Cyclic neutropenia

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Cyclic neutropenia
Other namesPeriodic neutropenia, cyclic leucopenia, cyclic hematopoesis
Neutropenia.JPG
Blood film with a striking absence of neutrophils, leaving only red blood cells and platelets.
Specialty Hematology   OOjs UI icon edit-ltr-progressive.svg
Symptoms Fever, malaise, inflammation and infection of oral mucosa, respiratory tract, digestive tract, skin, and abdominal pain [1]
Usual onsetInfancy [1]
CausesMutation in ELANE gene [1]
Diagnostic method Blood test, genetic testing [1]
Treatment G-CSF [1]
Medication Filgrastim [1]
Frequency1 in million (2018) [1]

Cyclic neutropenia (CyN) is a rare hematologic disorder and form of congenital neutropenia that tends to occur approximately every three weeks and lasting for few days at a time due to changing rates of neutrophil production by the bone marrow. It causes a temporary condition with a low absolute neutrophil count and because the neutrophils make up the majority of circulating white blood cells it places the body at severe risk of inflammation and infection. In comparison to severe congenital neutropenia, it responds well to treatment with granulocyte colony-stimulating factor (filgrastim), which increases the neutrophil count, shortens the cycle length, as well decreases the severity and frequency of infections.

Contents

Signs and symptoms

The common symptoms of neutropenia are recurrent fever, malaise, inflammation of the tissues surrounding the teeth, mouth ulcers, inflammation and bacterial infection of the respiratory tract, digestive tract, skin, and abdominal pain. [1] [2] It is considered that the greatest risk for death is from developing necrotizing enterocolitis (NEC), peritonitis, bacteremia or Clostridium and Escherichia coli sepsis and septic shock, and pneumonia. [1] [3] [4]

Causes

Cyclic neutropenia (CyN), like severe congenital neutropenia (SCN), is a rare disorder. It is considered that in the general population, CyN has a frequency of one in one million. [1] It is the result of autosomal dominant mutation in ELANE gene located on the short arm (p) of chromosome 19 (19p13.3), the gene encoding neutrophil elastase, which is also the most common cause of the SCN. [1] [5] [6] [7] It sporadically occurs as a de novo mutation variant or can be present among members of the same family. [1] In the case of CyN, the mutation variants have been found to mostly cluster in intron 4 and exon 4 and 5, [6] [3] but were also located in intron 3, and exon 2 and 3. [8] [9] [10] [11] [12] Some mutation variants have been found in both Cyn and SCN, which indicates they are phenotypes of the same disease with different severity. [9]

It is considered that the mutation causes a decrease in the "neutrophil production or excessive apoptosis (shorter half-life)" which results in a deficiency of mature neutrophils in the blood. [13] The exact pathological mechanism is still researched, with the main hypotheses being mislocalization of ELANE or unfolded protein response (UPR) induced by mutant ELANE, [14] however according to Mehta et al. (2016), the "UPR induction by mutant ELANE is not strong enough to promote cell death and that mutant ELANE causes SCN through an alternative mechanism". [15] According to Garg et al. (2020), new "findings challenge the currently prevailing model that SCN results from mutant ELANE, which triggers endoplasmic reticulum stress, UPR, and apoptosis". [16] The expression of the ELANE gene has been linked to GFI1 gene, [8] [17] and some considered that interaction with other genes causes the emergence and severity of one or the other phenotypic disorder. [9] It is unclear what causes the cyclic aspect in CyN. [17] According to Donadieu et al. (2011), the "cyclic aspect ... suggests the existence of a cryptic biological clock that regulates granulopoiesis. This putative clock might be revealed by particular mutations". [13] Michael Mackey "postulates that the production of neutrophils is governed by long‐range stimulatory factors in a long feedback loop that has a built‐in time delay in the maturation of promyelocytes to fully differentiated neutrophils". [18] It is also not clear what causes that the levels of secretory leucocyte protease inhibitor (SLPI), which influences the induction of the unfolded protein response (UPR), are not diminished and as such activation of UPR is absent in CyN compared to SCN, in other words, different ELANE mutations "have different effects on UPR activation, and SLPI regulates the extent of ELANE‐triggered UPR". [19]

"A hypothesis of UPR-induced cycling of hematopoiesis. Schematic of the relationship between peripheral blood ANC (purple line) and UPR intensity in bone marrow HSCs and progenitor cells of CyN patients", per Mir et al. (2020). Cyclic activity in cyclic neutropenia.jpg
"A hypothesis of UPR‐induced cycling of hematopoiesis. Schematic of the relationship between peripheral blood ANC (purple line) and UPR intensity in bone marrow HSCs and progenitor cells of CyN patients", per Mir et al. (2020).

