". J. Gen. Virol. 75 (1): 193–9. doi: 10.1099/0022-1317-75-1-193 . PMID   7906708.
• Matoba R, Okubo K, Hori N, Fukushima A, Matsubara K (1994). "The addition of 5'-coding information to a 3'-directed cDNA library improves analysis of gene expression". Gene. 146 (2): 199–207. doi:10.1016/0378-1119(94)90293-3. PMID   8076819.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Hughes GJ, Frutiger S, Paquet N, Pasquali C, Sanchez JC, Tissot JD, Bairoch A, Appel RD, Hochstrasser DF (1994). "Human liver protein map: update 1993". Electrophoresis. 14 (11): 1216–22. doi:10.1002/elps.11501401181. PMID   8313870. S2CID   33424554.
• Ji H, Reid GE, Moritz RL, Eddes JS, Burgess AW, Simpson RJ (1997). "A two-dimensional gel database of human colon carcinoma proteins". Electrophoresis. 18 (3–4): 605–13. doi:10.1002/elps.1150180344. PMID   9150948. S2CID   25454450.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Mizukoshi E, Suzuki M, Loupatov A, Uruno T, Hayashi H, Misono T, Kaul SC, Wadhwa R, Imamura T (1999). "Fibroblast growth factor-1 interacts with the glucose-regulated protein GRP75/mortalin". Biochem. J. 343 (2): 461–6. doi:10.1042/0264-6021:3430461. PMC   1220575 . PMID   10510314.
• O'Keeffe B, Fong Y, Chen D, Zhou S, Zhou Q (2000). "Requirement for a kinase-specific chaperone pathway in the production of a Cdk9/cyclin T1 heterodimer responsible for P-TEFb-mediated tat stimulation of HIV-1 transcription". J. Biol. Chem. 275 (1): 279–87. doi: 10.1074/jbc.275.1.279 . PMID   10617616.
• Kaula SC, Reddelb RR, Sugiharac T, Mitsuia Y, Wadhwac R (2000). "Inactivation of p53 and life span extension of human diploid fibroblasts by mot-2". FEBS Lett. 474 (2–3): 159–64. Bibcode:2000FEBSL.474..159K. doi: 10.1016/S0014-5793(00)01594-5 . PMID   10838077. S2CID   85853656.
• Agostini I, Popov S, Li J, Dubrovsky L, Hao T, Bukrinsky M (2000). "Heat-shock protein 70 can replace viral protein R of HIV-1 during nuclear import of the viral preintegration complex". Exp. Cell Res. 259 (2): 398–403. doi:10.1006/excr.2000.4992. PMID   10964507.
• Xie H, Hu Z, Chyna B, Horrigan SK, Westbrook CA (2001). "Human mortalin (HSPA9): a candidate for the myeloid leukemia tumor suppressor gene on 5q31". Leukemia. 14 (12): 2128–34. doi: 10.1038/sj.leu.2401935 . PMID   11187902.
• Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. Bibcode:2002CBio...12....1A. doi: 10.1016/S0960-9822(01)00650-9 . PMID   11790298. S2CID   14132033.
• Carette J, Lehnert S, Chow TY (2002). "Implication of PBP74/mortalin/GRP75 in the radio-adaptive response". Int. J. Radiat. Biol. 78 (3): 183–90. doi:10.1080/09553000110097208. PMID   11869473. S2CID   536461.
• Wadhwa R, Yaguchi T, Hasan MK, Mitsui Y, Reddel RR, Kaul SC (2002). "Hsp70 family member, mot-2/mthsp70/GRP75, binds to the cytoplasmic sequestration domain of the p53 protein". Exp. Cell Res. 274 (2): 246–53. doi:10.1006/excr.2002.5468. PMID   11900485.

External links

• HSPA9+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
• PDBe-KB provides an overview of all the structure information available in the PDB for Human Stress-70 protein, mitochondrial