Proteases in angiogenesis

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Angiogenesis is the process of forming new blood vessels from existing blood vessels, formed in vasculogenesis. It is a highly complex process involving extensive interplay between cells, soluble factors, and the extracellular matrix (ECM). Angiogenesis is critical during normal physiological development, but it also occurs in adults during inflammation, wound healing, ischemia, and in pathological conditions such as rheumatoid arthritis, hemangioma, and tumor growth. [1] [2] Proteolysis has been indicated as one of the first and most sustained activities involved in the formation of new blood vessels. Numerous proteases including matrix metalloproteinases (MMPs), a disintegrin and metalloproteinase domain (ADAM), a disintegrin and metalloproteinase domain with throbospondin motifs (ADAMTS), and cysteine and serine proteases are involved in angiogenesis. This article focuses on the important and diverse roles that these proteases play in the regulation of angiogenesis.

Contents

MMPs

Matrix metalloproteinases (MMPs) are a large multigene family of zinc-dependent endopeptidases. The collective MMP family is capable of degrading all known ECM macromolecules. MMP activity is regulated at the level of transcription, post-translationally by proteolytic cleavage, and by endogenous inhibitors known as tissue inhibitors of metalloproteinases (TIMPs). The role of matrix metalloproteinases and TIMPs in several pathological conditions including angiogenesis, tumor growth, and metastasis has been investigated and very well described.

Matrix metalloproteinases contain five conserved domains/sequence motifs:

  1. Signal peptide sequence, which directs the enzyme into the rough endoplasmic reticulum during synthesis
  2. Propeptide domain, which is cleaved to activate the enzyme
  3. Catalytic domain, which contains the conserved Zn2+ binding region and mediates enzyme activity
  4. Hemopexin domain, which provides the substrate specificity
  5. Small hinge region, which allows the hemopexin domain to bring the substrate to the active core of the catalytic domain

There is also a subfamily of the matrix metalloproteinases, the membrane-type MMPs (MT-MMPs) which contain an additional transmembrane domain and a short cytoplasmic domain. After activation of MMPs by removal of the propeptide domain, their activity is regulated by TIMPs. TIMPs specifically and reversibly inhibit the activity of MMPs. So far there have been identified four members of the family, TIMP1–4. All TIMPs contain twelve conserved cystein residues, which form six disulfide bonds. The C-terminal domains of TIMPs are highly variable and confer their specificity towards preferred MMP targets. [3] [4]

ADAM/ADAMTS

ADAM17 PBB Protein ADAM17 image.jpg
ADAM17

ADAMs comprise a family of integral membrane as well as secreted glycoproteins which are related to snake venom metalloproteinases and MMPs. Like MMPs, ADAMs are composed of multiple conserved domains. They contain propeptide, metalloproteinase, disintegrin-like, cystein-rich, and epidermal growth factor like domains, although variations in domain composition have been observed in non-animal organisms. [5] Membrane anchored ADAMs contain a transmembrane and cytoplasmic domain. The domains contained within the ADAMs family have been characterized, uncovering their functional and structural roles. [6] ADAMs contain a consensus sequence which has three histidine residues that bind to the catalytically essential zinc ion. The propeptide is removed through cleavage by a furin type protease yielding the active enzyme. The propeptide of most MMPs is cleavable by proteases such as trypsin, plasmin, chymotrypsin and other MMPs. [7] ADAMs participate in a wide variety of cell surface remodeling processes, including ectodomain shedding, regulation of growth factor availability and mediating cell-matrix interactions. ADAM17 and ADAM15 have recently been identified in endothelial cells (EC). [8]

ADAMTS are a subfamily of ADAM related metalloproteinases that contain at least one thrombospondin type I sequence repeat motif (TSR). They are secreted proteins; and the TSR facilitates their localization to the ECM placing it in close proximity to their substrates. Functionally, ADAMTS can be divided into three groups: procollagen aminopeptidase, aggrecanase, and ADAMTS13 which cleaves von Willebrand factor. Unlike with MMPs, TIMPs are more selective in their ability to inhibit ADAMs and ADAMTSs. TIMP3 is able to inhibit ADAM17 and 12 as well as ADAMTS4 and 5. ADAM8 and ADAM9 are not susceptible to inhibition by TIMPs.

