Protein kinase, AMP-activated, alpha 1

PRKAA1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4RED, 4RER, 4REW, 5EZV
Identifiers
Aliases	PRKAA1 , AMPK, AMPKa1, Protein kinase, AMP-activated, alpha 1, protein kinase AMP-activated catalytic subunit alpha 1
External IDs	OMIM: 602739; MGI: 2145955; HomoloGene: 49590; GeneCards: PRKAA1; OMA:PRKAA1 - orthologs
EC number	2.7.11.26
Gene location (Human) Chr. Chromosome 5 (human) [1] Band n/a Start 40,759,379 bp [1] End 40,798,374 bp [1]
Gene location (Mouse) Chr. Chromosome 15 (mouse) [2] Band 15|15 A1 Start 5,173,343 bp [2] End 5,211,380 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Achilles tendon sperm rectum gallbladder gastric mucosa stromal cell of endometrium epithelium of colon tibial arteries body of pancreas Descending thoracic aorta Top expressed in granulocyte islet of Langerhans epithelium of small intestine mammillary body white adipose tissue hand ventromedial nucleus medial vestibular nucleus lateral hypothalamus ventral tegmental area More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity nucleotide binding protein kinase activity cAMP-dependent protein kinase activity [acetyl-CoA carboxylase kinase activity] AMP-activated protein kinase activity histone serine kinase activity chromatin binding metal ion binding kinase activity protein serine/threonine kinase activity tau-protein kinase activity protein C-terminus binding protein binding [hydroxymethylglutaryl-CoA reductase (NADPH) kinase activity] ATP binding tau protein binding Cellular component nucleotide-activated protein kinase complex apical plasma membrane nucleoplasm nucleus cytoplasm cytosol nuclear speck intracellular anatomical structure axon dendrite protein-containing complex soma Biological process cellular response to organonitrogen compound steroid metabolic process cellular response to glucose starvation sterol biosynthetic process lipid biosynthetic process regulation of transcription, DNA-templated response to hypoxia cellular response to ethanol glucose homeostasis positive regulation of skeletal muscle tissue development protein heterooligomerization rhythmic process phosphorylation lipid metabolism regulation of vesicle-mediated transport negative regulation of apoptotic process cholesterol metabolic process Wnt signaling pathway response to activity fatty acid metabolic process transcription, DNA-templated cellular response to prostaglandin E stimulus autophagy protein phosphorylation negative regulation of TOR signaling cold acclimation positive regulation of gene expression response to camptothecin fatty acid biosynthetic process fatty acid homeostasis regulation of circadian rhythm fatty acid oxidation regulation of peptidyl-serine phosphorylation cellular response to nutrient levels positive regulation of cell population proliferation negative regulation of glucosylceramide biosynthetic process glucose metabolic process positive regulation of cholesterol biosynthetic process response to caffeine cholesterol biosynthetic process negative regulation of lipid catabolic process response to gamma radiation macroautophagy response to UV positive regulation of glycolytic process response to hydrogen peroxide cellular response to hypoxia signal transduction cellular response to hydrogen peroxide steroid biosynthetic process regulation of signal transduction by p53 class mediator positive regulation of autophagy CAMKK-AMPK signaling cascade regulation of macroautophagy chromatin organization positive regulation of mitochondrial transcription positive regulation of protein targeting to mitochondrion negative regulation of gene expression cellular response to oxidative stress intracellular signal transduction negative regulation of insulin receptor signaling pathway motor behavior regulation of stress granule assembly neuron cellular homeostasis regulation of microtubule cytoskeleton organization cellular response to calcium ion cellular response to glucose stimulus energy homeostasis positive regulation of protein localization negative regulation of tubulin deacetylation positive regulation of peptidyl-lysine acetylation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5562 105787 Ensembl ENSG00000132356 ENSMUSG00000050697 UniProt Q13131 Q5EG47 RefSeq (mRNA) NM_006251 NM_206907 NM_001013367 NM_001355640 RefSeq (protein) NP_006242 NP_996790 NP_001341957 NP_001341958 NP_001341963 NP_001341964 NP_001341965 NP_001341966 NP_001013385 NP_001342569 Location (UCSC) Chr 5: 40.76 – 40.8 Mb Chr 15: 5.17 – 5.21 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. ^{[5] [6]}

