Bioremediation of radioactive waste

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Bioremediation of radioactive waste or bioremediation of radionuclides is an application of bioremediation based on the use of biological agents bacteria, plants and fungi (natural or genetically modified) to catalyze chemical reactions that allow the decontamination of sites affected by radionuclides. [1] These radioactive particles are by-products generated as a result of activities related to nuclear energy and constitute a pollution and a radiotoxicity problem (with serious health and ecological consequences) due to its unstable nature of ionizing radiation emissions.

Contents

The techniques of bioremediation of environmental areas as soil, water and sediments contaminated by radionuclides are diverse and currently being set up as an ecological and economic alternative to traditional procedures. Physico-chemical conventional strategies are based on the extraction of waste by excavating and drilling, with a subsequent long-range transport for their final confinement. These works and transport have often unacceptable estimated costs of operation that could exceed a trillion dollars in the US and 50 million pounds in the UK. [2]

The species involved in these processes have the ability to influence the properties of radionuclides such as solubility, bioavailability and mobility to accelerate its stabilization. Its action is largely influenced by electron donors and acceptors, nutrient medium, complexation of radioactive particles with the material and environmental factors. These are measures that can be performed on the source of contamination ( in situ ) or in controlled and limited facilities in order to follow the biological process more accurately and combine it with other systems ( ex situ ). [3] [4]

Areas contaminated by radioactivity

Typology of radionuclides and polluting waste

The presence of radioactive waste in the environment may cause long-term effects due to the activity and half-life of the radionuclides, leading their impact to grow with time. [2] These particles exist in various oxidation states and are found as oxides, coprecipitates, or as organic or inorganic complexes, according to their origin and ways of liberation. Most commonly they are found in oxidized form, which makes them more soluble in water and thus more mobile. [4] Unlike organic contaminants, however, they cannot be destroyed and must be converted into a stable form or extracted from the environment. [5]

The sources of radioactivity are not exclusive of human activity. Natural radioactivity does not come from human sources: it covers up to three fourths of the total radioactivity in the world and has its origins in the interaction of terrestrial elements with high energy cosmic rays (cosmogenic radionuclides) or in the existing materials on Earth since its formation (primordial radionuclides). In this regard, there are differences in the levels of radioactivity throughout the Earth's crust. India and mountains like the Alps are among the areas with the highest level of natural radioactivity due to their composition of rocks and sand. [6]

The most frequent radionuclides in soils are naturally radium-226 (226Ra), radon-222 (222Rn), thorium-232 (232Th), uranium-238 (238U) and potassium-40 (40K). Potassium-40 (up to 88% of total activity), carbon-14 (14C), radium-226, uranium-238 and rubidium-87 (87Rb) are found in ocean waters. Moreover, in groundwater abound radius radioisotopes such as radium-226 and radium-228 (228Ra). [7] [8] They are also habitual in building materials radionuclides of uranium, thorium and potassium (the latter common to wood). [8]

At the same time, anthropogenic radionuclides (caused by humans) are due to thermonuclear reactions resulting from explosions and nuclear weapons tests, discharges from nuclear facilities, accidents deriving from the reprocessing of commercial fuel, waste storage from these processes and to a lesser extent, nuclear medicine. [9] Some polluted sites by these radionuclides are the US DOE facilities (like Hanford Site), the Chernobyl and Fukushima exclusion zones and the affected area of Chelyabinsk Oblast due to the Kyshtym disaster.

In ocean waters, the presence of tritium (3H), cesium-137 (137Cs), strontium-90 (90Sr), plutonium-239 (239Pu) and plutonium-240 (240Pu) has significantly increased due to anthropogenic causes. [10] [11] In soils, technetium-99 (99Tc), carbon-14, strontium-90, cobalt-60 (60Co), iodine-129 (129I), iodine-131 (131I), americium-241 (241Am), neptunium-237 (237Np) and various forms of radioactive plutonium and uranium are the most common radionuclides. [2] [8] [9]

Frequency of occurrence of selected radionuclides at US DOE facilities
Ground waterSoils/Sediments
Bioremediation of radioactive waste
Bioremediation of radioactive waste
Source: United States Department of Energy, US Government (1992) [12]

The classification of radioactive waste established by the International Atomic Energy Agency (IAEA) distinguishes six levels according to equivalent dose, specific activity, heat released and half-life of the radionuclides: [13]