Other proteolytic enzymes

Many additional classes of enzymes have been identified that facilitate angiogenesis. They include serine, aspartic, and cysteine-type proteases. A highly characterized example of the serine protease family is the plasminogen activator-plasmin system, which has been shown to be involved in vascular remodelling . Tissue plasminogen activator (tPA), and urokinase plasminogen activator (urokinase, uPA) are serine proteases which cleave and activate plasminogen. The activated form of plasminogen, plasmin, is a wide-ranging protease capable of acting on various ECM components including fibrin, collagens, laminin, fibronectin, and proteoglycans. [9] Additionally, plasmin also is able to activate various other MMPs.

In humans, the group of cathepsin cysteine proteases or cysteine cathepsins comprises 11 family members, cathepsins B, C, F, H, L1, L2, K, O, S, W, and X/Z. [10] Cysteine cathepsins are synthesized as inactive zymogens and activated by proteolytic removal of their propeptide. These enzymes are primarily localized in lysosomes and function in terminal protein degradation and processing. Cathepsins also can be secreted by cells, associate with the cell surface, and degrade the ECM. A study of all 11 members of the cathepsin family highlights their importance in tumorigenesis and tumor associated angiogenesis. [11] Examination of cathepsin activity by using chemical probes and in vivo imaging techniques demonstrated an increase in cathepsin activity in the angiogenic blood vessels and invasive fronts of carcinoma in the RIP-Tag2 transgenic mouse model of pancreatic islet tumor genesis.

Aminopeptidases function as exopeptidases which remove amino acids from the amino-terminus of proteins. Aminopeptidase N (CD13/APN) is highly expressed on the endothelium of growing vessels. [12] Inhibitors of CD13/APN dramatically impair tumor growth.

Ectodomain shedding

Diagram of an ectodomain shedding ADAM metalloproteinase. Ectodomain shedding en.svg
Diagram of an ectodomain shedding ADAM metalloproteinase.

It has become clear in the past years that ectodomain shedding is an initial step for the activation of specific receptors such as Notch, ErbB-4 and the angiopoietin receptor Tie-1. Notch-1 signaling is essential for endothelial differentiation, and tumor angiogenesis, while the angiopoietin receptor Tie-1 facilitates embryonic blood vessel formation. [13] [14] Upon binding of their ligands, Notch-1 and Tie-1 undergo proteolytic cleavage of the ectodomains by ADAM17 and ADAM10. This cleavage frees the cytoplasmic fragment for cellular signaling. In the case of Notch-1, it transfers to the nucleus.

Many cytokines and growth factors are synthesized as membrane-bound proforms which undergo proteolytic shedding for activation. The ephrins EPH receptor A2 and A3 are shed by ADAM10, creating cleaved soluble Eph receptors, which inhibit tumor angiogenesis in mice. [15] Additional examples are the proteolytic shedding of soluble E-selectin, [16] shedding of urokinase receptor (uPAR) by MMP-12 creating soluble uPAR which has chemotactic properties for leukocytes and progenitor cells, and the shedding of interleukin-6 receptors by ADAM10 and ADAM17 which facilitates interleukin-6 signaling in endothelial cells. [17] Semaphorin 4D is cleaved from its membrane-bound form by MT1-MMP (MMP-14) in tumor cells; it then interacts with plexin B1 on endothelial cells, promoting pro-angiogenic chemotaxis. [18] Shedding of a membrane-anchored cytokine or growth factor by ADAM proteinases may be relevant for various signal transduction events. Alternatively, shedding may be required for the ligand to diffuse to distant receptors. Shedding may be required for the down regulation of signals by removing signaling ligands, or cleavage and release of receptors. Release of the receptor may also generate soluble receptors which act as decoys by sequestering ligands. These findings indicate that ectodomain shedding is a ubiquitous process facilitating a wide variety of cellular events involved in angiogenesis. Because potent biological modifiers are generated, it is likely controlled by highly regulated mechanism. Along with ADAMs and MT-MMPs, membrane-bound serine proteases also may play a role in ectodomain shedding.

Proteolytic degradation of the extracellular matrix (ECM)

Illustration depicting extracellular matrix in relation to epithelium, endothelium and connective tissue. Extracellular Matrix.png
Illustration depicting extracellular matrix in relation to epithelium, endothelium and connective tissue.

