9beta-pimara-7,15-diene oxidase

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9beta-pimara-7,15-diene oxidase
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EC no. 1.14.13.144
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9beta-pimara-7,15-diene oxidase (EC 1.14.13.144, CYP99A3) is an enzyme with systematic name 9beta-pimara-7,15-diene,NADPH:oxygen 19-oxidoreductase. [1] This enzyme catalyses the following chemical reaction

9beta-pimara-7,15-diene + 3 O2 + 3 NADPH + 3 H+ 9beta-pimara-7,15-dien-19-oate + 3 NADP+ + 4 H2O (overall reaction)
(1a) 9beta-pimara-7,15-diene + O2 + NADPH + H+ 9beta-pimara-7,15-dien-19-ol + NADP+ + H2O
(1b) 9beta-pimara-7,15-dien-19-ol + O2 + NADPH + H+ 9beta-pimara-7,15-dien-19-al + NADP+ + 2 H2O
(1c) 9beta-pimara-7,15-dien-19-al + O2 + NADPH + H+ 9beta-pimara-7,15-dien-19-oate + NADP+ + H2O

9beta-pimara-7,15-diene oxidase requires cytochrome P450.

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References

  1. ↑ Wang Q, Hillwig ML, Peters RJ (January 2011). "CYP99A3: functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice". The Plant Journal. 65 (1): 87–95. doi:10.1111/j.1365-313X.2010.04408.x. PMC   3735987 . PMID   21175892.
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