Ecological and human health consequences

Radioactive contamination is a potential danger for living organisms and results in external hazards, concerning radiation sources outside the body, and internal dangers, as a result of the incorporation of radionuclides inside the body (often by inhalation of particles or ingestion of contaminated food). [14]

In humans, single doses from 0.25 Sv produce first anomalies in the amount of leukocytes. This effect is accentuated if the absorbed dose is between 0.5 and 2 Sv, in whose first damage, nausea and hair loss are suffered. The strip ranging between 2 and 5 Sv is considered the most serious and include bleeding, ulcers and risk of death; values exceeding 5 Sv involve immediate death. [14] If radiation, likewise, is received in small doses over long periods of time, the consequences can be equally severe. It is difficult to quantify the health effects for doses below 10 mSv, but it has been shown that there is a direct relationship between prolonged exposure and cancer risk (although there is not a very clear dose-response relationship to establish clear limits of exposure). [15]

The information available on the effect of natural background radiation with respect anthropogenic pollution on wildlife is scarce and refers to very few species. It is very difficult to estimate from the available data the total doses that can accumulate during specific stages of the life cycle (embryonic development or reproductive age), in changes in behavior or depending on environmental factors such as seasonality. [16] The phenomena of radioactive bioaccumulation, bioconcentration and biomagnification, however, are especially known to sea level. They are caused by the recruitment and retention of radioisotopes by bivalves, crustaceans, corals and phytoplankton, which then amounted to the rest of the food chain at low concentration factors. [17]

Radiobiological literature and IAEA establish a safe limit of absorbed dose of 0.001 Gy/d for terrestrial animals and 0.01 Gy/d for plants and marine biota, although this limit should be reconsidered for long-lived species with low reproductive capacity. [18]

1909 study in which the effect of exposure to radioactive radium on lupins is shown. The radiological activity was the same for all seedlings, but not the duration of exposure (descending from left to right, the fourth as control). Those exposed for longer suffered more damage and higher growth and germination deficiences. PSM V74 D230 Three plants irradiated with a fourth as the control.png
1909 study in which the effect of exposure to radioactive radium on lupins is shown. The radiological activity was the same for all seedlings, but not the duration of exposure (descending from left to right, the fourth as control). Those exposed for longer suffered more damage and higher growth and germination deficiences.

Radiation tests in model organisms that determine the effects of high radiation on animals and plants are: [18]

The effects of radioactivity on bacteria are given, as in eukaryotes, by ionization of water and production of reactive oxygen species. These compounds mutate DNA strands and produce genetic damage, inducing newly lysis and subsequent cell death. [20] [21]

Its action on viruses, on the other hand, results in damaged nucleic acids and viral inactivation. [22] They have a sensory threshold ranging between 1000 and 10,000 Gy (range occupying most biological organisms) which decreases with increasing genome size. [23]

Bacterial bioremediation

The biochemical transformation of radionuclides into stable isotopes by bacterial species significantly differs from the metabolism of organic compounds coming from carbon sources. They are highly energetic radioactive forms which can be converted indirectly by the process of microbial energy transfer. [1]

Radioisotopes can be transformed directly through changes in valence state by acting as acceptors or by acting as cofactors to enzymes. They can also be transformed indirectly by reducing and oxidizing agents produced by microorganisms that cause changes in pH or redox potential. Other processes include precipitation and complexation of surfactants, or chelating agents that bind to radioactive elements. Human intervention, on the other hand, can improve these processes through genetic engineering and omics, or by injection of microorganisms or nutrients into the treatment area. [1] [5]

Bioreduction

According to the radioactive element and the specific site conditions, bacteria can enzymatically immobilize radionuclides directly or indirectly. Their redox potential is exploited by some microbial species to carry out reductions that alter the solubility and hence, mobility, bioavailability and radiotoxicity. This waste treatment technique called bioreduction or enzymatic biotransformation is very attractive because it can be done in mild conditions for the environment, does not produce hazardous secondary waste and has potential as a solution for waste of various kinds. [4]

Depiction of direct enzymatic reduction. Microorganisms use organic compounds as lactate, acetate or formate as electron donors to reduce and leave radionuclides in insoluble form. Enzymatic reduction of radionuclides (en).png
Depiction of direct enzymatic reduction. Microorganisms use organic compounds as lactate, acetate or formate as electron donors to reduce and leave radionuclides in insoluble form.

