Apusomonad

Apusomonad
Podomonas kaiyoae SEM image. AF: anterior flagellum. PF: posterior flagellum. T: tusk. Brackets: acronemes. Arrows and arrowheads: pseudopodium. Scale bar = 5 μm.
Scientific classification
Domain:	Eukaryota
Clade:	Amorphea
Clade:	Obazoa
Class:	Thecomonadea Cavalier-Smith, 1993 emend. 2013 [1]
Order:	Apusomonadida Karpov & Mylnikov, 1989 [2]
Family:	Apusomonadidae Karpov & Mylnikov, 1989 [2]
Genera
See text
Diversity
28 species

The apusomonads (family Apusomonadidae) are a group of protozoan zooflagellates that glide on surfaces, and mostly consume prokaryotes. They are of particular evolutionary interest because they appear to be the sister group to the Opisthokonts, the clade that includes both animals and fungi. Together with the Breviatea, these form the Obazoa clade. ^{[3] [4] [5]}

Characteristics

Apusomonads are small gliding heterotrophic biflagellates (i.e. with two flagella) that possess a proboscis, formed partly or entirely by the anterior flagellum surrounded by a membranous sleeve. There is a pellicle under the dorsal cell membrane that extends into the proboscis sleeve and into a skirt that covers the sides of the cell. Apusomonads present two different cell plans: ^[6]

• Derived cell plan, represented by Apusomonas , with a round cell body and a mastigophore, a projection of the cell containing both basal bodies at its end. ^[6]
• "Amastigomonas-like" cell plan, with an oval or oblong cell that generally forms pseudopodia from the ventral surface, with no mastigophore, and the proboscis comprising solely the flagellum and the sleeve. These characteristics are considered 'primitive' or 'ancestral' in comparison with Apusomonas . Organisms with this body plan, although historically assigned to the same genus Amastigomonas , are a paraphyletic group from which Apusomonas has evolved. ^{[6] [7]}

Evolution

External relationships

The apusomonads are the sister group to Opisthokonta, the lineage that includes animals, fungi and an array of related protists. Because of this, apusomonads occupy an important phylogenetic position to understand eukaryotic evolution. They retain ancestral characteristics, such as the biflagellate body plan, which in opisthokonts evolves into a uniflagellate plan. ^[7]

Apusomonads are vital to understanding multicellularity. Genes involved in multicellularity have been found in the apusomonad Thecamonas , ^[8] such as adhesion proteins, calcium-signaling genes and types of sodium channels characteristic of animals. ^[6] The genome of the strain " Amastigomonas sp." presents the integrin-mediated adhesion machinery, the primary cell-matrix adhesion mechanism seen in Metazoa (animals). ^[9]

Amorphea	Amoebozoa Obazoa Breviatea Apusomonadida Opisthokonta Holozoa Ichthyosporea Pluriformea Tunicaraptor Filozoa Filasterea Choanozoa Choanoflagellata Metazoa Holomycota Fungi Nucleariae

Internal relationships

Apusomonads are a poorly and narrowly studied group. ^[6] Currently, the diversity of described apusomonads consists of the round Apusomonas and a wide array of " Amastigomonas -type" organisms that have been reclassified into the genera Thecamonas , Manchomonas , Podomonas , Multimonas , Chelonemonas and, most recently, Catacumbia , Cavaliersmithia , Karpovia , Mylnikovia and Singekia . The relationships between these genera are depicted by the cladogram below. ^[7]

Apusomonadida	Apusomonadinae Apusomonas Manchomonas Thecamonadinae Karpovia Singekia Chelonemonas Thecamonas Podomonas Multimonas Mylnikovia Cavaliersmithia Catacumbia	" Amastigomonas -like" organisms

Taxonomy

History

Apusomonads were first described in 1989 as one family Apusomonadidae inside the monotypic order Apusomonadida, as a group of flagellates containing the genera Apusomonas and Amastigomonas . ^[2] Later, British protozoologist Thomas Cavalier-Smith classified them within the monotypic class Thecomonadea as part of the paraphyletic phylum Apusozoa. ^[1] Modern cladistic approaches to eukaryotic classification refer to apusomonads by their order-level name alone. ^{[7] [10]}

Classification

There are 10 recognized genera, as well as the " Amastigomonas -like" archetype that includes primitive forms not yet transferred to new genera. ^[7]