A 2020 study published in Annals of the New York Academy of Sciences about the pathomechanism of CyN revealed that "some HSPCs escape the UPR‐induced endoplasmic reticulum (ER) stress and proliferate in response to granulocyte colony‐stimulating factor (G‐CSF) to a certain threshold at which UPR again affects the majority of HSPCs. There is a cyclic balance between ER stress-induced apoptosis of HSPCs and compensatory G‐CSF–stimulated HSPC proliferation followed by granulocytic differentiation"; in other words, CyN is "characterized by cycling UPR activities and cycling UPR‐escaping cells". Also, the most probable reason that from the same mutation variant develops SCN and not CyN is due to more severe damage caused by UPR stress in SCN. [18]

Diagnosis

A diagnosis is usually confirmed by monitoring absolute neutrophil (ANC) count three times per week for at least six weeks. [1] [3] The confirmation can be assisted with Lomb periodogram. [20] During the condition, which lasts for three to six days and tends to occur approximately every three weeks (but can range from 14 to 36 days), [2] [3] the absolute neutrophil count (ANC) is less than 200-500 cells/μL (<0.2-0.5x109/L), with increase of monocyte counts, and mild oscillations of other cells, including a mild anemia. [1] [21] Between cycles the neutrophil count mostly peaks at subnormal or normal values. [17]

Genetic testing is advised for mutations in the ELANE and other neutropenia related genes (like HAX1, G6PC3, GFI1 etc.) to differentiate it from other secondary causes and forms of neutropenia. [1] [22] In some cases intervals and oscillations can be lower, making the ANC analysis insufficient, [1] and since both disorders can have the same mutation variants in ELANE it is preferable to have both ANC and genetic analysis to confirm in the diagnosis whether it is severe congenital or cyclic neutropenia. [21] [20]

Treatment

"Cycling peripheral blood ANCs in CyN patients. Time course of ANC numbers in one CyN patient after initiation of G-CSF therapy", per Mir et al. (2020). Cycling absolute neutrophil count in cyclic neutropenia.jpg
"Cycling peripheral blood ANCs in CyN patients. Time course of ANC numbers in one CyN patient after initiation of G‐CSF therapy", per Mir et al. (2020).

Although individuals between cycles are generally healthy and symptoms tend to improve in adulthood, it is advised avoiding activities prone to injuries, to have regular oral and dental care, [2] and BCG vaccine to be avoided. [1] [13] It is advised monitoring white blood cells several times a year. The treatment following the symptoms should be immediate to prevent infections, especially during a fever when it requires broad-spectrum antibiotic therapy (see febrile neutropenia). The most important and often life-saving treatment is the preventive therapy of granulocyte colony-stimulating factor (G-CSF), in the form of filgrastim, which regulates the production of neutrophils within the bone marrow, but shortens the neutropenic cycle to about 7-14 days and the duration of the severe condition. [1] [17] The subcutaneous injections, with median dosage of 1.5 μg/kg/day, [18] can be given daily, intermittently once every three days, or timed to just treat the neutropenic period. [13] [23] The therapy is considered to be "safe and effective", with no significant adverse effects, [24] besides a possibility of development of osteopenia. [21]

The granulocyte-macrophage colony-stimulating factor (GM-CSF) is less effective with more adverse effects. Another alternative is hematopoietic stem cell transplantation (HSCT), but is usually practiced in SCN, [1] and in one case between two sibling donors, one of which was undergoing HSCT treatment for acute myeloid leukemia (AML) while the second had CyN and whose marrow was transferred, was also transferred CyN through allogeneic marrow grafting. It shows that CyN is a stem cell disorder. [25] Yearly bone marrow examinations are not recommended. [23]

Prognosis

There is a very high risk of life-threatening infections and death at an early age. [13] The quality of life and survival greatly improves with G-CSF treatment, which is practiced since the late 1980s. [21] Unlike severe congenital neutropenia, individuals with cyclic neutropenia have a better response to G-CSF and do not have a risk of developing myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). [1] [3] However, in long-term observation of over 300 patients with CyN, there has been one case of developing chronic myelogenous leukemia (CML) and one of AML, [21] [24] indicating it is also a pre-leukemic condition, but the risk is "very low" (1% [18] ), [26] and the risk is "correlated with disease severity rather than with occurrence of an ELANE mutation". [10] According to Donadieu et al. (2011), "the cumulative risk of experiencing at least one serious (potentially life-threatening) infection by age 20 years is similar in patients with permanent and cyclic neutropenia, although the former patients tend to have earlier manifestations". [13]