Direct enzymatic reduction is the change of radionuclides of a higher oxidation state to a lower one made by facultative and obligate anaerobes. The radioisotope interact with binding sites of metabolically active cells and is used as terminal electron acceptor in the electron transport chain where compounds such as ethyl lactate act as electron donors under anaerobic respiration. [4]

The periplasm plays a very important role in these bioreductions. In the reduction of uranium (VI) to insoluble uranium (IV), made by Shewanella putrefaciens , Desulfovibrio vulgaris , Desulfovibrio desulfuricans and Geobacter sulfurreducens , the activity of periplasmic cytochromes is required. The reduction of technetium (VII) to technetium (IV) made by S. putrefaciens, G. sulfurreducens, D. desulfuricans, Geobacter metallireducens and Escherichia coli , on the other hand, requires the presence of the complex formate hydrogenlyase, also placed in this cell compartment. [2]

Other radioactive actinides such as thorium, plutonium, neptunium and americium are enzymatically reduced by Rhodoferax ferrireducens , S. putrefaciens and several species of Geobacter , and directly form an insoluble mineral phase. [2]

The phenomenon of indirect enzymatic reduction is carried out by sulfate-reducing and dissimilatory metal-reducing bacteria on excretion reactions of metabolites and breakdown products. There is a coupling of the oxidation of organic acids —produced by the excretion of these heterotrophic bacteria— with the reduction of iron or other metals and radionuclides, which forms insoluble compounds that can precipitate as oxide and hydroxide minerals. In the case of sulfate-reducing bacteria hydrogen sulfide is produced, promoting increased solubility of polluting radionuclides and their bioleaching (as liquid waste that can then be recovered). [2] [4]

There are several species of reducing microorganisms that produce indirect sequestering agents and specific chelators, such as siderophores. These sequestering agents are crucial in the complexation of radionuclides and increasing their solubility and bioavailability. Microbacterium flavescens , for example, grows in the presence of radioisotopes such as plutonium, thorium, uranium or americium and produces organic acids and siderophores that allow the dissolution and mobilization of radionuclides through the soil. It seems that siderophores on bacterial surface could also facilitate the entry of these elements within the cell as well. Pseudomonas aeruginosa also secretes chelating agents out that meet uranium and thorium when grown in a medium with these elements. In general, it has also been found that enterobactin siderophores are extremely effective in solubilizing actinide oxides of plutonium. [2] [4]

Citrate complexes

Citrate is a chelator which binds to certain transition metals and radioactive actinides. Stable complexes such as bidentate, tridentate (ligands with more than one atom bound) and polynuclear complexes (with several radioactive atoms) can be formed with citrate and radionuclides, which receive a microbial action. Anaerobically, Desulfovibrio desulfuricans and species of the genera Shewanella and Clostridium are able to reduce bidentate complexes of uranyl-citrate (VI) to uranyl-citrate (IV) and make them precipitate, despite not being able to degrade metabolically complexed citrate at the end of the process. [2] In denitrifying and aerobic conditions, however, it has been determined that it is not possible to reduce or degrade these uranium complexes. Bioreduction do not get a head when they are citrate complex mixed metal complexes or when they are tridentate, monomeric or polynuclear complexes, since they become recalcitrant and persistent in the environment. [4] [24] From this knowledge exists a system that combines the degradation of radionuclide-citrate complex with subsequent photodegradation of remaining reduced uranyl-citrate (previously not biodegradated but sensitive to light), which allows for stable precipitates of uranium and also of thorium, strontium or cobalt from contaminated lands. [4]

Biosorption, bioaccumulation and biomineralization

Biosorption, bioaccumulation and biomineralization strategies with a specific role for each cell compartment. Biosorption, biomineralization and bioaccumulation of radionuclides (en).png
Biosorption, bioaccumulation and biomineralization strategies with a specific role for each cell compartment.

