Aflatoxin B1

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Contents

Aflatoxin B1
(-)-Aflatoxin B1 Structural Formulae V.1.svg
Chemical structure of (−)-aflatoxin B1
Aflatoxin-B1-from-xtal-3D-bs-17.png
Ball-and-stick model of aflatoxin B1 [1] [2]
Names
Preferred IUPAC name
(6aR,9aS)-4-Methoxy-2,3,6a,9a-tetrahydrocyclopenta[c]furo[3′,2′:4,5]furo[2,3-h][1]benzopyran-1,11-dione
Other names
NSC 529592
Identifiers
3D model (JSmol)
ChEMBL
ChemSpider
ECHA InfoCard 100.013.276 OOjs UI icon edit-ltr-progressive.svg
PubChem CID
UNII
  • InChI=1S/C17H12O6/c1-20-10-6-11-14(8-4-5-21-17(8)22-11)15-13(10)7-2-3-9(18)12(7)16(19)23-15/h4-6,8,17H,2-3H2,1H3 Yes check.svgY
    Key: OQIQSTLJSLGHID-UHFFFAOYSA-N Yes check.svgY
  • O=C5C=4C(=O)Oc3c1c(OC2O\C=C/C12)cc(OC)c3C=4CC5
Properties
C17H12O6
Molar mass 312.277 g·mol−1
Hazards
Occupational safety and health (OHS/OSH):
Main hazards
 Carcinogen – Mutagen – Acute toxicity / Poison [3]
GHS labelling:
GHS-pictogram-skull.svg GHS-pictogram-silhouette.svg
Danger
H300, H310, H330, H340, H350
P201, P202, P260, P262, P264, P270, P271, P280, P281, P284, P301+P330+P331, P302+P350, P304+P340, P308+P313, P310, P311, P320, P321, P322, P330, P361, P363, P403+P233, P405, P501
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
X mark.svgN  verify  (what is  Yes check.svgYX mark.svgN ?)

Aflatoxin B1 is an aflatoxin produced by Aspergillus flavus and A. parasiticus . It is a very potent carcinogen with a TD50 3.2 μg/kg/day in rats. [4] This carcinogenic potency varies across species with some, such as rats and monkeys, seemingly much more susceptible than others. [5] [6] Aflatoxin B1 is a common contaminant in a variety of foods including peanuts, cottonseed meal, corn, and other grains; [7] as well as animal feeds. [8] Aflatoxin B1 is considered the most toxic aflatoxin and it is highly implicated in hepatocellular carcinoma (HCC) in humans. [9] In animals, aflatoxin B1 has also been shown to be mutagenic, [10] teratogenic, [11] and to cause immunosuppression. [12] Several sampling and analytical methods including thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), mass spectrometry, and enzyme-linked immunosorbent assay (ELISA), among others, have been used to test for aflatoxin B1 contamination in foods. [13] According to the Food and Agriculture Organization (FAO), a division of the United Nations, the worldwide maximum tolerated levels of aflatoxin B1 was reported to be in the range of 1–20 μg/kg (or .001 ppm - 1 part-per-billion) in food, and 5–50 μg/kg (.005 ppm) in dietary cattle feed in 2003. [14]

Sources of exposure

Aflatoxin B1 is mostly found in contaminated food and humans are exposed to aflatoxin B1 almost entirely through their diet. [15] Occupational exposure to aflatoxin B1 has also been reported in swine [16] and poultry production. [17] While aflatoxin B1 contamination is common in many staple foods, its production is maximized in foods stored in hot, humid climates. [18] Exposure is therefore most common in Southeast Asia, South America, and Sub-Saharan Africa. [18]

Pathology

Aflatoxin B1 can permeate through the skin. Dermal exposure to this aflatoxin in particular environmental conditions can lead to major health risks. [19] The liver is the most susceptible organ to aflatoxin B1 toxicity. In animal studies, pathological lesions associated with aflatoxin B1 intoxication include reduction in weight of liver, [20] vacuolation of hepatocytes, [21] and hepatic carcinoma. [22] Other liver lesions include enlargement of hepatic cells, fatty infiltration, necrosis, hemorrhage, fibrosis, regeneration of nodules, and bile duct proliferation/hyperplasia. [23]

