Ammonia transporter

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Ammonia transporter
Rhcg 3hd6.png
Human Rhesus C Glycoprotein. PDB 3hd6 [1]
Identifiers
SymbolAmtB
Pfam PF00909
InterPro IPR001905
TCDB 1.A.11
OPM superfamily 13
OPM protein 2ns1
Available protein structures:
Pfam   structures / ECOD  
PDB RCSB PDB; PDBe; PDBj
PDBsum structure summary

Ammonia transporters (TC# 1.A.11) are structurally related membrane transport proteins called Amt proteins (ammonia transporters) in bacteria and plants, methylammonium/ammonium permeases (MEPs) in yeast, or Rhesus (Rh) proteins in chordates. In humans, the RhAG, RhBG, and RhCG Rhesus proteins constitute solute carrier family 42 [2] whilst RhD and RhCE form the Rh blood group system. The three-dimensional structure of the ammonia transport protein AmtB from Escherichia coli has been determined by x-ray crystallography [3] [4] revealing a hydrophobic ammonia channel. [5] The human RhCG ammonia transporter was found to have a similar ammonia-conducting channel structure. [1] It was proposed[ citation needed ] that the erythrocyte Rh complex is a heterotrimer of RhAG, RhD, and RhCE subunits in which RhD and RhCE might play roles in anchoring the ammonia-conducting RhAG subunit to the cytoskeleton. Based on reconstitution experiments, purified RhCG subunits alone can function to transport ammonia. [6] RhCG is required for normal acid excretion by the mouse kidney [7] and epididymis. [8]

Contents

Structure

The structure of the ammonia channel from E. coli, [3] [4] was, at the time of its publication, the highest resolution structure of any integral membrane protein. It shows a trimer of subunits, each made up of 11 transmembrane segments (TMSs) and containing a pseudo two-fold symmetry. [9] Each monomer contains a hydrophobic ammonia conducting channel.

While prokaryotic ammonia channel proteins have an N-terminal region which acts as a signal sequence and is cleaved in the mature protein, [10] the Rhesus glycoproteins retain this as a 12th transmembrane helix in the mature protein. [1]

Substrate specificity

Most functionally characterized members of the family are ammonium uptake transporters. [11] Some, but not other Amt proteins also transport methylammonium. [12] [13] Detailed phylogenetic analyses of plant homologues have been published. [14] In E. coli, NH4+, rather than NH3, may be the substrate of AmtB, but controversy still exists. [15] [16] [17] If NH4+ is transported, K+ possibly serves as a counter ion in an antiport process with K+, and that one histidine removes a proton off of NH4+ to yield NH3. [15]

Transport reaction

The generalized transport reaction catalyzed by members of the Amt family are suggested to be:

NH4+ (out) ⇌ NH4+ (in)

Mechanism

The X-ray structures have revealed that the pore of the Amt and Rh proteins is characterized by a hydrophobic portion about 12 Å long, in which electronic density was observed in the crystallographic study of AmtB from Escherichia coli. This electronic density was initially only observed when crystals were grown in the presence of ammonium, and was thus attributed to ammonia molecules. The Amt/Rh protein mechanism might involve the single-file diffusion of NH3 molecules. However, the pore could also be filled with water molecules. The possible presence of water molecules in the pore lumen calls for a reassessment of the notion that Amt/Rh proteins work as plain NH3 channels. Indeed, functional experiments on plant ammonium transporters and Rh proteins suggest a variety of permeation mechanisms including the passive diffusion of NH3, the antiport of NH4+/H+, the transport of NH4+, or the cotransport of NH3/H+. Lamoureux et al. discuss these mechanisms in light of functional and simulation studies on the AmtB transporter. [18]

Regulation

In E. coli the AmtB gene is expressed only under limiting nitrogen levels to yield the AmtB protein. It is co-expressed with the GlnK gene which encodes a PII protein. This protein is also trimeric and remains in the cytoplasm. [19] It is covalently modified by a U/U deuridylylated group at Y51. The hydrolyzed product, adenosine 5'-diphosphate, orients the surface of GlnK for AmtB blockade. When nitrogen levels outside the cell rise, the ammonia channel must be deactivated to prevent excessive ammonia entering the cell (where ammonia would be combined with glutamate to make glutamine, utilizing ATP and thereby depleting the cell's ATP reserves). This deactivation is achieved by deuridylylation of the GlnK protein which then binds to the cytoplasmic face of AmtB and inserts a loop into the ammonia conducting pore. At the tip of this loop is an arginine residue which sterically blocks the channel. [20]