The set of strategies that comprise biosorption, bioaccumulation and biomineralization are closely related to each other, because one way or another have a direct contact between the cell and radionuclide. These mechanisms are evaluated accurately using advanced analysis technologies such as electron microscopy, X-ray diffraction and XANES, EXAFS and X-ray spectroscopies. [1] [25]

Biosorption and bioaccumulation are two metabolic actions that are based on the ability to concentrate radionuclides over a thousand times the concentration of the environment. They consist of complexation of radioactive waste with phosphates, organic compounds and sulfites so that they become insoluble and less exposed to radiotoxicity. They are particularly useful in biosolids for agricultural purposes and soil amendments, although most properties of these biosolids are unknown. [26]

Biosorption method is based on passive sequestration of positively charged radioisotopes by lipopolysaccharides (LPS) on the cell membrane (negatively charged), either live or dead bacteria. Its efficiency is directly related to the increase in temperature and can last for hours, being a much faster method than direct bioreduction. It occurs through the formation of slimes and capsules, and with a preference for binding to the phosphate and phosphoryl groups (although it also occurs with carboxyl, amine or sulfhydryl groups). Bacillota and other bacteria like Citrobacter freudii have significant biosorption capabilities; Citrobacter does it through electrostatic interaction of uranium with phosphates of their LPS. [2] [3]

Quantitative analyzes determine that, in the case of uranium, biosorption may vary within a range between 45 and 615 milligrams per gram of cell dry weight. However, it is a technique that requires a high amount of biomass to affect bioremediation; it presents problems of saturation and other cations that compete for binding to the bacterial surface. [3]

Bioaccumulation refers to uptake of radionuclides into the cell, where they are retained by complexations with negatively charged intracellular components, precipitation or granules formations. Unlike biosorption, this is an active process: it depends on an energy-dependent transport system.[ citation needed ] Some metals or radionuclides can be absorbed by bacteria accidentally because of its resemblance to dietary elements for metabolic pathways. Several radioisotopes of strontium, for example, are recognized as analogs of calcium and incorporated within Micrococcus luteus . [4] Uranium, however, has no known function and is believed that its entry into the cell interior may be due to its toxicity (it is able to increase membrane permeability). [3]

Chernikovite and meta-autunite, radioactive minerals result of possible biomineralization. Chernikovite 1.jpg
Chernikovite and meta-autunite, radioactive minerals result of possible biomineralization.

Furthermore, biomineralization —also known as bioprecipitation— is the precipitation of radionuclides through the generation of microbial ligands, resulting in the formation of stable biogenic minerals. These minerals have a very important role in the retention of radioactive contaminants. A very localized and produced enzymatically ligand concentration is involved and provides a nucleation site for the onset of biomineral precipitation. [27] This is particularly relevant in precipitations of phosphatase activity-derivate biominerals, which cleavage molecules such as glycerol phosphate on periplasm. In Citrobacter and Serratia genera, this cleavage liberates inorganic phosphates (HPO42−) that precipitates with uranyl ion (UO22+) and cause deposition of polycrystalline minerals around the cell wall. [2] [28] Serratia also form biofilms that promote precipitation of chernikovite (rich in uranium) and additionally, remove up to 85% of cobalt-60 and 97% of cesium-137 by proton substitution of this mineral. [25] In general, biomineralization is a process in which the cells do not have limitations of saturation and can accumulate up to several times its own weight as precipitated radionuclides. [4]

Investigations of terrestrial and marine bacterial isolates belonging to the genera Aeromonas , Bacillus , Myxococcus , Pantoea , Pseudomonas , Rahnella and Vibrio have also demonstrated the removal of uranium radioisotopes as phosphate biominerals in both oxic and anoxic growth conditions. [25]

Biostimulation and bioaugmentation

Evolution of the Old Rifle UMTRA Site (Colorado, US) from 1957 (above) until 2008 (below), in which biostimulation tasks were carried out. Evolution of the Old Rifle Processing Site.jpeg
Evolution of the Old Rifle UMTRA Site (Colorado, US) from 1957 (above) until 2008 (below), in which biostimulation tasks were carried out.

