DDX5

DDX5
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3FE2, 4A4D
Identifiers
Aliases	DDX5 , G17P1, HLR1, HUMP68, p68, DEAD-box helicase 5
External IDs	OMIM: 180630; MGI: 105037; HomoloGene: 6797; GeneCards: DDX5; OMA:DDX5 - orthologs
Gene location (Human) Chr. Chromosome 17 (human) [1] Band 17q23.3 Start 64,498,254 bp [1] End 64,508,199 bp [1]
Gene location (Mouse) Chr. Chromosome 11 (mouse) [2] Band 11 E1|11 70.01 cM Start 106,671,181 bp [2] End 106,680,011 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Achilles tendon bone marrow cells granulocyte anterior pituitary right adrenal cortex smooth muscle tissue epithelium of colon gonad monocyte right lobe of thyroid gland Top expressed in lobe of cerebellum cerebellar vermis neural layer of retina olfactory tubercle granulocyte habenula median eminence pineal gland arcuate nucleus lateral septal nucleus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding helicase activity calcium-dependent protein binding calmodulin binding protein binding androgen receptor binding nucleic acid binding enzyme binding pre-mRNA binding hydrolase activity ATP binding RNA helicase activity RNA binding mRNA 3'-UTR binding ribonucleoprotein complex binding promoter-specific chromatin binding MH2 domain binding SMAD binding R-SMAD binding primary miRNA binding transcription coregulator activity Cellular component catalytic step 2 spliceosome membrane nucleolus spliceosomal complex extracellular exosome nucleus extracellular matrix nucleoplasm SMAD protein complex cytoplasm ribonucleoprotein complex Biological process regulation of skeletal muscle cell differentiation regulation of transcription, DNA-templated rhythmic process regulation of viral genome replication mRNA processing mRNA transcription negative regulation of transcription by RNA polymerase II transcription, DNA-templated cell growth regulation of osteoblast differentiation RNA secondary structure unwinding intrinsic apoptotic signaling pathway by p53 class mediator RNA splicing regulation of androgen receptor signaling pathway positive regulation of DNA damage response, signal transduction by p53 class mediator mRNA splicing, via spliceosome regulation of alternative mRNA splicing, via spliceosome nuclear-transcribed mRNA catabolic process alternative mRNA splicing, via spliceosome regulation of transcription by RNA polymerase II intracellular estrogen receptor signaling pathway androgen receptor signaling pathway epithelial to mesenchymal transition myoblast differentiation pri-miRNA transcription by RNA polymerase II BMP signaling pathway regulation of pri-miRNA transcription by RNA polymerase II positive regulation of production of miRNAs involved in gene silencing by miRNA Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1655 13207 Ensembl ENSG00000108654 ENSMUSG00000020719 UniProt P17844 Q61656 RefSeq (mRNA) NM_004396 NM_001320595 NM_001320596 NM_001320597 NM_007840 NM_001355676 NM_001355677 RefSeq (protein) NP_001307524 NP_001307525 NP_001307526 NP_004387 n/a Location (UCSC) Chr 17: 64.5 – 64.51 Mb Chr 11: 106.67 – 106.68 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Probable ATP-dependent RNA helicase DDX5 also known as DEAD box protein 5 or RNA helicase p68 is an enzyme that in humans is encoded by the DDX5 gene. ^[5]

Function

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, ^[6] and cellular growth and division. This gene encodes a DEAD box protein, which is an RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. ^[5]

Interactions

DDX5 has been shown to interact with:

• AKAP8, ^[7]
• DDX17 (p72), ^[8]
• DHX9 (RNA helicase A), ^[9]
• Estrogen receptor alpha, ^[10]
• Fibrillarin, ^[11]
• HDAC1, ^[12]
• p53, ^[13]
• CTCF. ^[14]

References

1. GRCh38: Ensembl release 89: ENSG00000108654 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020719 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: DDX5 DEAD (Asp-Glu-Ala-Asp) box polypeptide 5".
6. Legrand J, Chan AL, La HM, Rossello FJ, Änkö ML, Fuller-Pace FV, Hobbs RM (23 May 2019). "DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia". Nature Communications. 10 (1): 2278. Bibcode:2019NatCo..10.2278L. doi:10.1038/s41467-019-09972-7. PMC   6533336 . PMID   31123254.
7. Akileswaran L, Taraska JW, Sayer JA, Gettemy JM, Coghlan VM (May 2001). "A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase". J. Biol. Chem. 276 (20): 17448–54. doi: 10.1074/jbc.M101171200 . PMID   11279182.
8. Ogilvie VC, Wilson BJ, Nicol SM, Morrice NA, Saunders LR, Barber GN, Fuller-Pace FV (March 2003). "The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells". Nucleic Acids Res. 31 (5): 1470–80. doi:10.1093/nar/gkg236. PMC   149829 . PMID   12595555.
9. Wilson BJ, Giguère V (2007). "Identification of novel pathway partners of p68 and p72 RNA helicases through Oncomine meta-analysis". BMC Genomics. 8: 419. doi: 10.1186/1471-2164-8-419 . PMC   3225811 . PMID   18005418.
10. Rossow KL, Janknecht R (January 2003). "Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300". Oncogene. 22 (1): 151–6. doi: 10.1038/sj.onc.1206067 . PMID   12527917.
11. Nicol SM, Causevic M, Prescott AR, Fuller-Pace FV (June 2000). "The nuclear DEAD box RNA helicase p68 interacts with the nucleolar protein fibrillarin and colocalizes specifically in nascent nucleoli during telophase". Exp. Cell Res. 257 (2): 272–80. doi:10.1006/excr.2000.4886. PMID   10837141.
12. Wilson BJ, Bates GJ, Nicol SM, Gregory DJ, Perkins ND, Fuller-Pace FV (August 2004). "The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner". BMC Mol. Biol. 5: 11. doi: 10.1186/1471-2199-5-11 . PMC   514542 . PMID   15298701.
13. Bates GJ, Nicol SM, Wilson BJ, Jacobs AM, Bourdon JC, Wardrop J, Gregory DJ, Lane DP, Perkins ND, Fuller-Pace FV (February 2005). "The DEAD box protein p68: a novel transcriptional coactivator of the p53 tumour suppressor". EMBO J. 24 (3): 543–53. doi:10.1038/sj.emboj.7600550. PMC   548656 . PMID   15660129.
14. Yao H, Brick K, Evrard Y, Xiao T, Camerini-Otero RD, Felsenfeld G (2010). "Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA". Genes Dev. 24 (22): 2543–2555. doi:10.1101/gad.1967810. PMC   2975930 . PMID   20966046.