Aspergillus flavus

Aspergillus flavus is a fungus of the family Trichocomaceae with a worldwide distribution. The mold lives in soil, surviving off dead plant and animal matter, but spreads through the air via airborne conidia. [24] This fungus grows in long branched hyphae and is capable of surviving on numerous food sources including corn and peanuts. [25] The fungus and its products are pathogenic to a number of species, including humans. [24] While toxicity of its products, aflatoxins, are explored throughout this article, Aspergillus flavus itself also exerts pathogenic effects through aspergillosis, or infection with the mold. This infection largely occurs in the lungs of immune compromised patients but infection may also occur in the skin or other organs. [26] Unlike many mold species, Aspergillus flavus prefers hot and dry conditions. Its optimal growth at 37 °C (99 °F) contributes to its pathogenicity in humans. [24]

Biosynthetic pathway

Aflatoxin B1 is derived from both a dedicated fatty acid synthase (FAS) and a polyketide synthase (PKS), together known as norsolorinic acid synthase. The biosynthesis begins with the synthesis of hexanoate by the FAS, which then becomes the starter unit for the iterative type I PKS. [27] [28] [29] The PKS adds seven malonyl-CoA extenders to the hexanoate to form the C20 polyketide compound. The PKS folds the polyketide in a particular way to induce cyclization to form the anthraquinone norsolorinic acid. A reductase then catalyzes the reduction of the ketone on the norsolorinic acid side-chain to yield averantin. [27] [28] [29] Averantin is converted to averufin via a two different enzymes, a hydroxylase and an alcohol dehydrogenase. This will oxygenate and cyclize averantin's side chain to form the ketal in averufin.

From this point on the biosynthetic pathway of aflatoxin B1 becomes much more complicated, with several major skeletal changes. Most of the enzymes have not been characterized and there may be several more intermediates that are still unknown. [27] However, what is known is that averufin is oxidized by a P450-oxidase, AvfA, in a Baeyer-Villiger oxidation. This opens the ether rings and upon rearrangement versiconal acetate is formed. Now an esterase, EstA, catalyzes the hydrolysis of the acetyl, forming the primary alcohol in versiconal. [27] [29] The acetal in versicolorin A is formed from the cyclization of the side-chain in versiconal, which is catalyzed by VERB synthase, and then VerB, a desaturase, reduces versicolorin B to form the dihydrobisfuran. [27] [29]

There are two more enzymes that catalyze the conversion of versicolorin A to demethylsterigmatocystin: AflN, an oxidase and AflM, a reductase. These enzymes use both molecular oxygen and two NADPH's to dehydrate one of the hydroxyl groups on the anthraquinone and open the quinine with the molecular oxygen. [27] [29] Upon forming the aldehyde in the ring opening step, it is oxidized to form the carboxylic acid and subsequently a decarboxylation event occurs to close the ring, forming the six-member ether ring system seen in demethylsterigmatocystin. The next two steps in the biosynthetic pathway is the methylation by S-adenosyl methionine (SAM) of the two hydroxyl groups on the xanthone part of demethysterigmatocystin by two different methyltransferases, OmtB and OmtA. [27] [29] This yields O-methylsterigmatocystin. In the final steps there is an oxidative cleavage of the aromatic ring and loss of one carbon in O-methylsterigmatocystin, which is catalyzed by OrdA, an oxidoreductase. [27] [29] Then a final recyclization occurs to form aflatoxin B1.

