PRKAB2

PRKAB2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2F15, 2V8Q, 2V92, 2V9J, 2Y8L, 2Y8Q, 2YA3, 4CFH, 4EAI, 4EAJ, 4RER, 4REW
Identifiers
Aliases	PRKAB2 , protein kinase AMP-activated non-catalytic subunit beta 2
External IDs	OMIM: 602741 MGI: 1336185 HomoloGene: 38046 GeneCards: PRKAB2
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1q21.1 Start 147,155,106 bp [1] End 147,172,550 bp [1]
Gene location (Mouse) Chr. Chromosome 3 (mouse) [2] Band 3|3 F2.2 Start 97,565,509 bp [2] End 97,581,128 bp [2]
RNA expression pattern Bgee Top expressed in biceps brachii vastus lateralis muscle jejunal mucosa muscle of leg Body of pancreas gastrocnemius muscle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function AMP-activated protein kinase activity GO:0001948 protein binding identical protein binding protein kinase binding Cellular component cytosol nucleoplasm nucleotide-activated protein kinase complex nucleus cytoplasm Biological process lipid metabolism regulation of fatty acid biosynthetic process fatty acid metabolic process fatty acid biosynthetic process macroautophagy protein phosphorylation signal transduction GO:0048552 regulation of catalytic activity positive regulation of cold-induced thermogenesis carnitine shuttle regulation of macroautophagy regulation of signal transduction by p53 class mediator Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5565	108097
Ensembl	ENSG00000131791	ENSMUSG00000038205
UniProt	O43741	Q6PAM0
RefSeq (mRNA)	NM_005399	NM_182997
RefSeq (protein)	NP_005390	NP_892042
Location (UCSC)	Chr 1: 147.16 – 147.17 Mb	Chr 3: 97.57 – 97.58 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

5'-AMP-activated protein kinase subunit beta-2 is an enzyme that in humans is encoded by the PRKAB2 gene. ^{[5] [6]}

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. ^[6]

Related gene problems

• 1q21.1 deletion syndrome
• 1q21.1 duplication syndrome

Interactions

PRKAB2 has been shown to interact with PRKAG2 ^[7] and PRKAG1. ^[7]

Research on the genes CHD1L and PRKAB2 within lymphoblast cells ^[8] lead to the conclusion that anomalies appear with the 1q21.1 deletion syndrome:

• CHD1L is an enzyme which is involved in untangling the chromatids and the DNA repair system. With 1q21.1 deletion syndrome a disturbance occurs, which leads to increased DNA breaks. The role of CHD1L is similar to that of helicase with the Werner syndrome
• PRKAB2 is involved in maintaining the energy level of cells. With 1q21.1-deletion syndrome this function was attenuated.

References

1. GRCh38: Ensembl release 89: ENSG00000131791 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000038205 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Stapleton D, Mitchelhill KI, Gao G, Widmer J, Michell BJ, Teh T, House CM, Fernandez CS, Cox T, Witters LA, Kemp BE (February 1996). "Mammalian AMP-activated protein kinase subfamily". J Biol Chem. 271 (2): 611–4. doi: 10.1074/jbc.271.2.611 . PMID   8557660.
6. "Entrez Gene: PRKAB2 protein kinase, AMP-activated, beta 2 non-catalytic subunit".
7. Cheung, P C; Salt I P; Davies S P; Hardie D G; Carling D (March 2000). "Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding". Biochem. J. ENGLAND. 346 (3): 659–69. doi:10.1042/0264-6021:3460659. ISSN   0264-6021. PMC   1220898 . PMID   10698692.
8. Harvard C, Strong E, Mercier E, Colnaghi R, Alcantara D, Chow E, Martell S, Tyson C, Hrynchak M, McGillivray B, Hamilton S, Marles S, Mhanni A, Dawson AJ, Pavlidis P, Qiao Y, Holden JJ, Lewis SM, O'Driscoll M, Rajcan-Separovic E (2011). "Understanding the impact of 1q21.1 copy number variant". Orphanet J Rare Dis. 6: 54. doi:10.1186/1750-1172-6-54. PMC   3180300 . PMID   21824431.

Further reading

• Gao G, Fernandez CS, Stapleton D, et al. (1996). "Non-catalytic beta- and gamma-subunit isoforms of the 5'-AMP-activated protein kinase". J. Biol. Chem. 271 (15): 8675–81. doi: 10.1074/jbc.271.15.8675 . PMID   8621499.
• Woods A, Cheung PC, Smith FC, et al. (1996). "Characterization of AMP-activated protein kinase beta and gamma subunits. Assembly of the heterotrimeric complex in vitro". J. Biol. Chem. 271 (17): 10282–90. doi: 10.1074/jbc.271.48.30517 . PMID   8626596.
• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi: 10.1101/gr.6.9.791 . PMID   8889548.
• Stapleton D, Woollatt E, Mitchelhill KI, et al. (1997). "AMP-activated protein kinase isoenzyme family: subunit structure and chromosomal location". FEBS Lett. 409 (3): 452–6. doi:10.1016/S0014-5793(97)00569-3. PMID   9224708. S2CID   39329574.
• Thornton C, Snowden MA, Carling D (1998). "Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle". J. Biol. Chem. 273 (20): 12443–50. doi: 10.1074/jbc.273.20.12443 . PMID   9575201.
• Cheung PC, Salt IP, Davies SP, et al. (2000). "Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding". Biochem. J. 346 (3): 659–69. doi:10.1042/0264-6021:3460659. PMC   1220898 . PMID   10698692.
• Xu XR, Huang J, Xu ZG, et al. (2002). "Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver". Proc. Natl. Acad. Sci. U.S.A. 98 (26): 15089–94. Bibcode:2001PNAS...9815089X. doi: 10.1073/pnas.241522398 . PMC   64988 . PMID   11752456.
• Park SH, Paulsen SR, Gammon SR, et al. (2003). "Effects of thyroid state on AMP-activated protein kinase and acetyl-CoA carboxylase expression in muscle". J. Appl. Physiol. 93 (6): 2081–8. doi:10.1152/japplphysiol.00504.2002. PMID   12433937.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Prochazka M, Farook VS, Ossowski V, et al. (2003). "Variant screening of PRKAB2, a type 2 diabetes mellitus susceptibility candidate gene on 1q in Pima Indians". Mol. Cell. Probes. 16 (6): 421–7. doi:10.1006/mcpr.2002.0439. PMID   12490143.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Minokoshi Y, Alquier T, Furukawa N, et al. (2004). "AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus". Nature. 428 (6982): 569–74. Bibcode:2004Natur.428..569M. doi:10.1038/nature02440. PMID   15058305. S2CID   4302317.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC   1356129 . PMID   16344560.
• Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315–21. Bibcode:2006Natur.441..315G. doi: 10.1038/nature04727 . PMID   16710414.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC   1847948 . PMID   17353931.