Ent-kaurene oxidase

Ent-kaurene oxidase
Identifiers
EC no.	1.14.14.86
CAS no.	149565-67-3
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Ent-kaurene oxidase (EC 1.14.14.86, Formerly EC 1.14.13.78) is an enzyme with systematic name ent-kaur-16-ene,NADPH:oxygen oxidoreductase (hydroxylating). ^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

ent-kaur-16-ene + 3 NADPH + 3 H⁺ + 3 O₂${\displaystyle \rightleftharpoons }$ ent-kaur-16-en-19-oate + 3 NADP⁺ + 4 H₂O (overall reaction)

(1a) ent-kaur-16-ene + NADPH + H⁺ + O₂${\displaystyle \rightleftharpoons }$ ent-kaur-16-en-19-ol + NADP⁺ + H₂O

(1b) ent-kaur-16-en-19-ol + NADPH + H⁺ + O₂${\displaystyle \rightleftharpoons }$ ent-kaur-16-en-19-al + NADP⁺ + 2 H₂O

(1c) ent-kaur-16-en-19-al + NADPH + O₂${\displaystyle \rightleftharpoons }$ ent-kaur-16-en-19-oate + NADP⁺ + H₂O

Ent-kaurene oxidase requires cytochrome P450 and is inhibited by paclobutrazol. ^[4]

References

1. Ashman PJ, Mackenzie A, Bramley PM (November 1990). "Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi". Biochimica et Biophysica Acta (BBA) - General Subjects. 1036 (2): 151–7. doi:10.1016/0304-4165(90)90027-t. PMID   2223832.
2. Archer C, Ashman PJ, Hedden P, Bowyer JR, Bramley PM (May 1992). "Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima". Biochemical Society Transactions. 20 (2): 218S. doi:10.1042/bst020218s. PMID   1397591.
3. Helliwell CA, Poole A, Peacock WJ, Dennis ES (February 1999). "Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis". Plant Physiology. 119 (2): 507–10. doi:10.1104/pp.119.2.507. PMC   32127 . PMID   9952446.
4. Davidson, Sandra E.; Smith, Jennifer J.; Helliwell, Chris A.; Poole, Andrew T.; Reid, James B. (2004). "The Pea Gene LH Encodes ent-Kaurene Oxidase". Plant Physiology. 134 (3): 1123–1134. doi:10.1104/pp.103.032706. PMC   389936 . PMID   14988475.

External links

• Ent-kaurene+oxidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)