Aside from bioreduction, biosorption, bioaccumulation and biomineralization, which are bacterial strategies for natural attenuation of radioactive contamination, there are also human methods that increase the efficiency or speed of microbial processes. This accelerated natural attenuation involves an intervention in the contaminated area to improve conversion rates of radioactive waste, which tend to be slow. There are two variants: biostimulation and bioaugmentation. [30]

Biostimulation is the addition of nutrients with trace elements, electron donors or electron acceptors to stimulate activity and growth of natural indigenous microbial communities. [4] [30] It can range from simple fertilization or infiltration (called passive biostimulation) to more aggressive injections to the ground, and is widely used at US DOE sites. [26] Nitrate is used as nutrient to biostimulate the reduction of uranium, because it serves as very energetically favorable electron acceptor for metal-reducing bacteria. However, many of these microorganisms ( Geobacter , Shewanella or Desulfovibrio ) exhibit resistance genes to heavy metals that limit their ability to bioremediate radionuclides. In these particular cases, a carbon source such as ethanol is added to the medium to promote the reduction of nitrate at first, and then of uranium. Ethanol is also used in soil injection systems with hydraulic recirculations: it raises the pH and promotes the growth of denitrifying and radionuclide-reducing bacteria, that produce biofilms and achieve almost 90% decrease in the concentration of radioactive uranium. [2]

A number of geophysical techniques have been used to monitor the effects of in situ biostimulation trials including measurement of: spectral ionization potential, self potentials, current density, complex resistivity and also reactive transport modelling (RTM), which measures hydrogeological and geochemical parameters to estimate chemical reactions of the microbial community. [3]

Bioaugmentaton, on the other hand, is the deliberated addition to the environment of microorganisms with desired traits to accelerate bacterial metabolic conversion of radioactive waste. They are often added when necessary species for bioremediation do not exist in the treatment place. [4] [30] This technique has shown in field trials over the years that it does not offer better results than biostimulation; neither it is clear that introduced species can be distributed effectively through the complex geological structures of most subsurface environments or that can compete long term with the indigenous microbiota. [1] [26]

Genetic engineering and omics

Deinococcus radiodurans has much interest in genetic engineering for bioremediation of radioactive waste. Deinococcus radiodurans.jpg
Deinococcus radiodurans has much interest in genetic engineering for bioremediation of radioactive waste.

Omics, especially genomics and proteomics, allow identifying and evaluating genes, proteins and enzymes involved in radionuclide bioremediation, apart from the structural and functional interactions that exist between them and other metabolites. Genome sequencing of various microorganisms has uncovered, for example, that Geobacter sulfurreducens possess more than 100 coding regions for c-type cytochromes involved in bioremediation radionuclide, or that NiCoT gene is significantly overexpressed in Rhodopseudomonas palustris and Novosphingobium aromaticivorans when grown in medium with radioactive cobalt. [1] [2]

From this information, different genetic engineering and recombinant DNA techniques are being developed to generate specific bacteria for bioremediation. Some constructs expressed in microbial species are phytochelatins, polyhistidines and other polypeptides by fusion-binding domains to outer-membrane-anchored proteins. [2] Some of these genetically modified strains are derived from Deinococcus radiodurans , one of the most radiation-resistant organisms. D. radiodurans is capable to resist oxidative stress and DNA damage from radiation, and reduces technetium, uranium and chromium naturally as well. Besides, through insertion of genes from other species it has been achieved that it can also precipitates uranyl phosphates and degrades mercury by using toluene as an energy source to grow and stabilize other priority radionuclides. [1] [3]

Directed evolution of bacterial proteins related to bioremediation of radionuclides is also a field research. YieF enzyme, for example, naturally catalyzes the reduction of chromium with a very wide range of substrates. Following protein engineering, however, it has also been able to participate in uranyl ion reduction. [31]

Plant bioremediation

Phytoremediation processes. Radionuclides can not be phytodegraded but converted to more stable or less toxic forms. Phytoremediation Process.svg
Phytoremediation processes. Radionuclides can not be phytodegraded but converted to more stable or less toxic forms.

The use of plants to remove contaminants from the environment or to render them less harmful is called phytoremediation. In the case of radionuclides, it is a viable technology when decontamination times are long and waste are scattered at low concentrations. [32] [33]

Some plant species are able to transform the state of radioisotopes (without suffering toxicity) concentrating them in different parts of their structure, making them rush through the roots, making them volatile or stabilizing them on the ground. As in bacteria, plant genetic engineering procedures and biostimulation —called phytostimulation— have improved and accelerate these processes, particularly with regard to fast-growing plants. [33] The use of Agrobacterium rhizogenes , for example, is quite widespread and significantly increases radionuclide uptake by the roots.[ citation needed ]