The formation of capillaries from pre-existing blood vessels requires the remodeling of both the peicapillary membrane of the parent venule, as well as the local and distal ECM. At the onset of angiogenesis endothelial cells (EC) must remodel three different barriers in order to migrate and invade the target tissue. First is the basement membrane between the endothelium and vascular smooth muscle cells or pericytes, followed by the fibrin gel formed from fibrinogen that is leaked from the vasculature, and finally the extracellular matrix in the target tissue. The vascular basement membrane is composed of type IV collagen, type XV collagen, type XVIII collagen, laminins, entactin, heparan sulfate proteoglycans, perlecan, and osteonectin. All of these components of the basement membrane are substrates for MMP-2, 3, 7, and 9, among others. Inhibitors of MMP activity have spotlighted the importance of these proteins in controlling angiogenesis. Recently, it has been discovered that small interfering RNA (siRNA) mediated target RNA degradation of urokinase receptor and MMP-9 inhibits the formation of capillary like structures in both in vitro and in vivo models of angiogenesis. [19] After working their way through the basement membrane, EC must invade through a dense fibrin gel which is polymerized from fibrinogen derived from the vascular bed. [20] Plasmin, an effective fibrinolysin produced by tPA or uPA, was thought to be essential in this process, but plasminogen deficient mice do not display major defects of neovascularization in fibrin rich tissues. [21] These findings highlight the diverse amount of proteolytic enzymes ECs use to remodel the ECM. For example, MMP-3, 7, 8, 12 and 13 can cleave fibrinogen. [22]

MMP activity is one of the earliest and most sustained processes that take place during angiogenesis. By studying the transition from an avascular to a vascular tumor Fang et al. were able to identify the key role of MMP-2 in angiogenesis. MMP-2 expression and activity was increased in angiogenic tumors as compared with avascular tumors, and the addition of antisense oligonucleotides targeting MMP-2 inhibits the initiation of angiogenesis maintaining the avascular phenotype. This data along with other reports suggest that MMP activity is necessary to initiate the earliest stages of angiogenesis and tumor development. The creation of MMP deficient mice has provided important insight into the role of MMPs in the regulation of angiogenesis. For example, MMP-2 knockout mice develop normally but display significant inhibition of corneal angiogenesis. [23]

Proteolytic fragments as regulators of angiogenesis

Numerous proteolytic fragments or domains of ECM proteins have been reported to exert positive or negative activity on angiogenesis. Native proteins which contain such domains with regulatory activity are normally inactive, most likely because they are cryptic segments hidden in the native protein structure. Angiostatin is a 38 kDa plasminogen fragment with angiogenesis inhibitor activity. Angiostatin fragments contain kringle domains which exert their inhibitory activity at several different levels; they inhibit endothelial cell migration and proliferation, increase apoptosis, and modulate the activity of focal adhesion kinase (FAK). Endostatin is a 20 kDa fragment of collagen XVIII. The major role of endostatin is in its ability to potently inhibit endothelial cell migration and induce apoptosis. [24] These effects are mediated by interacting and interfering with various angiogenic related proteins such as integrins and serine/threonine-specific protein kinases. Numerous studies have demonstrated that tropoelastin, the soluble precursor of elastin, or proteolytic elastin fragments have diverse biological properties. Nackman et al. demonstrated that elastase generated elastin fragments mediate several characteristic features of aneurysmal disease which correlated to angiogenesis. Osteonectin is a metal binding glycoprotein produced by many cell types including ECs. Lastly, endorepellin is a recently described inhibitor of angiogenesis derived from the carboxy terminus of perlecan. [25] Nanomolar concentrations of endorepellin inhibits EC migration and angiogenesis in different in vitro and in vivo models by blocking EC adhesion to various substrate such as fibronectin and type I collagen.

Endogenous inhibitors or activators generated by proteolytic degradation of larger proteins mostly from the ECM have proven to contribute to the regulation of tumor growth and angiogenesis. This article mentions only a small fraction of the known proteolytic fragments which alter EC behavior and function during angiogenesis. This abundance has garnered increased attention because of their potential for anti-angiogenic and anti-cancer therapies.