History

First described in 1910, [21] [2] it was suggested and confirmed to have an autosomal dominant (AD) inheritance in the 1940s and 1960s, [27] but was differentiated from congenital neutropenias until the 1990s when were analyzed pedigrees and identified genetic mutations shared by patients with severe congenital neutropenia. [13]

See also

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References

  1. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Dale DC, Makaryan V (2018) [2002]. Adam MP, Ardinger HH, Pagon RA, Wallace SE, Bean LJ (eds.). "ELANE-Related Neutropenia". GeneReviews . PMID   20301705.
  2. 1 2 3 4 Olvera KI, Barrios VM, Ríos RF, Ruidíaz VC (2015). "Cyclic neutropenia. Clinical case report". Revista Odontológica Mexicana. 19 (4): 246–252. doi: 10.1016/j.rodmex.2015.10.015 .
  3. 1 2 3 4 5 Boxer LA (2012). "How to approach neutropenia". Hematology. American Society of Hematology. Education Program. 2012 (1): 174–82. doi: 10.1182/asheducation.v2012.1.174.3798251 . PMID   23233578.
  4. ↑ James WD, Elston DM, Berger TG, Andrews GC (2006). Andrews' Diseases of the Skin: Clinical Dermatology . Saunders Elsevier. p.  808–811. ISBN   978-0-7216-2921-6.
  5. ↑ Sera Y, Kawaguchi H, Nakamura K, Sato T, Habara M, Okada S, et al. (August 2005). "A comparison of the defective granulopoiesis in childhood cyclic neutropenia and in severe congenital neutropenia". Haematologica. 90 (8): 1032–41. PMID   16079102.
  6. 1 2 Makaryan V, Zeidler C, Bolyard AA, Skokowa J, Rodger E, Kelley ML, et al. (January 2015). "The diversity of mutations and clinical outcomes for ELANE-associated neutropenia". Current Opinion in Hematology. 22 (1): 3–11. doi:10.1182/blood.V118.21.3398.3398. PMC   4380169 . PMID   25427142.
  7. ↑ Horwitz MS, Corey SJ, Grimes HL, Tidwell T (February 2013). "ELANE mutations in cyclic and severe congenital neutropenia: genetics and pathophysiology". Hematology/Oncology Clinics of North America. 27 (1): 19–41, vii. doi:10.1016/j.hoc.2012.10.004. PMC   3559001 . PMID   23351986.
  8. 1 2 Bellanné-Chantelot C, Clauin S, Leblanc T, Cassinat B, Rodrigues-Lima F, Beaufils S, et al. (June 2004). "Mutations in the ELA2 gene correlate with more severe expression of neutropenia: a study of 81 patients from the French Neutropenia Register". Blood. 103 (11): 4119–25. doi: 10.1182/blood-2003-10-3518 . PMID   14962902.
  9. 1 2 3 Newburger PE, Pindyck TN, Zhu Z, Bolyard AA, Aprikyan AA, Dale DC, et al. (August 2010). "Cyclic neutropenia and severe congenital neutropenia in patients with a shared ELANE mutation and paternal haplotype: evidence for phenotype determination by modifying genes". Pediatric Blood & Cancer. 55 (2): 314–7. doi:10.1002/pbc.22537. PMC   2913300 . PMID   20582973.
  10. 1 2 Germeshausen M, Deerberg S, Peter Y, Reimer C, Kratz CP, Ballmaier M (June 2013). "The spectrum of ELANE mutations and their implications in severe congenital and cyclic neutropenia". Human Mutation. 34 (6): 905–14. doi: 10.1002/humu.22308 . PMID   23463630. S2CID   3484630.
  11. ↑ Makaryan V, Zeidler C, Bolyard AA, Skokowa J, Rodger E, Kelley ML, et al. (January 2015). "The diversity of mutations and clinical outcomes for ELANE-associated neutropenia". Current Opinion in Hematology. 22 (1): 3–11. doi:10.1097/MOH.0000000000000105. PMC   4380169 . PMID   25427142.
  12. ↑ Liu Y, Fu J, Zhang J, Wang Y, Guan X (2017). "A Case Report on Recurrent Oral Ulcers Associated with Cyclic Neutropenia". Annals of Clinical Case Reports. 2: 905–914. ISSN   2474-1655. S2CID   21701463.
  13. 1 2 3 4 5 6 7 Donadieu J, Fenneteau O, Beaupain B, Mahlaoui N, Chantelot CB (May 2011). "Congenital neutropenia: diagnosis, molecular bases and patient management". Orphanet Journal of Rare Diseases. 6: 26. doi: 10.1186/1750-1172-6-26 . PMC   3127744 . PMID   21595885.