Mechanism of carcinogenicity

Aflatoxin B1 is a potent genotoxic hepatocarcinogen with its exposure strongly linked to the development of hepatocellular carcinoma, liver tumors, especially given co-infection with hepatitis B virus. [18] These effects seem to be largely mediated by mutations at guanine in codon 249 of the p53 gene, a tumor suppressing gene, [30] and at several guanine residues in the 12th and 13th codons of the ras gene, a gene whose product controls cellular proliferation signals. [31] [32] Aflatoxin B1 must first be metabolized into its reactive electrophilic form, aflatoxin B1-8,9-exo-epoxide by cytochrome p450. [18] This active form then intercalates between DNA base residues and forms adducts with guanine residues, most commonly aflatoxin B1-N7-Gua. These adducts may then rearrange or become removed from the backbone altogether, forming an apurinic site. These adducts and alterations represent lesions which, upon DNA replication cause the insertion of a mis-matched base in the opposing strand. Up to 44% of hepatocellular carcinomas in regions with high aflatoxin exposure bear a GC → TA transversion at codon 249 of p53, a characteristic mutation seen with this toxin. [32]

Adapted from Semela et al. 2001 Aflatoxin B1 DNA Adduct Formation.png
Adapted from Semela et al. 2001

Prevalence of hepatocellular carcinoma in individuals exposed to aflatoxin, increases with co-infection of hepatitis B virus. One study estimated that while individuals with urinary aflatoxin bio-markers were at a threefold greater risk than the normal population for hepatocellular carcinoma; those infected with hepatitis B virus were at a fourfold risk; and those with the aflatoxin bio-markers and infected with hepatitis B virus were at a 60 times greater risk for hepatocellular carcinoma than the normal population. [33] [32]

Toxicity

Several aflatoxin B1 toxicity studies have been conducted on various animal species. [34]

Acute toxicity
The oral LD50 range of aflatoxin B1 is estimated to be 0.3–17.9 mg/kg body weight for most animal species. [35] For instance, the oral LD50 of aflatoxin B1 is estimated to be 17.9 mg/kg body weight in female rats and 7.2 mg/kg body weight in male rats. Still in male rats, the intraperitoneal LD50 of aflatoxin B1 is estimated to be 6.0 mg/kg body weight. [36] Symptoms include anorexia, malaise, and low-grade fever. [37]
Subacute toxicity
Subacute toxicity studies of aflatoxin B1 in animals showed moderate to severe liver damage. In monkeys for instance, subacute toxicity studies showed portal inflammation and fatty change. [38]
Chronic toxicity
Chronic toxicity studies of aflatoxin B1 in chickens showed decreased hepatic microsomal cytochrome P-450 concentration, reduction in feed consumption and decreased weight gain. [39]
Subchronic toxicity
Subchronic toxicity studies of aflatoxin B1 in fish showed fish to present with preneoplastic lesions, concurrently with changes in gill, pancreas, intestine and spleen. [40]
Genotoxicity
Treatment of human liver cells with aflatoxin B1 at doses that ranged from 3–5 μmol/L resulted in the formation of aflatoxin B1-DNA adducts, 8-hydroxyguanine lesions and DNA damage. [41]
Carcinogenicity
The carcinogenicity of aflatoxin B1, which is characterized by the development of liver cell carcinoma, has been reported in rat studies. [42]
Embryotoxicity
Embryonic death and impaired embryonic development of the bursa of Fabricius in chickens by aflatoxin B1 has been reported. [43]
Teratogenicity
The teratogenic effects of aflatoxin B1 in rabbits have been reported to include reduced fetal weights, wrist drop, enlarged eye socket, agenesis of caudal vertebrae, micropthalmia, cardiac defects, and lenticular degeneration, among others. [44]
Immunotoxicity
Studies in fish showed aflatoxin B1 to have significant immunosuppressive effects including reduced serum total globulin and reduced bactericidal activities. [45]

Risk management and regulations

Aflatoxin B1 exposure is best managed by measures aimed at preventing contamination of crops in the field, post-harvest handling, and storage, or via measures aimed at detecting and decontaminating contaminated commodities or materials used in animal feed. For instance, biological decontamination involving the use of a single bacterial species, Flavobacterium aurantiacum has been used to remove aflatoxin B1 from peanuts and corn. [46]

Several countries around the world have rules and regulations governing aflatoxin B1 in foods and these include the maximum permitted, or recommended levels of aflatoxin B1 for certain foods. [47]