Phytoextraction

In phytoextraction (also phytoaccumulation, phytosequesteration or phytoabsorption) [34] plants carry radioactive waste from the root system to the vascular tissue and become concentrated in the biomass of shoots. It is a technique that removes radionuclides without destroying the soil structure, with minimal impact on soil fertility and valid for large areas with a low level of radioactivity. Its efficiency is evaluated through bioaccumulation coefficient (BC) or total removal of radionuclides per m2, and is proven to attract cesium-137, strontium-90, technetium-99, cerium-144, plutonium-240, americium-241, neptunium-237 and various radioisotopes of thorium and radium. [33] By contrast, it requires large biomass production in short periods of time.[ citation needed ]

Species like common heather or amaranths are able to concentrate cesium-137, the most abundant radionuclide in the Chernobyl Exclusion Zone. In this region of Ukraine, mustard greens could remove up to 22% of average levels of cesium activity in a single growing season. In the same way, bok choy and mustard greens can concentrate 100 times more uranium than other species. [33]

Rhizofiltration

Connected pond system at River Dearne (England). Reedbeds - geograph.org.uk - 380204.jpg
Connected pond system at River Dearne (England).

Rhizofiltration is the adsorption and precipitation of radionuclides in plant roots or absorption thereof if soluble in effluents. It has great efficiency in the treatment of cesium-137 and strontium-90, particularly by algae and aquatic plants, such as Cladophora and Elodea genera, respectively. It is the most efficient strategy for bioremediation technologies in wetlands, [34] but must have a continuous and rigorous control of pH to make it an optimal process. [35]

From this process, some strategies have been designed based on sequences of ponds with a slow flow of water to clean polluted water with radionuclides. The results of these facilities, for flows of 1000 liters of effluent are about 95% retention of radiation in the first pond (by plants and sludge), and over 99% in three-base systems. [33]

The most promising plants for rhizofiltration are sunflowers. They are able to remove up to 95% of uranium of contaminated water in 24 hours, and experiments in Chernobyl have demonstrated that they can concentrate on 55 kg of plant dry weight all the cesium and strontium radioactivity from an area of 75 m2 (stabilized material suitable for transfer to a nuclear waste repository). [33]

Phytovolatilization

Phytovolatilization involves the capture and subsequent transpiration of radionuclides into the atmosphere. It does not remove contaminants but releases them in volatile form (less harmful). Despite not having too many applications for radioactive waste, it is very useful for the treatment of tritium, because it exploits plants' ability to transpire enormous amounts of water. [33] [34]

The treatment applied to tritium (shielded by air produces almost no external radiation exposure, but its incorporation in water presents a health hazard when absorbed into the body) uses polluted effluents to irrigate phreatophytes. It becomes a system with a low operation cost and low maintenance, with savings of about 30% in comparison to conventional methods of pumping and covering with asphalt. [33]

Phytostabilization

Phytostabilization is an specially valid strategy for radioactive contamination based on the immobilization of radionuclides in the soil by the action of the roots. This can occur by adsorption, absorption and precipitation within root zone, and ensures that radioactive waste can not be dispersed because soil erosion or leaching. It is useful in controlling tailings from strip and open pit uranium mines, and guarantees to retrieve the ecosystem. [33] [34] However, it has significant drawbacks such as large doses of fertilizer needed to reforest the area, apart from radioactive source (which implies long-term maintenance) remaining at the same place.[ citation needed ]

Fungal bioremediation

Radiotrophic fungi growth has been described in reactor 4 at the Chernobyl Nuclear Power Station. Chernobyl nuclear plant2.jpg
Radiotrophic fungi growth has been described in reactor 4 at the Chernobyl Nuclear Power Station.