  14. ↑ Horwitz MS, Laurino MY, Keel SB (August 2019). "ELANE whole gene deletion mutation". Blood Advances. 3 (16): 2470–2473. doi:10.1182/bloodadvances.2019000498. PMC   6712528 . PMID   31427279.
  15. ↑ Mehta HM, Das A, Kamel R, Horwitz M, Corey S (December 2016). "Conditional Expression of Mutant ELANE Produces Unfolded Protein Response but Fails to Promote Cell Death or Differentiation Block: What Is the Mechanism for Severe Congenital Neutropenia?". Blood. 128 (22): 3899. doi: 10.1182/blood.V128.22.3899.3899 .
  16. ↑ Garg B, Mehta HM, Wang B, Kamel R, Horwitz MS, Corey SJ (May 2020). "ELANE mutation impairs granulocytic differentiation, without eliciting an unfolded protein response". The Journal of Biological Chemistry. 295 (21): 7492–7500. doi: 10.1074/jbc.RA120.012366 . PMC   7247317 . PMID   32299910.
  17. 1 2 3 4 Horwitz MS, Duan Z, Korkmaz B, Lee HH, Mealiffe ME, Salipante SJ (March 2007). "Neutrophil elastase in cyclic and severe congenital neutropenia". Blood. 109 (5): 1817–24. doi:10.1182/blood-2006-08-019166. PMC   1801070 . PMID   17053055.
  18. 1 2 3 4 5 6 Mir P, Klimiankou M, Findik B, Hähnel K, Mellor-Heineke S, Zeidler C, et al. (April 2020). "New insights into the pathomechanism of cyclic neutropenia". Annals of the New York Academy of Sciences. 1466 (1): 83–92. Bibcode:2020NYASA1466...83M. doi: 10.1111/nyas.14309 . PMID   32083314.
  19. ↑ Nustede R, Klimiankou M, Klimenkova O, Kuznetsova I, Zeidler C, Welte K, Skokowa J (January 2016). "ELANE mutant-specific activation of different UPR pathways in congenital neutropenia". British Journal of Haematology. 172 (2): 219–27. doi: 10.1111/bjh.13823 . PMID   26567890.
  20. 1 2 Dale DC, Bolyard AA, Leung J, Tran E, Marrero TM, Newburger PE (2017). "Cyclic Neutropenia, Congenital and Idiopathic Neutropenia". Blood . 130: 2275. Retrieved 2019-08-12.
  21. 1 2 3 4 5 6 Dale DC, Bolyard AA, Marrero TM, Bonilla MA, Link DC, Newburger PE, Shimamura A, Boxer LA (2012). "The Natural History of Cyclic Neutropenia: Long-Term Prospective Observations and Current Perspectives". Blood . 120 (21): 2141. doi:10.1182/blood.V120.21.2141.2141 . Retrieved 2019-08-12.
  22. ↑ Arun AK, Senthamizhselvi A, Hemamalini S, Edison ES, Korula A, Fouzia NA, et al. (December 2018). "ELANE mutations in congenital neutropenia: a single-centre study in patients of Indian origin". Journal of Clinical Pathology. 71 (12): 1046–1050. doi:10.1136/jclinpath-2018-205235. PMID   30171085. S2CID   52141047.
  23. 1 2 Dale DC (August 2017). "How I manage children with neutropenia". British Journal of Haematology. 178 (3): 351–363. doi: 10.1111/bjh.14677 . PMID   28419427.
  24. 1 2 Dale DC, Bolyard A, Marrero T, Makaryan V, Bonilla M, Link DC, et al. (December 2017). "Long-Term Effects of G-CSF Therapy in Cyclic Neutropenia". The New England Journal of Medicine. 377 (23): 2290–2292. doi:10.1056/NEJMc1709258. PMC   5777346 . PMID   29211670.
  25. ↑ Krance RA, Spruce WE, Forman SJ, Rosen RB, Hecht T, Hammond WP, Blume KG (December 1982). "Human cyclic neutropenia transferred by allogeneic bone marrow grafting". Blood. 60 (6): 1263–6. doi: 10.1182/blood.V60.6.1263.1263 . PMID   6753968.
  26. ↑ Zeidler C, Mellor-Heineke S, Klimiankou M, Skokowa J, Welte K (2015). "First Case of Leukemia in a Child Suffering from Cyclic Neutropenia with ELANE Mutation". Blood . 126 (23): 997. doi: 10.1182/blood.V126.23.997.997 .
  27. ↑ Patil VH, Hugar SM, Balikai G, Patil S (2016). "Severe congenital cyclic neutropenia: A case report". International Journal of Applied & Basic Medical Research. 6 (4): 293–296. doi: 10.4103/2229-516X.192598 . PMC   5108111 . PMID   27857902.
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