Clostridium perfringens

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Clostridium perfringens
Clostridium perfringens.jpg
Photomicrograph of Gram-positive Clostridium perfringens bacilli
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Bacteria
Phylum: Bacillota
Class: Clostridia
Order: Eubacteriales
Family: Clostridiaceae
Genus: Clostridium
Species:
C. perfringens
Binomial name
Clostridium perfringens
Veillon & Zuber 1898
Hauduroy et al. 1937

Clostridium perfringens (formerly known as C. welchii , or Bacillus welchii) is a Gram-positive, bacillus (rod-shaped), anaerobic, spore-forming pathogenic bacterium of the genus Clostridium . [1] [2] C. perfringens is ever-present in nature and can be found as a normal component of decaying vegetation, marine sediment, the intestinal tract of humans and other vertebrates, insects, and soil. It has the shortest reported generation time of any organism at 6.3 minutes in thioglycolate medium. [3]

Contents

Clostridium perfringens is one of the most common causes of food poisoning in the United States, alongside norovirus, Salmonella , Campylobacter , and Staphylococcus aureus . [4] However, it can sometimes be ingested and cause no harm. [5]

Infections due to C. perfringens show evidence of tissue necrosis, bacteremia, emphysematous cholecystitis, and gas gangrene, also known as clostridial myonecrosis. The specific name, perfringens, is derived from the Latin per (meaning "through") and frango ("burst"), referring to the disruption of tissue that occurs during gas gangrene. [6] The toxin involved in gas gangrene is α-toxin, which inserts into the plasma membrane of cells, producing gaps in the membrane that disrupt normal cellular function. C. perfringens can participate in polymicrobial anaerobic infections. It is commonly encountered in infections as a component of the normal flora. In this case, its role in disease is minor.

C. perfringens toxins are a result of horizontal gene transfer of a neighboring cell's plasmids. [7] Shifts in genomic make-up are common for this species of bacterium and contribute to novel pathogensis. [8] Major toxins are expressed differently in certain populations of C. perfringens; these populations are organized into strains based on their expressed toxins. [9] This especially impacts the food industry, as controlling this microbe is important for preventing foodborne illness. [8] Novel findings in C. perfringens hyper-motility, which was provisionally thought as non-motile, have been discovered as well. [10] Findings in metabolic processes reveal more information concerning C. perfringens pathogenic nature. [11]

Genome

Clostridium perfringens has a stable G+C content around 27 to 28 percent and average genome size of 3.5 Mb. [12] Genomes of 56 C. perfringens strains have since been made available on the NCBI genomes database for the scientific research community. Genomic research has revealed surprisingly high diversity in C. perfringens pangenome, with only 12.6 percent core genes, identified as the most divergent Gram-positive bacteria reported. [12] Nevertheless, 16S rRNA regions in between C. perfringens strains are found to be highly conserved (sequence identity >99.1%). [12]

The Clostridium perfringens enterotoxin (CPE)–producing strain has been identified to be a small portion of the overall C. perfringens population (~1-5%) through genomic testing. [13] Advances in genetic information surrounding strain A CPE C. perfringens has allowed techniques such as microbial source tracking (MST) to identify food contamination sources. [13] The CPE gene has been found within chromosomal DNA as well as plasmid DNA. Plasmid DNA has been shown to play and integral role in cell pathogenesis and encodes for major toxins, including CPE. [7]

C. perfringens has been shown to carry plasmid-containing genes for antibiotic resistance. The pCW3 plasmid is the primary conjugation plasmid responsible for creating antibiotic resistance in C. perfringens. Furthermore, the pCW3 plasmid also encodes for multiple toxins found in pathogenic strains of C. perfringens. [14] Antibiotic resistance genes observed thus far include tetracycline resistance, efflux protein, and aminoglycoside resistance. [15]

Within industrial contexts, such as food production, sequencing genomes for pathogenic strains of C. perfringens has become an expanding field of research. Poultry production is impacted directly from this trend as antibiotic-resistant strains of C. perfringens are becoming more common. [8] By performing a meta-genome analysis, researches are capable to identify novel strains of pathogenic bacterium, such as C. perfringens B20. [8]

Motility

Clostridium perfringens is provisionally identified as non-motile. They lack flagella; however, recent research suggests gliding as a form of motility. [16] [17]

Hyper-motile variations

This illustration depicts a three-dimensional (3D), computer-generated image of a cluster of barrel-shaped, Clostridium perfringens bacteria. The artistic recreation was based upon scanning electron microscopic (SEM) imagery. 21913 lores.jpg
This illustration depicts a three-dimensional (3D), computer-generated image of a cluster of barrel-shaped, Clostridium perfringens bacteria. The artistic recreation was based upon scanning electron microscopic (SEM) imagery.