Proteolytic activation of growth factors

Proteases not only modulate cell-matrix interactions but also can control the onset and progression of angiogenesis by activating angiogenic growth factors and cytokines. Hepatocyte growth factor (HGF), an angiogenesis promoting growth factor, is activated by HGF activation factor, a serine protease related to plasminogen. [26] Several growth factors such as basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) are trapped in the ECM by various proteoglycans. The proteolytic degradation of these proteoglycans liberates the growth factors allowing them to reach their receptors and influence cellular behavior. Growth factors that indirectly affect angiogenesis are also targets of proteolytic activation. For example, plasminogen activators drive the activation of latent transforming growth factor beta (TGF-β) from bone ECM and thus modulate angiogenesis in bone. [27]

Proteases not only have the ability change the availability of growth factors, but can also modify their properties. This ability was shown for VEGF165 that is cleaved by MMP-3 or MMP-9 to a smaller molecule with properties similar to VEGF121. [28] These two isoforms of VEGF have very different properties. VEGF165 induces a regular vessel pattern during tumor neovascularization. VEGF121 and the truncated VEGF165, in contrast, cause irregular patterns of neovascularization, most likely due to their inability to bind heparan sulfates, wherefore they do not provide any spatial information that is buried in the ECM. Another important factor in angiogenesis, stromal cell-derived factor-1 (SDF-1), is also modified by the aminodipeptidase dipeptidyl peptidase-4 (DPP4). Cleavage of SDF-1 reduces it heparan sulfate affinity and interactions with its receptor CXCR4 are reduced. [29] The ADAM family of proteases is receiving increased attention for their ability to alter the balance between pro-and anti-angiogenic factors. ADAM17 is able to release active tumor necrosis factor-alpha (TNFα) and heparin-binding EGF-like growth factor (HB-EGF) from their membrane bound precursors which can indirectly affect angiogenesis. [30]

Proteases as inhibitors of angiogenesis

Proteases not only facilitate angiogenesis, but they also have the ability to put the brakes on the process. One example of this is the processing of angiogenesis inhibitors by MMPs. As previously described, MMPs have been shown to cleave plasminogen and collagen XVIII into the endogenous angiogenesis inhibitors angiostatin and endostatin. MMP-2 itself possesses anti-angiogenic properties that are independent of its catalytic domain. Interactions between integrin αvβ3 and MMP-2 on the EC cell surface may be necessary for MMP-2 activity during angiogenesis. The hemopexin like domain in the carboxy terminus of MMP-2 is able to block this interaction of active MMP-2 and integrin αvβ3 on the EC surface which lead to inhibition of MMP-2 activity. [31]

During angiogenesis ADAM15 is preferentially expressed on EC. ADAM15 is able to interact with integrins αvβ3 and α5β1 through its disintegrin domain via an RGD (arginine-glycine-aspartic acid) motif. Most disintegrins contain this conserved RGD motif, but ADAM15 is the only member of the ADAM family to contain this motif. A recombinant disintegrin domain of human ADAM15 inhibits a variety of EC functions in vitro including proliferation, adhesion, migration, and capillary formation. [32] Overexpression of ADAM15 disintegrin domain resulted in inhibition of angiogenesis, tumor growth, and metastasis. On the other hand, it has not been shown whether full length ADAM15 plays an inhibitory role in vivo. ADAMTS1 and ADAMTS8 inhibit angiogenesis in vitro in two functional angiogenesis assays. Both enzymes inhibit bFGF induced vascularization in the corneal pocket assay and inhibit VEGF induced angiogenesis in the chorioallantoic membrane assay. [33] All together, these data indicate that proteases can function as both positive and negative regulators of angiogenesis.

Proteolysis and cell migration

Angiogenesis requires the migration and invasive growth of cells. This is facilitated by a balanced interplay between detachment and formation of cell adhesions which enable the cell to crawl forward through the ECM. [34] The cell uses limited proteolytic activity at sites of individual focal adhesions via the formation of multiprotein complexes. Multiprotein complexes are localized in lipid rafts on the cell surface, where membrane bound proteases are often incorporated. For example, leukocytes complex urokinase (uPA), urokinase receptor (uPAR), and integrins which participate in cell adhesion and invasion. [35] In these complexes, uPAR acts as an organizing center forming noncovalent complexes with integrins, LRP-like proteins, and urokinase. Similar complexes also are found on ECs.