• Amastigomonas de Saedeleer 1931
  • A. caudataMylnikov 1989 [Amastigomonas borokensisHamar 1979]
  • A. debruyneide Saedeleer 1931
  • A. marisrubriMylnikov & Mylnikov 2012
• Catacumbia Torruella, Galindo et al. 2022 ^[7]
  • C. lutetiensisTorruella, Galindo et al. 2022
• Cavaliersmithia Torruella, Galindo et al. 2022
  • C. chaoaeTorruella, Galindo et al. 2022
• Multimonas Cavalier-Smith 2010
  • M. koreensisHeiss, Lee, Ishida & Simpson, 2015
  • M. marina(Mylnikov 1989) Cavalier-Smith 2010 [Cercomonas marinaMylnikov 1989; Amastigomonas marina(Mylnikov 1989) Mylnikov 1999]
  • M. mediaCavalier-Smith 2010
• Mylnikovia Torruella, Galindo et al. 2022
  • M. oxoniensis(Cavalier-Smith 2010) Torruella, Galindo et al. 2022 [Thecamonas oxoniensisCavalier-Smith 2010]
• Podomonas Cavalier-Smith 2010
  • P. capensisCavalier-Smith 2010
  • P. gigantea(Mylnikov 1999) [Amastigomonas giganteaMylnikov 1999]
  • P. griebenis(Mylnikov 1999) [Amastigomonas griebenisMylnikov 1999]
  • P. kaiyoaeYabuki in Yabuki, Tame & Mizuno 2022 ^[11]
  • P. klosteris(Arndt & Mylnikov 1999) Cavalier-Smith 2010 [Amastigomonas klosterisArndt & Mylnikov 1999]
  • P. magnaCavalier-Smith 2010
• Apusomonadinae Cavalier-Smith 2010 ^[3]
  • Apusomonas Alexeieff 1924 [ Rostromonas Karpoff & Zhukov 1980]
    • A. australiensisEkelund & Patterson 1997
    • A. proboscideaAlexeieff 1924 [Rostromonas applanataKarpoff & Zhukov 1980]
  • Manchomonas Cavalier-Smith 2010
    • M. bermudensis(Molina & Nerad 1991) Cavalier-Smith 2010 [Amastigomonas bermudensisMolina & Nerad 1991]
• Thecamonadinae Larsen & Patterson 1990 [Thecamonas/Chelomonas clade]
  • Chelonemonas Heiss, Lee, Ishida & Simpson, 2015
    • C. dolaniTorruella, Galindo et al. 2022
    • C. geobukHeiss, Lee, Ishida & Simpson, 2015
    • C. masanensisHeiss, Lee, Ishida & Simpson, 2015
  • Karpovia Torruella, Galindo et al. 2022
    • K. croaticaTorruella, Galindo et al. 2022
  • Singekia Torruella, Galindo et al. 2022
    • S. franciliensisTorruella, Galindo et al. 2022
    • S. montserratensisTorruella, Galindo et al. 2022
  • Thecamonas Larsen & Patterson 1990
    • T. filosaLarsen & Patterson 1990 [Amastigomonas filosa(Larsen & Patterson 1990) Molina & Nerad 1991]
    • T. muscula(Mylnikov 1999) Cavalier-Smith 2010 [Amastigomonas musculaMylnikov 1999]
    • T. mutabilis(Griessmann 1913) Larsen & Patterson 1990 [Rhynchomonas mutabilisGriessmann 1913; Amastigomonas mutabilis(Griessmann 1913) Patterson & Zölffel 1993]
    • T. trahensLarsen & Patterson 1990 [Amastigomonas trahens(Larsen & Patterson 1990) Molina & Nerad 1991]

References

1. Cavalier-Smith, Thomas (May 2013). "Early evolution of eukaryote feeding modes, cell structural diversity, and classification of the protozoan phyla Loukozoa, Sulcozoa, and Choanozoa". European Journal of Protistology. 49 (2): 115–178 Document online. doi:10.1016/j.ejop.2012.06.001. ISSN   0932-4739. PMID   23085100.
2. Karpov SA, Mylnikov AP (1989). "БИОЛОГИЯ И УЛЬТРАСТРУКТУРА БЕСЦВЕТНЫХ ЖГУТИКОНОСЦЕВ APUSOMONADIDA ORD.N" [Biology and ultrastructure of colourless flagellates Apusomonadida ord. n.](PDF). Zoologischkeiĭ Zhurnal (in Russian). LXVIII (8): 5–17.
3. Cavalier-Smith, Thomas; Chao, Ema E. (October 2010). "Phylogeny and evolution of Apusomonadida (Protozoa: Apusozoa): new genera and species". Protist . 161 (4): 549–576. doi:10.1016/j.protis.2010.04.002. PMID   20537943.
4. Brown, Matthew W.; Sharpe, Susan C.; Silberman, Jeffrey D.; Heiss, Aaron A.; Lang, B. Franz; Simpson, Alastair G. B.; Roger, Andrew J. (2013-10-22). "Phylogenomics demonstrates that breviate flagellates are related to opisthokonts and apusomonads". Proceedings of the Royal Society of London B: Biological Sciences. 280 (1769): 20131755. doi:10.1098/rspb.2013.1755. ISSN   0962-8452. PMC   3768317 . PMID   23986111.
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6. Heiss AA, Lee WJ, Ishida KI, Simpson AG (2015). "Cultivation and Characterisation of New Species of Apusomonads (the Sister Group to Opisthokonts), Including Close Relatives of Thecamonas (Chelonemonas n. gen.)". Journal of Eukaryotic Microbiology. 62 (5): 637–649. doi:10.1111/jeu.12220. PMID   25912654.
7. Torruella G, Galindo LJ, Moreira D, Ciobanu M, Heiss AA, Yubuki N, et al. (November 2022). "Expanding the molecular and morphological diversity of Apusomonadida, a deep-branching group of gliding bacterivorous protists". Journal of Eukaryotic Microbiology. 70 (2): e12956. doi:10.1111/jeu.12956. hdl: 2117/404026 .
8. Sebe-Pedros A, Roger AJ, Lang FB, King N, Ruiz-Trillo I (2010). "Ancient origin of the integrin-mediated adhesion and signaling machinery". Proceedings of the National Academy of Sciences of the United States of America. 107 (22): 10142–10147. doi:10.1073/pnas.1002257107. PMC   2890464 .
9. Sebé-Pedrós A, Ruiz-Trillo I (2010). "Integrin-mediated adhesion complex". Communicative & Integrative Biology. 3 (5): 475–477. doi:10.4161/cib.3.5.12603. PMC   2974085 .
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11. Yabuki A, Tame A, Mizuno K (2022). "Podomonas kaiyoae n. sp., a novel apusomonad growing axenically". Journal of Eukaryotic Microbiology. 00: e12946. doi: 10.1111/jeu.12946 .