RHAG, RHBG, RHCE, RHCG, RHD

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<i>Nitrosomonas</i> Genus of bacteria

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References

  1. 1 2 3 Gruswitz, F.; Chaudhary, S.; Ho, J. D.; Schlessinger, A.; Pezeshki, B.; Ho, C. -M.; Sali, A.; Westhoff, C. M.; Stroud, R. M. (2010). "Function of human Rh based on structure of RhCG at 2.1 A". Proceedings of the National Academy of Sciences. 107 (21): 9638–9643. doi: 10.1073/pnas.1003587107 . PMC   2906887 . PMID   20457942.
  2. ↑ Nakhoul NL, Hamm LL (Feb 2004). "Non-erythroid Rh glycoproteins: a putative new family of mammalian ammonium transporters". Pflügers Archiv. 447 (5): 807–12. doi:10.1007/s00424-003-1142-8. PMID   12920597. S2CID   24601165.
  3. 1 2 1xqe ; Khademi S, O'Connell J, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM (Sep 2004). "Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A". Science. 305 (5690): 1587–94. CiteSeerX   10.1.1.133.6480 . doi:10.1126/science.1101952. PMID   15361618. S2CID   11436509.
  4. 1 2 2u7c ; Zheng L, Kostrewa D, Bernèche S, Winkler FK, Li XD (Dec 2004). "The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli". Proceedings of the National Academy of Sciences of the United States of America. 101 (49): 17090–5. Bibcode:2004PNAS..10117090Z. doi: 10.1073/pnas.0406475101 . PMC   535379 . PMID   15563598.
  5. ↑ Khademi S, Stroud RM (Dec 2006). "The Amt/MEP/Rh family: structure of AmtB and the mechanism of ammonia gas conduction". Physiology. 21 (6): 419–29. doi:10.1152/physiol.00051.2005. PMID   17119155.
  6. ↑ Mouro-Chanteloup I, Cochet S, Chami M, Genetet S, Zidi-Yahiaoui N, Engel A, Colin Y, Bertrand O, Ripoche P (2010). Fatouros D (ed.). "Functional reconstitution into liposomes of purified human RhCG ammonia channel". PLOS ONE. 5 (1): e8921. Bibcode:2010PLoSO...5.8921M. doi: 10.1371/journal.pone.0008921 . PMC   2812482 . PMID   20126667.
  7. ↑ Wagner CA, Devuyst O, Belge H, Bourgeois S, Houillier P (Jan 2011). "The rhesus protein RhCG: a new perspective in ammonium transport and distal urinary acidification" (PDF). Kidney International. 79 (2): 154–61. doi:10.1038/ki.2010.386. PMID   20927037.
  8. ↑ Biver S, Belge H, Bourgeois S, Van Vooren P, Nowik M, Scohy S, Houillier P, Szpirer J, Szpirer C, Wagner CA, Devuyst O, Marini AM (Nov 2008). "A role for Rhesus factor Rhcg in renal ammonium excretion and male fertility". Nature. 456 (7220): 339–43. Bibcode:2008Natur.456..339B. doi:10.1038/nature07518. PMID   19020613. S2CID   205215412.
  9. ↑ Conroy MJ, Jamieson SJ, Blakey D, Kaufmann T, Engel A, Fotiadis D, Merrick M, Bullough PA (Dec 2004). "Electron and atomic force microscopy of the trimeric ammonium transporter AmtB". EMBO Reports. 5 (12): 1153–8. doi:10.1038/sj.embor.7400296. PMC   1299191 . PMID   15568015.
  10. ↑ Thornton J, Blakey D, Scanlon E, Merrick M (May 2006). "The ammonia channel protein AmtB from Escherichia coli is a polytopic membrane protein with a cleavable signal peptide". FEMS Microbiology Letters. 258 (1): 114–20. doi: 10.1111/j.1574-6968.2006.00202.x . PMID   16630265.
