Flavonoid 3',5'-hydroxylase

Flavonoid 3',5'-hydroxylase
Identifiers
EC no.	1.14.14.81
CAS no.	94047-23-1
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Flavonoid 3',5'-hydroxylase (EC 1.14.14.81 was wrongly classified as EC 1.14.13.88 in the past) is an enzyme with systematic name flavanone,NADPH:oxygen oxidoreductase. ^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

flavanone + 2 NADPH + 2 H⁺ + 2 O₂${\displaystyle \rightleftharpoons }$ 3',5'-dihydroxyflavanone + 2 NADP⁺ + 2 H₂O (overall reaction)

(1a) flavanone + NADPH + H⁺ + O₂${\displaystyle \rightleftharpoons }$ 3'-hydroxyflavanone + NADP⁺ + H₂O

(1b) 3'-hydroxyflavanone + NADPH + H⁺ + O₂${\displaystyle \rightleftharpoons }$ 3',5'-dihydroxyflavanone + NADP⁺ + H₂O

Flavonoid 3',5'-hydroxylase is a heme-thiolate protein (P-450).

References

1. Menting J, Scopes RK, Stevenson TW (October 1994). "Characterization of Flavonoid 3[prime],5[prime]-Hydroxylase in Microsomal Membrane Fraction of Petunia hybrida Flowers". Plant Physiology. 106 (2): 633–642. doi:10.1104/pp.106.2.633. PMC   159570 . PMID   12232356.
2. Shimada Y, Nakano-Shimada R, Ohbayashi M, Okinaka Y, Kiyokawa S, Kikuchi Y (November 1999). "Expression of chimeric P450 genes encoding flavonoid-3', 5'-hydroxylase in transgenic tobacco and petunia plants(1)". FEBS Letters. 461 (3): 241–5. doi: 10.1016/s0014-5793(99)01425-8 . PMID   10567704.
3. de Vetten N, ter Horst J, van Schaik HP, de Boer A, Mol J, Koes R (January 1999). "A cytochrome b5 is required for full activity of flavonoid 3', 5'-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors". Proceedings of the National Academy of Sciences of the United States of America. 96 (2): 778–83. doi: 10.1073/pnas.96.2.778 . PMC   15213 . PMID   9892710.

External links

• Flavanoid+3',5'-hydroxylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)