United States (US)
US food safety regulations have set a maximum permitted level of 20 μg/kg for aflatoxin B1, in combination with the other aflatoxins (B2, G1 and G2) in all foods, with the exception of milk which has a maximum permitted level of 0.5 μg/kg. Higher levels of 100–300 μg/kg are tolerable for some animal feeds. [48] [49]
European Union (EU)
The EU has set maximum permitted levels for aflatoxin B1 in nuts, dried fruits, cereals and spices to range from 2–12 μg/kg, while the maximum permitted level for aflatoxin B1 in infant foods is set at 0.1 μg/kg. [46] The maximum permitted levels for aflatoxin B1 in animal feeds set by the EU range from 5–50 μg/kg and these levels are much lower than those set in the US. [50]
Joint United Nations' Food and Agriculture Organization (FAO)/World Health Organization (WHO) Expert Committee on Food Additives (JECFA)
The FAO/WHO JECFA has set the maximum permitted levels of aflatoxin B1 in combination with the other aflatoxins (B2, G1 and G2) to be 15 μg/kg in raw peanuts and 10 μg/kg in processeds peanut; while the tolerance level of aflatoxin B1 alone is 5 μg/kg for dairy cattle feed. [51] [52]

Notable exposures

The discovery of aflatoxin B1 came on the heels of the widespread death of turkeys in England in the summer of 1960 to some unknown disease, at the time labeled "Disease X". Over the course of 500 outbreaks, the disease claimed over 100,000 turkeys which appeared to be healthy. The widespread death was later found to be caused by Aspergillus flavus contamination of peanut meal. [53] [54]

Twelve patients died of acute aflatoxin poisoning in several hospitals in the Machakos district of Kenya in 1981 following the consumption of contaminated maize. All patients also suffered from hepatitis. [55]

Following outbreaks of aflatoxin contamination in maize reaching 4,400 ppb in the spring of 2004, 125 individuals in Kenya died of acute hepatic failure while some 317 cases in total were reported. To date this was the largest known outbreak of aflatoxicosis in terms of fatalities documented. [37]

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<span class="mw-page-title-main">Mold health issues</span> Harmful effects of molds

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<span class="mw-page-title-main">Copra</span> Dried meat or kernel of the coconut

Copra is the dried, white flesh of the coconut from which coconut oil is extracted. Traditionally, the coconuts are sun-dried, especially for export, before the oil, also known as copra oil, is pressed out. The oil extracted from copra is rich in lauric acid, making it an important commodity in the preparation of lauryl alcohol, soaps, fatty acids, cosmetics, etc. and thus a lucrative product for many coconut-producing countries. The palatable oil cake, known as copra cake, obtained as a residue in the production of copra oil is used in animal feeds. The ground cake is known as coconut or copra meal.

<span class="mw-page-title-main">Aflatoxin</span> Group of poisons produced by moulds

Aflatoxins are various poisonous carcinogens and mutagens that are produced by certain molds, particularly Aspergillus species mainly by Aspergillus flavus and Aspergillus parasiticus. According to the USDA, "They are probably the best known and most intensively researched mycotoxins in the world." The fungi grow in soil, decaying vegetation and various staple foodstuffs and commodities such as hay, maize, peanuts, coffee, wheat, millet, sorghum, cassava, rice, chili peppers, cottonseed, tree nuts, sesame seeds, sunflower seeds, and various cereal grains and oil seeds. In short, the relevant fungi grow on almost any crop or food. When such contaminated food is processed or consumed, the aflatoxins enter the general food supply. They have been found in both pet and human foods, as well as in feedstocks for agricultural animals. Animals fed contaminated food can pass aflatoxin transformation products into milk, milk products, and meat. For example, contaminated poultry feed is the suspected source of aflatoxin-contaminated chicken meat and eggs in Pakistan.