In agar plate cultures bacteria with hypermotile variations like SM101 frequently appear around the borders of the colonies. They create long thin filaments that enable them to move quickly, much like bacteria with flagella, according to video imaging of their gliding motion. The causes of the hypermotile phenotype and its immediate descendants were found using genome sequencing. The hypermotile offspring of strains SM101 and SM102, SM124 and SM127, respectively, had 10 and 6 nucleotide polymorphisms (SNPs) in comparison to their parent strains. The hypermotile strains have the common trait of gene mutations related to cell division. [16]

Regulation of gliding motility: The CpAL/VirSR system

Some strains of C. perfringens cause various diseases like gas gangrene and myonecrosis. Toxins produced that are required for myonecrosis is regulated by the C. perfringens Agr-like (CpAl) system through the VirSR two-component system. The CpAL/VirSR system is a quorum sensing system encoded by other pathogenic clostridia. Myonecrosis starts at the infection site and involves bacteria migrating deeper via gliding motility. Researchers investigated if the CpAL/VirSR system regulates gliding motility. The study demonstrated that the CpAL/VirSR regulates C. perfringensgliding motility. Additionally, the study suggests that gliding bacteria in myonecrosis have increased transcription of toxin genes. [17]

Transformation

There are two methods of genetic manipulation via experimentation that have been shown to cause genetic transformation in C. perfringens.

Protoplast transformation

The first report of transformation in C. perfringens involved polyethylene glycol-mediated transformation of protoplasts. The transformation procedure involved the addition of the plasmid DNA to the protoplasts in the presence of high concentrations of polyethylene glycol. During the first protoplast transformation experiment, L-phase variants of C. perfringens were generated by penicillin treatment in the presence 0.4m sucrose. After the transformation procedure was completed, all of the transformed cells were still in the form of L-phase variants. Reversion to vegetative cells was not obtained, but it was observed that autoplasts (protoplasts derived from autolysis) were able to be regenerated to produce rods with cell walls and could be transformed with C. perfringens plasmid DNA. [18]

Electroporation

Electroporation involves the application of a high-voltage electric field to vegetative bacteria cells for a very short period. This technique resulted in major advances in genetic transformation of C. perfringens, due to the bacteria often displaying itself as a vegetative cell or as dormant spores in food. [19] The electric pulse creates pores in the bacterial cell membrane and allows the passive influx of DNA molecules. [20]

Transmission and pathogenesis

C. perfringens is most commonly known for foodborne illness, but can translocate from a gastrointestinal source into the bloodstream which causes bacteremia. C. perfringens bacteremia can lead to toxin-mediated intravascular hemolysis and septic shock. [21] This is rare as it makes up less than 1% of bloodstream isolates, but is highly fatal with a reported mortality rate of 27% to 58%. [22]

Clostridium perfringens is the most common bacterial agent for gas gangrene. Some symptoms include blisters, tachycardia, swelling, and jaundice. [23]

A strain of C. perfringens might be implicated in multiple sclerosis (MS) nascent (Pattern III) lesions. [24] Tests in mice found that a two strains of intestinal C. perfringens that produced epsilon toxins (ETX) caused MS-like damage in the brain, and earlier work had identified this strain of C. perfringens in a human with MS. [25] [26] MS patients were found to be 10 times more immune-reactive to the epsilon toxin than healthy people. [27]

Perfringolysin O (pfoA)-positive C. perfringens strains were also associated with the rapid onset of necrotizing enterocolitis in preterm infants. [28]

Metabolic processes

C. perfringens is an aerotolerant anaerobe bacterium that lives in a variety of environments including soil and human intestinal tract. [11] C. perfringens is incapable of synthesizing multiple amino acids due to the lack of genes required for biosynthesis. [11] Instead, the bacterium produces enzymes and toxins to break down host cells and import nutrients from the degrading cell. [11]