  11. ↑ Soupene, Eric; King, Natalie; Feild, Eithne; Liu, Phillip; Niyogi, Krishna K.; Huang, Cheng-Han; Kustu, Sydney (2002-05-28). "Rhesus expression in a green alga is regulated by CO(2)". Proceedings of the National Academy of Sciences of the United States of America. 99 (11): 7769–7773. Bibcode:2002PNAS...99.7769S. doi: 10.1073/pnas.112225599 . ISSN   0027-8424. PMC   124347 . PMID   12032358.
  12. ↑ Musa-Aziz, Raif; Chen, Li-Ming; Pelletier, Marc F.; Boron, Walter F. (2009-03-31). "Relative CO2/NH3 selectivities of AQP1, AQP4, AQP5, AmtB, and RhAG". Proceedings of the National Academy of Sciences of the United States of America. 106 (13): 5406–5411. Bibcode:2009PNAS..106.5406M. doi: 10.1073/pnas.0813231106 . ISSN   1091-6490. PMC   2664022 . PMID   19273840.
  13. ↑ Andrade, Susana L. A.; Einsle, Oliver (2007-12-01). "The Amt/Mep/Rh family of ammonium transport proteins". Molecular Membrane Biology. 24 (5–6): 357–365. doi: 10.1080/09687680701388423 . ISSN   0968-7688. PMID   17710640. S2CID   41937253.
  14. ↑ von Wittgenstein, Neil J. J. B.; Le, Cuong H.; Hawkins, Barbara J.; Ehlting, Jürgen (2014-01-01). "Evolutionary classification of ammonium, nitrate, and peptide transporters in land plants". BMC Evolutionary Biology. 14: 11. doi: 10.1186/1471-2148-14-11 . ISSN   1471-2148. PMC   3922906 . PMID   24438197.
  15. 1 2 Fong, Rebecca N.; Kim, Kwang-Seo; Yoshihara, Corinne; Inwood, William B.; Kustu, Sydney (2007-11-20). "The W148L substitution in the Escherichia coli ammonium channel AmtB increases flux and indicates that the substrate is an ion". Proceedings of the National Academy of Sciences of the United States of America. 104 (47): 18706–18711. Bibcode:2007PNAS..10418706F. doi: 10.1073/pnas.0709267104 . ISSN   1091-6490. PMC   2141841 . PMID   17998534.
  16. ↑ Ishikita, Hiroshi; Knapp, Ernst-Walter (2007-02-07). "Protonation states of ammonia/ammonium in the hydrophobic pore of ammonia transporter protein AmtB". Journal of the American Chemical Society. 129 (5): 1210–1215. doi:10.1021/ja066208n. ISSN   0002-7863. PMID   17263403.
  17. ↑ Javelle, Arnaud; Lupo, Domenico; Zheng, Lei; Li, Xiao-Dan; Winkler, Fritz K.; Merrick, Mike (2006-12-22). "An unusual twin-his arrangement in the pore of ammonia channels is essential for substrate conductance". The Journal of Biological Chemistry. 281 (51): 39492–39498. doi: 10.1074/jbc.M608325200 . ISSN   0021-9258. PMID   17040913.
  18. ↑ Lamoureux, G.; Javelle, A.; Baday, S.; Wang, S.; Bernèche, S. (2010-09-01). "Transport mechanisms in the ammonium transporter family". Transfusion Clinique et Biologique. 17 (3): 168–175. doi:10.1016/j.tracli.2010.06.004. ISSN   1953-8022. PMID   20674437.
  19. ↑ Durand A, Merrick M (Oct 2006). "In vitro analysis of the Escherichia coli AmtB-GlnK complex reveals a stoichiometric interaction and sensitivity to ATP and 2-oxoglutarate". The Journal of Biological Chemistry. 281 (40): 29558–67. doi: 10.1074/jbc.M602477200 . PMID   16864585.
  20. ↑ 2nuu ; Conroy MJ, Durand A, Lupo D, Li XD, Bullough PA, Winkler FK, Merrick M (Jan 2007). "The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel". Proceedings of the National Academy of Sciences of the United States of America. 104 (4): 1213–8. Bibcode:2007PNAS..104.1213C. doi: 10.1073/pnas.0610348104 . PMC   1783118 . PMID   17220269.

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