Uncontrolled proteolysis of the ECM

The proteolytic activities that take place during angiogenesis require precise spatial and temporal regulation. If not for this control excessive proteolysis could lead to damage of the tissue and the loss of anchorage points for migrating cells. This is illustrated by mice which are deficient for plasminogen activator inhibitor-1 (PAI-1). [36] [37] PAI-1 inhibits plasminogen activators and thus plasmin activation; therefore it could be assumed that PAI-1 deficiency would increase angiogenesis and tumor growth. Unexpectedly, when PAI-1 deficient mice were challenged with cancer cells on a collagenous matrix, angiogenesis and vascular stabilization was inhibited, hampering tumor growth. This finding was credited to the protective properties PAI-1 imparts against excessive degradation of the surrounding ECM by plasmin. Without this protection the footholds used by endothelial cells to migrate and form capillary structures are destroyed. Uncontrolled proteolysis also is attributed to the disruption of vascular development and premature deaths in murine embryos deficient of the inhibitor reversion-inducing-cysteine-rich protein with kazal motifs (RECK). This is most likely due to uncontrolled MMP activity, because a partial rescue was obtained by simultaneously knocking out RECK and MMP-2. [38]

Proteases involved in the recruitment of bone marrow derived cells during angiogenesis

Leukocytes and endothelial progenitor cells (EPCs) contribute to the initiation and guidance of new blood vessels. [39] Monocytes produce a variety of pro-angiogenic factors. There is also a population of CD34 positive cells that can express endothelial associated proteins, such as VE-cadherin and kinase insert domain receptor (KDR, VEGF receptor 2) which aid in influencing the progression of angiogenesis. [40] The absence or dysfunction of these cells is implicated in impaired vascularization in cardiac and diabetes patients. [41] MMP-9 plays a key role in mobilizing EPCs from the bone marrow. Heissig et al. have proposed a mechanism for how MMP-9 facilitates the availability of EPCs for angiogenesis. First, circulating VEGF induces MMP-9 expression in the bone marrow, MMP-9 then is able to cleave and release c-kit ligand. Activated c-kit is then able to recruit hematopoietic, endothelial and mast cell progenitor cells, these cells are then accumulated in the angiogenic area and produce large amounts of VEGF tipping the scales in favor of angiogenesis. [42]

MMP-9 is not the only protease shown to be involved in EPC enhanced angiogenesis. Cathepsin L1 is active at neutral pH by associating with a p41 splice variant of the MHC class II-associated invariant chain which is strongly expressed in EPCs. [43] This ability to stay active at neutral pH may facilitate EPC invasion, remodeling of matrix collagens and gelatin, and neovascularization. Knock out of cathepsin L1 in mice exhibited impaired blood flow restoration in ischemic limbs, indicating impaired neovascularization. Neovascularization also is impaired in mice treated with bone marrow derived cells deficient of cathepsin L1 as compared with wild type cells. The target by which cathepsin L1 stimulates angiogenesis is not yet identified.

Maturation of newly formed blood vessels via proteases

MT1-MMP (MMP-14) PBB Protein MMP14 image.jpg
MT1-MMP (MMP-14)

It has been well established that smooth muscle-like pericytes play an important role in stabilizing newly formed blood vessels. Pericytes present in the stroma of tumors of breast cancer patients express MMP-9. [44] Animal models deficient of MMP-9 display disturbed recruitment of pericytes. [45] The inability to recruit pericytes severely affects the stability of vessels and the degree of vascularization of neuroblastomas. Aminopeptidase A also may be involved in pericyte recruitment due to its increased expression by activated pericytes in various pathological conditions associated with angiogenesis. [46] The mechanism by which this protease facilitates vessel maturation has not yet been determined. Angiogenesis requires a fine balance between proteolytic activity and proteinase inhibition. Pericytes secrete TIMP-3 which inhibits MT1-MMP dependent MMP-2 activation on endothelial cell, thus facilitating stabilization of newly formed microvessels. Co-cultures consisting of pericytes and endothelial cells induce the expression of TIMP-3 by pericytes, while endothelial cells produce TIMP-2. [47] Together, these inhibitors stabilize the vasculature by inhibiting a variety of MMPs, ADAMs, and VEGF receptor 2.

Immature vessels remain dependent on continuous exposure the angiogenic growth factors without pericyte coverage. [48] As the reservoir of growth factors is removed the endothelial cells do not survive, and undergo caspases induced apoptosis, while other proteases participate in the degradation and removal of the remaining cell debris.