C. perfringens has a complete set of enzymes for glycolysis and glycogen metabolism. In the fermentation pathway, pyruvate is converted into acetyl-CoA by pyruvate-ferredoxin oxidoreductase, producing CO2 gas and reduced ferredoxin. [29] Electrons from the reduced ferredoxin are transferred to protons by hydrogenase, resulting in the formation of hydrogen molecules (H2) that are released from the cell along with CO2. Pyruvate is also converted to lactate by lactate dehydrogenase, whereas acetyl-CoA is converted into ethanol, acetate, and butyrate through various enzymatic reactions, completing the anaerobic glycolysis that serves as a potential main energy source for C. perfringens. C. perfringens utilizes a variety of sugars such as fructose, galactose, glycogen, lactose, maltose, mannose, raffinose, starch, and sucrose, and various genes for glycolytic enzymes. The amino acids of these various enzymes and sugar molecules are converted to propionate through propionyl-CoA, which results in energy production. [29]

Infection

Infections due to C. perfringens show evidence of tissue necrosis, bacteremia, emphysematous cholecystitis, and gas gangrene, also known as clostridial myonecrosis. The toxin involved in gas gangrene is α-toxin, which inserts into the plasma membrane of cells, producing gaps in the membrane that disrupt normal cellular function. C. perfringens can participate in polymicrobial anaerobic infections.[ citation needed ]

Clostridium perfringens food poisoning can also lead to another disease known as enteritis necroticans or clostridial necrotizing enteritis, (also known as pigbel); this is caused by C. perfringens type C. This infection is often fatal. Large numbers of C. perfringens grow in the intestines, and secrete exotoxin. This exotoxin causes necrosis of the intestines, varying levels of hemorrhaging, and perforation of the intestine. Inflammation usually occurs in sections of the jejunum, midsection of the small intestine. This disease eventually leads to septic shock and death. This particular disease is rare in the United States; typically, it occurs in populations with a higher risk. Risk factors for enteritis necroticans include protein-deficient diet, unhygienic food preparation, sporadic feasts of meat (after long periods of a protein-deficient diet), diets containing large amounts of trypsin inhibitors (sweet potatoes), and areas prone to infection of the parasite Ascaris (produces a trypsin inhibitor). This disease is contracted in populations living in New Guinea, parts of Africa, Central America, South America, and Asia. [30]

Tissue gas occurs when C. perfringens infects corpses. It causes extremely accelerated decomposition, and can only be stopped by embalming the corpse. Tissue gas most commonly occurs to those who have died from gangrene, large decubitus ulcers, necrotizing fasciitis or to those who had soil, feces, or water contaminated with C. perfrigens forced into an open wound. [31] These bacteria are resistant to the presence of formaldehyde in normal concentrations.[ citation needed ]

Food poisoning

C. perfringens forms spores that are distributed through air, soil, and water. The most common cause of illness comes from the ingestion of poorly cooked meats that are contaminated by these spores. [32] After this meat is left out at 20 °C to 60 °C, the spores germinate and C. perfringens then grows rapidly. The bacteria produce a toxin that causes diarrhea. [33]

Food poisoning in humans is caused by type A strains able to produce C. perfringens enterotoxin. [34] This enterotoxin is a polypeptide of 35.5 kDa that accumulates in the beginning of the sporulation, and is excreted to the media when it lysates at the end of the sporulation. It is coded by the cpe gene, which is present in less than 5% of the type A strains, and it can be located in the chromosome or in an external plasmid [35]

In the United Kingdom and United States, C. perfringens bacteria are the third-most common cause of foodborne illness, with poorly prepared meat and poultry, or food properly prepared, but left to stand too long, the main culprits in harboring the bacterium. [36] The C. perfringens enterotoxin that mediates the disease is heat-labile (inactivated at 74 °C (165 °F)). It can be detected in contaminated food (if not heated properly), and feces. [37] Incubation time is between 6 and 25 (commonly 10–12) hours after ingestion of contaminated food. [38]

Since C. perfringens forms spores that can withstand cooking temperatures, if cooked food is left standing for long enough, germination can ensue and infective bacterial colonies develop. Symptoms typically include abdominal cramping, diarrhea, and fever. [30] The whole course usually resolves within 24 hours, but can last up to 2 weeks in older or infirm hosts. [39] Despite its potential dangers, C. perfringens is used as the leavening agent in salt-rising bread. The baking process is thought to reduce the bacterial contamination, precluding negative effects. [5]

Many cases of C. perfringens food poisoning likely remain subclinical, as antibodies to the toxin are common among the population. This has led to the conclusion that most of the population has experienced food poisoning due to C. perfringens.[ citation needed ]