<span class="mw-page-title-main">Hepatocellular carcinoma</span> Medical condition

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<i>Aspergillus flavus</i> Species of fungus

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<span class="mw-page-title-main">Fumonisin B1</span> Chemical compound

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<span class="mw-page-title-main">Sterigmatocystin</span> Chemical compound

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<i>Aspergillus parasiticus</i> Species of fungus

Aspergillus parasiticus is a fungus belonging to the genus Aspergillus. This species is an unspecialized saprophytic mold, mostly found outdoors in areas of rich soil with decaying plant material as well as in dry grain storage facilities. Often confused with the closely related species, A. flavus, A. parasiticus has defined morphological and molecular differences. Aspergillus parasiticus is one of three fungi able to produce the mycotoxin, aflatoxin, one of the most carcinogenic naturally occurring substances. Environmental stress can upregulate aflatoxin production by the fungus, which can occur when the fungus is growing on plants that become damaged due to exposure to poor weather conditions, during drought, by insects, or by birds. In humans, exposure to A. parasiticus toxins can cause delayed development in children and produce serious liver diseases and/or hepatic carcinoma in adults. The fungus can also cause the infection known as aspergillosis in humans and other animals. A. parasiticus is of agricultural importance due to its ability to cause disease in corn, peanut, and cottonseed.

<span class="mw-page-title-main">Nivalenol</span> Type of mycotoxin

Nivalenol (NIV) is a mycotoxin of the trichothecene group. In nature it is mainly found in fungi of the Fusarium species. The Fusarium species belongs to the most prevalent mycotoxin producing fungi in the temperate regions of the northern hemisphere, therefore making them a considerable risk for the food crop production industry.

Aflatoxin M<sub>1</sub> Chemical compound

Aflatoxin M1 is a chemical compound of the aflatoxin class, a group of mycotoxins produced by three species of Aspergillus – Aspergillus flavus, Aspergillus parasiticus, and the rare Aspergillus nomius – which contaminate plant and plant products.

References

  1. ↑ "AFLATC: Aflatoxin B1 chloroform solvate". Cambridge Structural Database: Access Structures. Cambridge Crystallographic Data Centre . Retrieved 2021-03-23.
  2. ↑ van Soest TC, Peerdeman AF (1970). "The crystal structures of aflatoxin B1. I. The structure of the chloroform solvate of aflatoxin B1 and the absolute configuration of aflatoxin B1". Acta Crystallogr. B . 26 (12): 1940–1947. Bibcode:1970AcCrB..26.1940V. doi:10.1107/S0567740870005228.
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  9. ↑ Chen T, Liu J, Li Y, Wei S (February 2022). "Burden of Disease Associated with Dietary Exposure to Aflatoxins in China in 2020". Nutrients. 14 (5): 1027. doi: 10.3390/nu14051027 . PMC   8912679 . PMID   35268003.
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  17. ↑ Viegas S, Veiga L, Malta-Vacas J, Sabino R, Figueredo P, Almeida A, et al. (2012). "Occupational exposure to aflatoxin (AFB₁) in poultry production". Journal of Toxicology and Environmental Health. Part A. 75 (22–23): 1330–1340. Bibcode:2012JTEHA..75.1330V. doi:10.1080/15287394.2012.721164. hdl: 10400.21/1927 . PMID   23095151. S2CID   205866375.
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  19. ↑ Boonen J, Malysheva SV, Taevernier L, Diana Di Mavungu J, De Saeger S, De Spiegeleer B (November 2012). "Human skin penetration of selected model mycotoxins". Toxicology. 301 (1–3): 21–32. Bibcode:2012Toxgy.301...21B. doi:10.1016/j.tox.2012.06.012. PMID   22749975.
  20. ↑ Fernández A, Ramos JJ, Sanz M, Saez T, Fernández de Luco D (1996). "Alterations in the performance, haematology and clinical biochemistry of growing lambs fed with aflatoxin in the diet". Journal of Applied Toxicology. 16 (1): 85–91. doi:10.1002/(SICI)1099-1263(199601)16:1<85::AID-JAT315>3.0.CO;2-T. PMID   8821681.
  21. ↑ Espada Y, Domingo M, Gomez J, Calvo MA (November 1992). "Pathological lesions following an experimental intoxication with aflatoxin B1 in broiler chickens". Research in Veterinary Science. 53 (3): 275–9. doi:10.1016/0034-5288(92)90127-n. PMID   1465501.
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