The protein encoded by this gene belongs to the serine/threonine protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. ^[6] A recent study proposes a role in the metastatic cascade and phenotype determination of pancreatic cancer. ^[7]

Interactions

Protein kinase, AMP-activated, alpha 1 has been shown to interact with TSC2. ^{[8] [9]}

References

1. GRCh38: Ensembl release 89: ENSG00000132356 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000050697 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Stapleton D, Mitchelhill KI, Gao G, Widmer J, Michell BJ, Teh T, et al. (Feb 1996). "Mammalian AMP-activated protein kinase subfamily". Journal of Biological Chemistry. 271 (2): 611–614. doi: 10.1074/jbc.271.2.611 . PMID   8557660.
6. "Entrez Gene: PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit".
7. Schneider C, Hilbert J, Genevaux F, Hofer S, Krauss L, Schicktanz F, et al. (2024-06-17). "A Novel AMPK Inhibitor Sensitizes Pancreatic Cancer Cells to Ferroptosis Induction". Advanced Science. 11 (31) 2307695. Weinheim, Baden-wurttemberg, Germany. Bibcode:2024AdvSc..1107695S. doi: 10.1002/advs.202307695 . ISSN   2198-3844. PMC   11336956 . PMID   38885414.
8. Inoki K, Zhu T, Guan KL (Nov 2003). "TSC2 mediates cellular energy response to control cell growth and survival". Cell. 115 (5). United States: 577–590. doi: 10.1016/S0092-8674(03)00929-2 . ISSN   0092-8674. PMID   14651849. S2CID   18173817.
9. Shaw RJ, Bardeesy N, Manning BD, Lopez L, Kosmatka M, DePinho RA, et al. (Jul 2004). "The LKB1 tumor suppressor negatively regulates mTOR signaling". Cancer Cell. 6 (1). United States: 91–99. doi: 10.1016/j.ccr.2004.06.007 . ISSN   1535-6108. PMID   15261145.