Perspective

Proteases play numerous roles in angiogenesis, both in development and especially in pathological conditions. Because they are important regulators of tissue degradation and cell migration, it is expected that their inhibition would be beneficial for inhibiting tumor growth and vascularization. Promising results have been observed in animal studies, but clinical trials have failed to demonstrate similar results and are often accompanied by unacceptable side effects. [49] This has influenced continued research which has identified new families of proteases, such as ADAM, ADAMTS, and MT-MMPs. Perhaps more significantly, a new paradigm has emerged for proteases being essential for modulating growth factors and cytokines, generating biologically active fragments from the matrix, facilitating recruitment of bone marrow derived cells, and stabilization of mature blood vessels. Better understanding of the various activities of proteases and their inhibitors will aid in more tailor made treatments for numerous disorders.

Related Research Articles

<span class="mw-page-title-main">Angiogenesis</span> Blood vessel formation, when new vessels emerge from existing vessels

Angiogenesis is the physiological process through which new blood vessels form from pre-existing vessels, formed in the earlier stage of vasculogenesis. Angiogenesis continues the growth of the vasculature mainly by processes of sprouting and splitting, but processes such as coalescent angiogenesis, vessel elongation and vessel cooption also play a role. Vasculogenesis is the embryonic formation of endothelial cells from mesoderm cell precursors, and from neovascularization, although discussions are not always precise. The first vessels in the developing embryo form through vasculogenesis, after which angiogenesis is responsible for most, if not all, blood vessel growth during development and in disease.

Angiostatin is a naturally occurring protein found in several animal species, including humans. It is an endogenous angiogenesis inhibitor. Clinical trials have been undertaken for its use in anticancer therapy.

<span class="mw-page-title-main">Urokinase</span> Human protein

Urokinase, also known as urokinase-type plasminogen activator (uPA), is a serine protease present in humans and other animals. The human urokinase protein was discovered, but not named, by McFarlane and Pilling in 1947. Urokinase was originally isolated from human urine, and it is also present in the blood and in the extracellular matrix of many tissues. The primary physiological substrate of this enzyme is plasminogen, which is an inactive form (zymogen) of the serine protease plasmin. Activation of plasmin triggers a proteolytic cascade that, depending on the physiological environment, participates in thrombolysis or extracellular matrix degradation. This cascade had been involved in vascular diseases and cancer progression.

An angiogenesis inhibitor is a substance that inhibits the growth of new blood vessels (angiogenesis). Some angiogenesis inhibitors are endogenous and a normal part of the body's control and others are obtained exogenously through pharmaceutical drugs or diet.

<span class="mw-page-title-main">Endostatin</span>

Endostatin is a naturally occurring, 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin.

<span class="mw-page-title-main">Plasminogen activator</span> Type of protein

Plasminogen activators are serine proteases that catalyze the activation of plasmin via proteolytic cleavage of its zymogen form plasminogen. Plasmin is an important factor in fibrinolysis, the breakdown of fibrin polymers formed during blood clotting. There are two main plasminogen activators: urokinase (uPA) and tissue plasminogen activator (tPA). Tissue plasminogen activators are used to treat medical conditions related to blood clotting including embolic or thrombotic stroke, myocardial infarction, and pulmonary embolism.

Intravasation is the invasion of cancer cells through the basement membrane into a blood or lymphatic vessel. Intravasation is one of several carcinogenic events that initiate the escape of cancerous cells from their primary sites. Other mechanisms include invasion through basement membranes, extravasation, and colonization of distant metastatic sites. Cancer cell chemotaxis also relies on this migratory behavior to arrive at a secondary destination designated for cancer cell colonization.

<span class="mw-page-title-main">Angiopoietin</span> Protein family

Angiopoietin is part of a family of vascular growth factors that play a role in embryonic and postnatal angiogenesis. Angiopoietin signaling most directly corresponds with angiogenesis, the process by which new arteries and veins form from preexisting blood vessels. Angiogenesis proceeds through sprouting, endothelial cell migration, proliferation, and vessel destabilization and stabilization. They are responsible for assembling and disassembling the endothelial lining of blood vessels. Angiopoietin cytokines are involved with controlling microvascular permeability, vasodilation, and vasoconstriction by signaling smooth muscle cells surrounding vessels. There are now four identified angiopoietins: ANGPT1, ANGPT2, ANGPTL3, ANGPT4.