Virulence

Membrane-damaging enzymes, pore-forming toxins, intracellular toxins, and hydrolytic enzymes are the functional categories into which C. perfringens' virulence factors may be divided. These virulence factor-encoding genes can be found on chromosomes and large plasmids. [9]

Enzymes

The human gastrointestinal tract is lined with a protective layer called the intestinal mucosa. The function of intestinal mucosa is to secrete mucus and act as a defense mechanism against pathogens, toxins, and harmful substances. Within the mucus is mucin, an O-glycan glycoprotein that recognizes and forms a barrier around invaders and prevents them from attaching to endothelial cells and infecting them. [40] [41] C. perfringens can secrete different carbohydrate-active enzymes used to degrade mucins and other O-glycans within the intestinal mucosa. These enzymes include: Sialidase, Hexosaminidase, Galactosidase, Fucosidase, and Complementary enzymes belonging to various glycoside hydrolase families. [41]

Sialidase

Sialidases, also called neuraminidases, function to breakdown mucin by hydrolyzing the terminal sialic acid residues located within the protein through the process of desialylation. C. perfringens has three sialidases belonging to glycoside hydrolase family 33 (GH33): NanH, NanI, and NanJ. [41]

C. perfringens secretes and releases NanI and NanJ, but NanH does not have a secretion signal peptide and is believed to operate in the cytoplasm. NanH has only one catalytic domain, while NanI and NanJ contain additional carbohydrate-binding modules (CBMs). NanI contains an N-terminal CBM40, whereas NanJ has an N-terminal CBM40 as well as an N-terminal CBM32. [41]

Studies on the three-dimensional structure of NanI show that there is an active site with a pocket-like shape, allowing for the removal of sialic acid residues from sialomucins in the intestinal mucosa. [41]

Hexosaminidase

The mucus layer consists of intestinal mucin glycans, glycolipids, and glycoproteins that contain hexosamines, such as N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc). C. perfringens encodes for eight hexosaminidases that break down hexosamines in the mucus. These hexosaminidases belong to four glycoside hydrolase families: GH36, GH84, GH89, and GH123. [41]

C. perfringens has two hexosaminidases in GH36: AagA (CpGH36A), which removes GalNAc from O-glycans, and the predicted CpGH36B, whose exact function is still unknown but is expected to have a similar structure. [41]

NagH, NagI, NagJ, and NagK belong to GH84. They are known to cleave terminal GlcNAc residues using a specific mechanism. [41]

AgnC (CpGH89), belonging to GH89, releases N-acetyl-D-glucosamine from class III mucins and can act on gastric mucin. [41]

CpNga123, belonging to GH123, cleaves β-D-GalNAc found in glycosphingolipids. Unlike the other enzymes, it does not have a signal for secretion and is thought to work on glycans taken up into the bacterial cell. [41]

Galactosidase

C. perfringens has four galactosidases that belong to the glycoside hydrolase family 2 (GH2): CpGH2A, CpGH2B, CpGH2C, and CpGH2D. Research suggests that these enzymes are effective at breaking down core mucin glycan structures. However, as research is limited on galactosidases in C. perfringens, the exact functioning of these enzymes are unknown. [41]

Fucosidase

Fucose monosaccharides are located on the terminal ends of core O-linked glycans. Clostridium perfringens has three α-L-fucosidases belonging to two different families: Afc1 (GH29), Afc2 (GH29), and Afc3 (GH95). These fucosyl residues typically cap the ends of glycans, providing protection against enzymatic digestion. [41]

Major toxins

There are five major toxins produced by Clostridium perfringens. Alpha, beta, epsilon and enterotoxin are toxins that increase a cells permeability which causes an ion imbalance while iota toxins destroy the cell's actin cytoskeleton. [42] On the basis of which major, "typing" toxins are produced, C. perfringens can be classified into seven "toxinotypes", A, B, C, D, E, F and G: [43]

Toxinotypes of C. perfringens [43] : fig.1  [44]
Toxin
Type
AlphaBetaEpsilonIotaEnterotoxinNetBNotes
A+-----
B+++---
C++--+/--
D+-+-+/--
E+--++/--
F+---+-
G+----+