Further reading

• Munday MR, Campbell DG, Carling D, Hardie DG (1988). "Identification by amino acid sequencing of three major regulatory phosphorylation sites on rat acetyl-CoA carboxylase". European Journal of Biochemistry. 175 (2): 331–338. doi: 10.1111/j.1432-1033.1988.tb14201.x . PMID   2900138.
• Woods A, Cheung PC, Smith FC, et al. (1996). "Characterization of AMP-activated protein kinase beta and gamma subunits. Assembly of the heterotrimeric complex in vitro". Journal of Biological Chemistry. 271 (17): 10282–10290. doi: 10.1074/jbc.271.48.30517 . PMID   8626596.
• Hawley SA, Davison M, Woods A, Davies SP, Beri RK, Carling D, et al. (1996). "Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP-activated protein kinase". Journal of Biological Chemistry. 271 (44): 27879–27887. doi: 10.1074/jbc.271.44.27879 . PMID   8910387.
• Stapleton D, Woollatt E, Mitchelhill KI, Nicholl JK, Fernandez CS, Michell BJ, et al. (1997). "AMP-activated protein kinase isoenzyme family: subunit structure and chromosomal location". FEBS Letters. 409 (3): 452–456. Bibcode:1997FEBSL.409..452S. doi:10.1016/S0014-5793(97)00569-3. PMID   9224708. S2CID   39329574.
• Velasco G, Gómez del Pulgar T, Carling D, Guzmán M (1998). "Evidence that the AMP-activated protein kinase stimulates rat liver carnitine palmitoyltransferase I by phosphorylating cytoskeletal components". FEBS Letters. 439 (3): 317–320. Bibcode:1998FEBSL.439..317V. doi: 10.1016/S0014-5793(98)01400-8 . PMID   9845345. S2CID   29493620.
• Crute BE, Seefeld K, Gamble J, Kemp BE, Witters LA (1999). "Functional domains of the alpha1 catalytic subunit of the AMP-activated protein kinase". Journal of Biological Chemistry. 273 (52): 35347–35354. doi: 10.1074/jbc.273.52.35347 . PMID   9857077.
• Xavier G, Leclerc I, Salt IP, Doiron B, Hardie DG, Kahn A, et al. (2000). "Role of AMP-activated protein kinase in the regulation by glucose of islet beta cell gene expression". Proceedings of the National Academy of Sciences of the United States of America. 97 (8): 4023–4028. Bibcode:2000PNAS...97.4023D. doi: 10.1073/pnas.97.8.4023 . PMC   18135 . PMID   10760274.
• Hallows KR, Raghuram V, Kemp BE, Witters LA, Foskett JK (2000). "Inhibition of cystic fibrosis transmembrane conductance regulator by novel interaction with the metabolic sensor AMP-activated protein kinase". The Journal of Clinical Investigation. 105 (12): 1711–1721. doi:10.1172/JCI9622. PMC   378514 . PMID   10862786.
• Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, et al. (2001). "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells". Genome Research. 10 (10): 1546–1560. doi:10.1101/gr.140200. PMC   310934 . PMID   11042152.
• Chen ZP, McConell GK, Michell BJ, Snow RJ, Canny BJ, Kemp BE (2000). "AMPK signaling in contracting human skeletal muscle: acetyl-CoA carboxylase and NO synthase phosphorylation". American Journal of Physiology. Endocrinology and Metabolism. 279 (5): E1202–6. doi:10.1152/ajpendo.2000.279.5.E1202. PMID   11052978. S2CID   13387968.
• Blázquez C, Geelen MJ, Velasco G, Guzmán M (2001). "The AMP-activated protein kinase prevents ceramide synthesis de novo and apoptosis in astrocytes". FEBS Letters. 489 (2–3): 149–153. Bibcode:2001FEBSL.489..149B. doi: 10.1016/S0014-5793(01)02089-0 . PMID   11165240. S2CID   35584617.
• Diggle TA, Subkhankulova T, Lilley KS, Shikotra N, Willis AE, Redpath NT (2001). "Phosphorylation of elongation factor-2 kinase on serine 499 by cAMP-dependent protein kinase induces Ca2+/calmodulin-independent activity". The Biochemical Journal. 353 (Pt 3): 621–626. doi:10.1042/0264-6021:3530621. PMC   1221608 . PMID   11171059.
• Wang X, Li W, Williams M, Terada N, Alessi DR, Proud CG (2001). "Regulation of elongation factor 2 kinase by p90(RSK1) and p70 S6 kinase". The EMBO Journal. 20 (16): 4370–4379. doi:10.1093/emboj/20.16.4370. PMC   125559 . PMID   11500364.
• Xi X, Han J, Zhang JZ (2001). "Stimulation of glucose transport by AMP-activated protein kinase via activation of p38 mitogen-activated protein kinase". Journal of Biological Chemistry. 276 (44): 41029–41034. doi: 10.1074/jbc.M102824200 . PMID   11546797.
• Fryer LG, Foufelle F, Barnes K, Baldwin SA, Woods A, Carling D (2002). "Characterization of the role of the AMP-activated protein kinase in the stimulation of glucose transport in skeletal muscle cells". The Biochemical Journal. 363 (Pt 1): 167–174. doi:10.1042/0264-6021:3630167. PMC   1222463 . PMID   11903059.
• Yang CS, Weiner H (2002). "Yeast two-hybrid screening identifies binding partners of human Tom34 that have ATPase activity and form a complex with Tom34 in the cytosol". Archives of Biochemistry and Biophysics. 400 (1): 105–110. doi:10.1006/abbi.2002.2778. PMID   11913976.
• Bolster DR, Crozier SJ, Kimball SR, Jefferson LS (2002). "AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through down-regulated mammalian target of rapamycin (mTOR) signaling". Journal of Biological Chemistry. 277 (27): 23977–23980. doi: 10.1074/jbc.C200171200 . PMID   11997383.
• Esumi H, Izuishi K, Kato K, Hashimoto K, Kurashima Y, Kishimoto A, et al. (2002). "Hypoxia and nitric oxide treatment confer tolerance to glucose starvation in a 5'-AMP-activated protein kinase-dependent manner". Journal of Biological Chemistry. 277 (36): 32791–32798. doi: 10.1074/jbc.M112270200 . PMID   12091379.
• Horman S, Browne G, Krause U, Patel J, Vertommen D, Bertrand L, et al. (2003). "Activation of AMP-activated protein kinase leads to the phosphorylation of elongation factor 2 and an inhibition of protein synthesis". Current Biology. 12 (16): 1419–1423. doi: 10.1016/S0960-9822(02)01077-1 . PMID   12194824. S2CID   9865400.

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human 5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1)