Several fungi species have radioactive resistance values equal to or greater than more radioresistant bacteria; they perform mycoremediation processes. It was reported that some fungi had the ability of growing into, feeding, generating spores and decomposing pieces of graphite from destroyed reactor No. 4 at the Chernobyl Nuclear Power Station, which is contaminated with high concentrations of cesium, plutonium and cobalt radionuclides. They were called radiotrophic fungi. [36]

Since then, it has been shown that some species of Penicillium , Cladosporium , Paecilomyces and Xerocomus are able to use ionizing radiation as energy through the electronic properties of melanins. [36] [37] In their feeding they bioaccumulate radioisotopes, creating problems on concrete walls of deep geological repositories. [38] Other fungi like oyster mushrooms can bioremediate plutonium-239 and americium-241. [39]

Ways of research

Current research on bioremediation techniques is fairly advanced and molecular mechanisms that govern them are well known. However, there are many doubts about the effectiveness and possible adversities of these processes in combination with the addition of agrochemicals. In soils, the role of mycorrhizae on radioactive waste is poorly described and sequestration patterns of radionuclides are not known with certainty. [40]

Longevity effects of some bacterial processes, such as maintenance of uranium in insoluble form because of bioreductions or biomineralizations, are unknown. There are not clear details about the electronic transfer from some radionuclides with these bacterial species either. [3]

Another important aspect is the change of ex situ or laboratory scale processes to their real application in situ , in which soil heterogeneity and environmental conditions generate reproduction deficiencies of optimal biochemical status of the used species, a fact that decreases the efficiency. This implies finding what are the best conditions in which to carry out an efficient bioremediation with anions, metals, organic compounds or other chelating radionuclides that can compete with the uptake of interest radioactive waste. [2] Nevertheless, in many cases research is focused on the extraction of soil and water and its ex situ biological treatment to avoid these problems. [4]

Finally, the potential of GMOs is limited by regulatory agencies in terms of responsibility and bioethical issues. Their release require support on the action zone and comparability with indigenous species. Multidisciplinary research is focused on defining more precisely necessary genes and proteins to establish new cell-free systems which may avoid possible side effects on the environment by the intrusion of transgenic or invasive species. [2]

See also

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Biological augmentation is the addition of archaea or bacterial cultures required to speed up the rate of degradation of a contaminant. Organisms that originate from contaminated areas may already be able to break down waste, but perhaps inefficiently and slowly.

<i>Geobacter</i> Genus of anaerobic bacteria found in soil

Geobacter is a genus of bacteria. Geobacter species are anaerobic respiration bacterial species which have capabilities that make them useful in bioremediation. Geobacter was found to be the first organism with the ability to oxidize organic compounds and metals, including iron, radioactive metals, and petroleum compounds into environmentally benign carbon dioxide while using iron oxide or other available metals as electron acceptors. Geobacter species are also found to be able to respire upon a graphite electrode. They have been found in anaerobic conditions in soils and aquatic sediment.

<span class="mw-page-title-main">Radiochemistry</span> Chemistry of radioactive materials

Radiochemistry is the chemistry of radioactive materials, where radioactive isotopes of elements are used to study the properties and chemical reactions of non-radioactive isotopes. Much of radiochemistry deals with the use of radioactivity to study ordinary chemical reactions. This is very different from radiation chemistry where the radiation levels are kept too low to influence the chemistry.

<span class="mw-page-title-main">Mycoremediation</span> Process of using fungi to degrade or sequester contaminants in the environment

Mycoremediation is a form of bioremediation in which fungi-based remediation methods are used to decontaminate the environment. Fungi have been proven to be a cheap, effective and environmentally sound way for removing a wide array of contaminants from damaged environments or wastewater. These contaminants include heavy metals, organic pollutants, textile dyes, leather tanning chemicals and wastewater, petroleum fuels, polycyclic aromatic hydrocarbons, pharmaceuticals and personal care products, pesticides and herbicides in land, fresh water, and marine environments.

<span class="mw-page-title-main">Caesium-137</span> Radioactive isotope of caesium

Caesium-137, cesium-137 (US), or radiocaesium, is a radioactive isotope of caesium that is formed as one of the more common fission products by the nuclear fission of uranium-235 and other fissionable isotopes in nuclear reactors and nuclear weapons. Trace quantities also originate from spontaneous fission of uranium-238. It is among the most problematic of the short-to-medium-lifetime fission products. Caesium-137 has a relatively low boiling point of 671 °C (1,240 °F) and easily becomes volatile when released suddenly at high temperature, as in the case of the Chernobyl nuclear accident and with atomic explosions, and can travel very long distances in the air. After being deposited onto the soil as radioactive fallout, it moves and spreads easily in the environment because of the high water solubility of caesium's most common chemical compounds, which are salts. Caesium-137 was discovered by Glenn T. Seaborg and Margaret Melhase.

