Cytochrome P450, family 53, also known as CYP53, is a cytochrome P450 monooxygenase family in fungi related to hydrocarbon assimilation. [1] They are distributed in both Ascomycota and Basidiomycota, could be used as anti-fungal drug target. [2] The first gene identified in this family is the CYP53A1 from Aspergillus niger encoding the Benzoate 4-monooxygenase (bphA). [3]