Steroid 15beta-monooxygenase

Steroid 15beta-monooxygenase
Identifiers
EC no.	1.14.15.8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Steroid 15beta-monooxygenase (EC 1.14.15.8, cytochrome P-450meg, cytochrome P450meg, steroid 15beta-hydroxylase, CYP106A2, BmCYP106A2) is an enzyme with systematic name progesterone,reduced-ferredoxin:oxygen oxidoreductase (15beta-hydroxylating) . ^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

progesterone + reduced ferredoxin + O₂${\displaystyle \rightleftharpoons }$ 15beta-hydroxyprogesterone + oxidized ferredoxin + H₂O

The enzyme from Bacillus megaterium hydroxylates a variety of 3-oxo-Delta4-steroids in position 15beta.

References

1. Berg A, Ingelman-Sundberg M, Gustafsson JA (June 1979). "Purification and characterization of cytochrome P-450meg". The Journal of Biological Chemistry. 254 (12): 5264–71. doi: 10.1016/S0021-9258(18)50589-5 . PMID   109432.
2. Berg A, Gustafsson JA, Ingelman-Sundberg M (May 1976). "Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium". The Journal of Biological Chemistry. 251 (9): 2831–8. doi: 10.1016/S0021-9258(17)33564-0 . PMID   177422.
3. Lisurek M, Kang MJ, Hartmann RW, Bernhardt R (June 2004). "Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry". Biochemical and Biophysical Research Communications. 319 (2): 677–82. Bibcode:2004BBRC..319..677L. doi:10.1016/j.bbrc.2004.05.037. PMID   15178459.
4. Goñi G, Zöllner A, Lisurek M, Velázquez-Campoy A, Pinto S, Gómez-Moreno C, Hannemann F, Bernhardt R, Medina M (November 2009). "Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368". Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1794 (11): 1635–42. doi:10.1016/j.bbapap.2009.07.012. PMID   19635596.
5. Lisurek M, Simgen B, Antes I, Bernhardt R (June 2008). "Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation". ChemBioChem. 9 (9): 1439–49. doi:10.1002/cbic.200700670. PMID   18481342. S2CID   10667895.

External links

• Steroid+15beta-monooxygenase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)