Alpha toxin

Alpha toxin (CPA) is a zinc-containing phospholipase C, composed of two structural domains, which destroy a cell's membrane. Alpha toxins are produced by all five types of C. perfringens. This toxin is linked to gas gangrene of humans and animals. Most cases of gas gangrene has been related to a deep wound being contaminated by soil that harbors C. perfringens. [42] [45]

Beta toxin

Beta toxins (CPB) are a protein that causes hemorrhagic necrotizing enteritis and enterotoxaemia in both animals (type B) and humans (type C) which leads to the infected individual's feces becoming bloody and their intestines necrotizing. [42] Proteolytic enzymes, such as trypsin, can break down CPB, making them ineffective. Therefore, the presence of trypsin inhibitors in colostrum makes CPB especially deadly for mammal offspring. [46]

Epsilon toxin

Epsilon toxin (ETX) is a protein produced by type B and type D strains of C. perfringens. This toxin is currently ranked the third most potent bacterial toxin known. [47] ETX causes enterotoxaemia in mainly goats and sheep, but cattle are sometime susceptible to it as well. An experiment using mice found that ETX had an LD50 of 50-110 ng/kg. [48] The excessive production of ETX increases the permeability of the intestines. This causes severe edema in organs such as the brain and kidneys. [49]

The very low LD50 of ETX has led to concern that it may be used as a bioweapon. It appeared on the select agent lists of the US CDC and USDA, until it was removed in 2012. There are no human vaccines for this toxin, but effective vaccines for animals exist. [50]

Iota toxin

Iota toxin (ITX) is a protein produced by type E strains of C. perfringens. Iota toxins are made up of two, unlinked proteins that form a multimeric complex on cells. Iota toxins prevent the formation of filamentous actin. This causes the destruction of the cells cytoskeleton which in turn leads to the death of the cell as it can no longer maintain homeostasis. [51]

Enterotoxin

This toxin (CPE) causes food poisoning. It alters intracellular claudin tight junctions in gut epithelial cells. This pore-forming toxin also can bind to human ileal and colonic epithelium in vitro and necrotize it. Through the caspase-3 pathway, this toxin can cause apoptosis of affected cells. This toxin is linked to type F strains, but has also been found to be produced by certain types of C, D, and E strains. [52]

Other toxins

TpeL is a toxin found in type B, C, and G [53] strains. It is in the same protein family as C. difficile toxin A. [54] It does not appear important in the pathogenesis of types B and C infections, but may contribute to virulence in type G strains. It glycosylates Rho and Ras GTPases, disrupting host cell signaling. [53]

Clinical manifestations and diagnosis

Clostridium perfringens infections can lead to a variety of clinical manifestations, ranging from mild gastrointestinal symptoms to life-threatening conditions. The most common presentation is food poisoning, characterized by acute abdominal pain, diarrhea, and, in some cases, vomiting, typically occurring 6 to 24 hours after ingestion of contaminated food. [55] In more severe cases, particularly with the C. perfringens type A strain, individuals may develop necrotizing enteritis, which can cause severe abdominal pain, fever, and systemic symptoms due to intestinal necrosis. [56] Additionally, C. perfringens can be implicated in gas gangrene, a rapidly progressing soft tissue infection that presents with severe pain, swelling, and the presence of gas in tissues. [57] These varied clinical manifestations highlight the importance of prompt diagnosis and treatment to mitigate severe outcomes associated with C. perfringens infections.

In the diagnosis of Clostridium perfringens, Nagler's reaction serves as a useful test. This method involves culturing the organism on an egg yolk agar plate, where one side is treated with anti-alpha-toxin while the other side remains untreated. When a streak of the suspect organism is applied across both sides, turbidity forms around the side lacking the anti-alpha-toxin. This turbidity indicates uninhibited lecithinase activity, a hallmark of C. perfringens due to its production of alpha-toxin. This reaction effectively differentiates C. perfringens from other bacterial species. [58]

Typically, the symptoms of C. perfringens poisoning are used to diagnose it. However, diagnosis can be made using a stool culture test, in which the feces are tested for toxins produced by the bacteria. [59]

Prevention

Most foods, notably beef and chicken, can be prevented from growing C. perfringens spores by cooking them to the necessary internal temperatures. The best way to check internal temperatures is by using kitchen thermometers. [39] The temperature that C. perfringens can multiply within can range anywhere from 59 °F (15 °C) to 122 °F (50 °C). [60] After two hours of preparation, leftover food should be chilled to a temperature of less than 40 °F (4 °C). Large pots of soup or stew that contain meats should be split into smaller portions and refrigerated with a lid on. Before serving, leftovers must be warmed to at least 165 °F (74 °C). As a general rule, food should be avoided if it tastes, smells, or appears differently than it should. Food that has been out for a long period might also be unsafe to eat, even if it appears healthy. [39]