<span class="mw-page-title-main">Spent nuclear fuel</span> Nuclear fuel thats been irradiated in a nuclear reactor

Spent nuclear fuel, occasionally called used nuclear fuel, is nuclear fuel that has been irradiated in a nuclear reactor. It is no longer useful in sustaining a nuclear reaction in an ordinary thermal reactor and, depending on its point along the nuclear fuel cycle, it will have different isotopic constituents than when it started.

<span class="mw-page-title-main">Actinides in the environment</span>

The actinide series is a group of chemical elements with atomic numbers ranging from 89 to 102, including notable elements such as uranium and plutonium. The nuclides thorium-232, uranium-235, and uranium-238 occur primordially, while trace quantities of actinium, protactinium, neptunium, and plutonium exist as a result of radioactive decay and neutron capture of uranium. These elements are far more radioactive than the naturally occurring thorium and uranium, and thus have much shorter half-lives. Elements with atomic numbers greater than 94 do not exist naturally on Earth, and must be produced in a nuclear reactor. However, certain isotopes of elements up to californium still have practical applications which take advantage of their radioactive properties.

<span class="mw-page-title-main">Environmental radioactivity</span> Radioactivity naturally present within the Earth

Environmental radioactivity is part of the overall background radiation and is produced by radioactive materials in the human environment. While some radioisotopes, such as strontium-90 (90Sr) and technetium-99 (99Tc), are only found on Earth as a result of human activity, and some, like potassium-40 (40K), are only present due to natural processes, a few isotopes, such as tritium (3H), result from both natural processes and human activities. The concentration and location of some natural isotopes, particularly uranium-238 (238U), can be affected by human activity, such as nuclear weapons testing, which caused a global fallout, with up to 2.4 million deaths by 2020.

<span class="mw-page-title-main">Strontium-90</span> Radioactive isotope of strontium

Strontium-90 is a radioactive isotope of strontium produced by nuclear fission, with a half-life of 28.8 years. It undergoes β− decay into yttrium-90, with a decay energy of 0.546 MeV. Strontium-90 has applications in medicine and industry and is an isotope of concern in fallout from nuclear weapons, nuclear weapons testing, and nuclear accidents.

<span class="mw-page-title-main">Plutonium in the environment</span> Plutonium present within the environment

Since the mid-20th century, plutonium in the environment has been primarily produced by human activity. The first plants to produce plutonium for use in Cold War atomic bombs were the Hanford nuclear site, in Washington, and Mayak nuclear plant, in Chelyabinsk Oblast, Russia. Over a period of four decades, "both released more than 200 million curies of radioactive isotopes into the surrounding environment – twice the amount expelled in the Chernobyl disaster in each instance."

<span class="mw-page-title-main">Environmental impact of nuclear power</span>

Nuclear power has various environmental impacts, both positive and negative, including the construction and operation of the plant, the nuclear fuel cycle, and the effects of nuclear accidents. Nuclear power plants do not burn fossil fuels and so do not directly emit carbon dioxide. The carbon dioxide emitted during mining, enrichment, fabrication and transport of fuel is small when compared with the carbon dioxide emitted by fossil fuels of similar energy yield, however, these plants still produce other environmentally damaging wastes. Nuclear energy and renewable energy have reduced environmental costs by decreasing CO2 emissions resulting from energy consumption.

<span class="mw-page-title-main">Phototrophic biofilm</span> Microbial communities including microorganisms which use light as their energy source

Phototrophic biofilms are microbial communities generally comprising both phototrophic microorganisms, which use light as their energy source, and chemoheterotrophs. Thick laminated multilayered phototrophic biofilms are usually referred to as microbial mats or phototrophic mats. These organisms, which can be prokaryotic or eukaryotic organisms like bacteria, cyanobacteria, fungi, and microalgae, make up diverse microbial communities that are affixed in a mucous matrix, or film. These biofilms occur on contact surfaces in a range of terrestrial and aquatic environments. The formation of biofilms is a complex process and is dependent upon the availability of light as well as the relationships between the microorganisms. Biofilms serve a variety of roles in aquatic, terrestrial, and extreme environments; these roles include functions which are both beneficial and detrimental to the environment. In addition to these natural roles, phototrophic biofilms have also been adapted for applications such as crop production and protection, bioremediation, and wastewater treatment.

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