Further reading

• Iggo RD, Jamieson DJ, MacNeill SA, et al. (1991). "p68 RNA helicase: identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts". Mol. Cell. Biol. 11 (3): 1326–33. doi:10.1128/MCB.11.3.1326. PMC   369403 . PMID   1996094.
• Hloch P, Schiedner G, Stahl H (1990). "Complete cDNA sequence of the human p68 protein". Nucleic Acids Res. 18 (10): 3045. doi:10.1093/nar/18.10.3045. PMC   330836 . PMID   2349099.
• Ford MJ, Anton IA, Lane DP (1988). "Nuclear protein with sequence homology to translation initiation factor eIF-4A". Nature. 332 (6166): 736–8. Bibcode:1988Natur.332..736F. doi:10.1038/332736a0. PMID   2451786. S2CID   4351448.
• Iggo R, Gough A, Xu W, et al. (1989). "Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction". Proc. Natl. Acad. Sci. U.S.A. 86 (16): 6211–4. Bibcode:1989PNAS...86.6211I. doi: 10.1073/pnas.86.16.6211 . PMC   297807 . PMID   2762324.
• Buelt MK, Glidden BJ, Storm DR (1994). "Regulation of p68 RNA helicase by calmodulin and protein kinase C". J. Biol. Chem. 269 (47): 29367–70. doi: 10.1016/S0021-9258(18)43885-9 . PMID   7525583.
• Brody LC, Abel KJ, Castilla LH, et al. (1995). "Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17". Genomics. 25 (1): 238–47. doi: 10.1016/0888-7543(95)80131-5 . PMID   7774924.
• Rössler OG, Hloch P, Schütz N, et al. (2000). "Structure and expression of the human p68 RNA helicase gene". Nucleic Acids Res. 28 (4): 932–9. doi:10.1093/nar/28.4.932. PMC   102575 . PMID   10648785.
• Nicol SM, Causevic M, Prescott AR, Fuller-Pace FV (2000). "The nuclear DEAD box RNA helicase p68 interacts with the nucleolar protein fibrillarin and colocalizes specifically in nascent nucleoli during telophase". Exp. Cell Res. 257 (2): 272–80. doi:10.1006/excr.2000.4886. PMID   10837141.
• Akileswaran L, Taraska JW, Sayer JA, et al. (2001). "A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase". J. Biol. Chem. 276 (20): 17448–54. doi: 10.1074/jbc.M101171200 . PMID   11279182.
• Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. Bibcode:2002CBio...12....1A. doi: 10.1016/S0960-9822(01)00650-9 . PMID   11790298. S2CID   14132033.
• Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC   1370266 . PMID   11991638.
• Liu ZR (2002). "p68 RNA Helicase Is an Essential Human Splicing Factor That Acts at the U1 snRNA-5′ Splice Site Duplex". Mol. Cell. Biol. 22 (15): 5443–50. doi:10.1128/MCB.22.15.5443-5450.2002. PMC   133941 . PMID   12101238.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Rossow KL, Janknecht R (2003). "Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300". Oncogene. 22 (1): 151–6. doi: 10.1038/sj.onc.1206067 . PMID   12527917.
• Guil S, Gattoni R, Carrascal M, et al. (2003). "Roles of hnRNP A1, SR Proteins, and p68 Helicase in c-H-ras Alternative Splicing Regulation". Mol. Cell. Biol. 23 (8): 2927–41. doi:10.1128/MCB.23.8.2927-2941.2003. PMC   152554 . PMID   12665590.
• Fujita T, Kobayashi Y, Wada O, et al. (2003). "Full activation of estrogen receptor alpha activation function-1 induces proliferation of breast cancer cells". J. Biol. Chem. 278 (29): 26704–14. doi: 10.1074/jbc.M301031200 . PMID   12738788.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.