<span class="mw-page-title-main">Endoglin</span> Protein-coding gene in the species Homo sapiens

Endoglin (ENG) is a type I membrane glycoprotein located on cell surfaces and is part of the TGF beta receptor complex. It is also commonly referred to as CD105, END, FLJ41744, HHT1, ORW and ORW1. It has a crucial role in angiogenesis, therefore, making it an important protein for tumor growth, survival and metastasis of cancer cells to other locations in the body.

<span class="mw-page-title-main">Thrombospondin 1</span> Protein-coding gene in the species Homo sapiens

Thrombospondin 1, abbreviated as THBS1, is a protein that in humans is encoded by the THBS1 gene.

<span class="mw-page-title-main">Neuropilin</span> Protein receptor active in neurons

Neuropilin is a protein receptor active in neurons.

<span class="mw-page-title-main">MMP9</span> Protein-coding gene in the species Homo sapiens

Matrix metalloproteinase-9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is a matrixin, a class of enzymes that belong to the zinc-metalloproteinases family involved in the degradation of the extracellular matrix. In humans the MMP9 gene encodes for a signal peptide, a propeptide, a catalytic domain with inserted three repeats of fibronectin type II domain followed by a C-terminal hemopexin-like domain.

<span class="mw-page-title-main">MMP2</span> Protein-coding gene in the species Homo sapiens

72 kDa type IV collagenase also known as matrix metalloproteinase-2 (MMP-2) and gelatinase A is an enzyme that in humans is encoded by the MMP2 gene. The MMP2 gene is located on chromosome 16 at position 12.2.

<span class="mw-page-title-main">TIMP2</span> Protein-coding gene in the species Homo sapiens

Tissue inhibitor of metalloproteinases 2 (TIMP2) is a gene and a corresponding protein. The gene is a member of the TIMP gene family. The protein is thought to be a metastasis suppressor.

<span class="mw-page-title-main">MMP7</span> Protein-coding gene in humans

Matrilysin also known as matrix metalloproteinase-7 (MMP-7), pump-1 protease (PUMP-1), or uterine metalloproteinase is an enzyme in humans that is encoded by the MMP7 gene. The enzyme has also been known as matrin, putative metalloproteinase-1, matrix metalloproteinase pump 1, PUMP-1 proteinase, PUMP, metalloproteinase pump-1, putative metalloproteinase, MMP). Human MMP-7 has a molecular weight around 30 kDa.

<span class="mw-page-title-main">PEDF</span> Protein-coding gene in the species Homo sapiens

Pigment epithelium-derived factor (PEDF) also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functions. Found in vertebrates, this 50 kDa protein is being researched as a therapeutic candidate for treatment of such conditions as choroidal neovascularization, heart disease, and cancer. In humans, pigment epithelium-derived factor is encoded by the SERPINF1 gene.

<span class="mw-page-title-main">ADAMTS1</span> Protein-coding gene in the species Homo sapiens

A disintegrin and metalloproteinase with thrombospondin motifs 1 is an enzyme that in humans is encoded by the ADAMTS1 gene.

<span class="mw-page-title-main">Photoaging</span> Ultraviolet light-induced biological development over time

Photoaging or photoageing is a term used for the characteristic changes to skin induced by chronic UVA and UVB exposure. Tretinoin is the best studied retinoid in the treatment of photoaging.

<span class="mw-page-title-main">Metastatic breast cancer</span> Type of cancer

Metastatic breast cancer, also referred to as metastases, advanced breast cancer, secondary tumors, secondaries or stage IV breast cancer, is a stage of breast cancer where the breast cancer cells have spread to distant sites beyond the axillary lymph nodes. There is no cure for metastatic breast cancer; there is no stage after IV.

Tumor-associated macrophages (TAMs) are a class of immune cells present in high numbers in the microenvironment of solid tumors. They are heavily involved in cancer-related inflammation. Macrophages are known to originate from bone marrow-derived blood monocytes or yolk sac progenitors, but the exact origin of TAMs in human tumors remains to be elucidated. The composition of monocyte-derived macrophages and tissue-resident macrophages in the tumor microenvironment depends on the tumor type, stage, size, and location, thus it has been proposed that TAM identity and heterogeneity is the outcome of interactions between tumor-derived, tissue-specific, and developmental signals.

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