Route 140 or Highway 140 may refer to:
Japan National Route 140 
Fukuoka Prefectural Route 140
Nara Prefectural Route 140
A140 road
Interstate 140 (two current highways)
U.S. Route 140 (former)
Alabama State Route 140 
Arkansas Highway 140 
California State Route 140 
Colorado State Highway 140 
Connecticut Route 140 
Georgia State Route 140 
Illinois Route 140 
Indiana State Road 140 
Iowa Highway 140 
K-140 (Kansas highway) 
Kentucky Route 140 
Louisiana Highway 140 
Maine State Route 140 
Maryland Route 140 
Massachusetts Route 140 
M-140 (Michigan highway) 
Nevada State Route 140 
New Hampshire Route 140 
New Jersey Route 140 
New Mexico State Road 140 
New York State Route 140 
County Route 140 (Broome County, New York) 
County Route 140 (Erie County, New York) 
County Route 140 (Fulton County, New York) County Route 140 (Montgomery County, New York) County Route 140 (Niagara County, New York) County Route 140 (Onondaga County, New York) 
County Route 140 (Rensselaer County, New York) 
County Route 140 (Wayne County, New York) 
North Carolina Highway 140 
Ohio State Route 140 
Oregon Route 140 
Tennessee State Route 140 
Texas State Highway 140 
Utah State Route 140 
Utah State Route 140 (pre-1969) (former)
Vermont Route 140 
Virginia State Route 140 Virginia State Route 140 (pre-1933) (former) Virginia State Route 140 (1933-1949) (former)
Washington State Route 140 (former)
West Virginia Route 140 
Wisconsin Highway 140 | Preceded by 139 | Lists of highways 140 | Succeeded by 141 |