Treatment

The most important aspect of treatment is prompt and extensive surgical debridement of the involved area and excision of all devitalized tissue, in which the organisms are prone to grow. Administration of antimicrobial drugs, particularly penicillin, is begun at the same time. Clostridium perfringens is more often susceptible to vancomycin compared to other pathogenic Clostridia and 20% of the strains are resistant to clindamycin. [61] Metronidazole resistance is also relatively common, with a resistance rate of 10%. [62] Hyperbaric oxygen may be of help in the medical management of clostridial tissue infections. [63] Most people who suffer from food poisoning caused by C. perfringens tend to fight off the illness without the need of any antibiotics. Extra fluids should be drank consistently until diarrhea dissipates. [64]

Epidemiology

Clostridium perfringens is a leading cause of food poisoning in the United States and Canada. [65] Contaminated meats in stews, soups, and gravies are usually responsible for outbreaks and cause about 1 million cases of foodborne illnesses in the United States every year. [64] Deaths due to the disease are rare and mostly occur in elderly and people who are predisposed to the disease. [66] From 1998 to 2010, 289 confirmed outbreaks of C. perfringens illness were reported with 15,208 illnesses, 82 hospitalizations, and eight deaths. [67]

Food poisoning incidents

On May 7, 2010, 42 residents and 12 staff members at a Louisiana (USA) state psychiatric hospital were affected and experienced vomiting, abdominal cramps, and diarrhea. Three patients died within 24 hours. The outbreak was linked to chicken which was cooked a day before it was served and was not cooled down according to hospital guidelines. The outbreak affected 31% of the residents of the hospital and 69% of the staff who ate the chicken. How many of the affected residents ate the chicken is unknown. [68]

In May 2011, a man died after allegedly eating food contaminated with the bacteria on a transatlantic American Airlines flight. The man's wife and daughter were suing American and LSG Sky Chefs, the German company that prepared the inflight food. [69]

In December 2012, a 46-year-old woman died two days after eating a Christmas Day meal at a pub in Hornchurch, Essex, England. She was among about 30 people to fall ill after eating the meal. Samples taken from the victims contained C. perfringens. The hotel manager and the cook were jailed for offences arising from the incident. [70]

In December 2014, 87-year-old Bessie Scott died three days after eating a church potluck supper in Nackawic, New Brunswick, Canada. Over 30 other people reported signs of gastrointestinal illness, diarrhea, and abdominal pain. The province's acting chief medical officer says, Clostridium perfringens is the bacteria [sic] that most likely caused the woman's death. [71]

In October 2016, 66-year-old Alex Zdravich died four days after eating an enchilada, burrito, and taco at Agave Azul in West Lafayette, Indiana, United States. Three others who dined the same day reported signs of foodborne illness, which were consistent with the symptoms and rapid onset of C. perfringens infection. They later tested positive for the presence of the bacteria, but the leftover food brought home by Zdravich tested negative. [72] [73]

In November 2016, food contaminated with C. perfringens caused three individuals to die, and another 22 to be sickened, after a Thanksgiving luncheon hosted by a church in Antioch, California, United States. [74]

In January 2017, a mother and her son sued a restaurant in Rochester, New York, United States, as they and 260 other people were sickened after eating foods contaminated with C. perfringens. "Officials from the Monroe County Department of Public Health closed down the Golden Ponds after more than a fourth of its Thanksgiving Day guests became ill. An inspection revealed a walk-in refrigerator with food spills and mold, a damaged gasket preventing the door from closing, and mildew growing inside." [75]

In July 2018, 647 people reported symptoms after eating at a Chipotle Mexican Grill restaurant in Powell, Ohio, United States. Stool samples tested by the CDC tested positive for C. perfringens. [76]

In November 2018, approximately 300 people in Concord, North Carolina, United States, were sickened by food at a church barbecue that tested positive for C. perfringens. [77]

In 2021, dozens of hospital workers in Alaska were sick and it was traced back to a Cubano Sandwich. Health officials wrote that almost all symptoms resolved within 24 hours. No one who ate the food reportedly needed hospitalization. It is rare for Alaska to see an outbreak with this magnitude when it’s not associated with some sort of national food borne illness. [78]

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Clostridium perfringens alpha toxin is a toxin produced by the bacterium Clostridium perfringens and is responsible for gas gangrene and myonecrosis in infected tissues. The toxin also possesses hemolytic activity.

<i>Clostridium tetani</i> Common soil bacterium and the causative agent of tetanus

Clostridium tetani is a common soil bacterium and the causative agent of tetanus. Vegetative cells of Clostridium tetani are usually rod-shaped and up to 2.5 μm long, but they become enlarged and tennis racket- or drumstick-shaped when forming spores. C. tetani spores are extremely hardy and can be found globally in soil or in the gastrointestinal tract of animals. If inoculated into a wound, C. tetani can grow and produce a potent toxin, tetanospasmin, which interferes with motor neurons, causing tetanus. The toxin's action can be prevented with tetanus toxoid vaccines, which are often administered to children worldwide.

<i>Clostridium septicum</i> Species of bacterium

Clostridium septicum is a gram positive, spore forming, obligate anaerobic bacterium.

Microbial toxins are toxins produced by micro-organisms, including bacteria, fungi, protozoa, dinoflagellates, and viruses. Many microbial toxins promote infection and disease by directly damaging host tissues and by disabling the immune system. Endotoxins most commonly refer to the lipopolysaccharide (LPS) or lipooligosaccharide (LOS) that are in the outer plasma membrane of Gram-negative bacteria. The botulinum toxin, which is primarily produced by Clostridium botulinum and less frequently by other Clostridium species, is the most toxic substance known in the world. However, microbial toxins also have important uses in medical science and research. Currently, new methods of detecting bacterial toxins are being developed to better isolate and understand these toxins. Potential applications of toxin research include combating microbial virulence, the development of novel anticancer drugs and other medicines, and the use of toxins as tools in neurobiology and cellular biology.

Clostridium novyi (oedematiens) a Gram-positive, endospore- forming, obligate anaerobic bacteria of the class Clostridia. It is ubiquitous, being found in the soil and faeces. It is pathogenic, causing a wide variety of diseases in humans and animals.

Clostridium perfringens beta toxin is one of the four major lethal protein toxins produced by Clostridium perfringens Type B and Type C strains. It is a necrotizing agent and it induces hypertension by release of catecholamine. It has been shown to cause necrotic enteritis in mammals and induces necrotizing intestinal lesions in the rabbit ileal loop model. C. perfringens beta toxin is susceptible to breakdown by proteolytic enzymes, particularly trypsin. Beta toxin is therefore highly lethal to infant mammals because of trypsin inhibitors present in the colostrum.

Hathewaya histolytica is a species of bacteria found in feces and the soil. It is a motile, gram-positive, aerotolerant anaerobe. H. histolytica is pathogenic in many species, including guinea pigs, mice, and rabbits, and humans. H. histolytica has been shown to cause gas gangrene, often in association with other bacteria species.

<i>Clostridium tertium</i> Species of bacterium

Clostridium tertium is an anaerobic, motile, gram-positive bacterium. Although it can be considered an uncommon pathogen in humans, there has been substantial evidence of septic episodes in human beings. C. tertium is easily decolorized in Gram-stained smears and can be mistaken for a Gram-negative organism. However, C.tertium does not grow on selective media for Gram-negative organisms.

In molecular biology, VR-RNA is a small RNA produced by Clostridium perfringens. It functions as a regulator of the two-component VirR/VirS system.

<i>Clostridioides difficile</i> Species of bacteria

Clostridioides difficile is a bacterium known for causing serious diarrheal infections, and may also cause colon cancer. It is known also as C. difficile, or C. diff, and is a Gram-positive species of spore-forming bacteria. Clostridioides spp. are anaerobic, motile bacteria, ubiquitous in nature and especially prevalent in soil. Its vegetative cells are rod-shaped, pleomorphic, and occur in pairs or short chains. Under the microscope, they appear as long, irregular cells with a bulge at their terminal ends. Under Gram staining, C. difficile cells are Gram-positive and show optimum growth on blood agar at human body temperatures in the absence of oxygen. C. difficile is catalase- and superoxide dismutase-negative, and produces up to three types of toxins: enterotoxin A, cytotoxin B and Clostridioides difficile transferase. Under stress conditions, the bacteria produce spores that are able to tolerate extreme conditions that the active bacteria cannot tolerate.

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