Voltage-gated proton channel

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Typical voltage-gated ion channel in the closed state (left) and open state (right). Voltage-gated Ion Channel.png
Typical voltage-gated ion channel in the closed state (left) and open state (right).
Classical voltage-gated cation channel a-subunit. S1-S4 transmembrane helices form the voltage-sensing domain and S5-S6 form the pore domain. Voltage-gated ion channel simplified structure.svg
Classical voltage-gated cation channel α-subunit. S1–S4 transmembrane helices form the voltage-sensing domain and S5–S6 form the pore domain.

Voltage-gated proton channels are ion channels that conduct protons in a voltage- and pH-dependent manner. Compared to most voltage-gated cation channels, which contain a separate pore domain, these channels conduct protons through the voltage-sensing domain itself and open only when the electrochemical gradient favors outward proton flux. [1] [2]

Contents

In vertebrates the best-characterized voltage-gated proton channel is Hv1, encoded by the HVCN1 gene. Hv1 contributes to pH homeostasis and charge compensation in several cell types, including phagocytes, B cells, sperm, microglia, and osteoclasts. [1] [3] [4] [5] Hv-like channels are present in a wide range of eukaryotes, including many animals, protists, and plants, but have not been identified in bacteria or archaea. [6] [7]

Structure

Voltage-gated proton channels are membrane proteins built from a voltage-sensing domain with four transmembrane helices, S1 to S4. They lack the S5–S6 pore domain that forms the central ion conduction pathway in most voltage-gated potassium and sodium channels, so proton permeation occurs within the voltage-sensing domain itself. [1] [2]

Topology of an Hv-type voltage-gated proton channel subunit with the fewer four transmembrane helices (S1-S4). Hv1 simplified structure.svg
Topology of an Hv-type voltage-gated proton channel subunit with the fewer four transmembrane helices (S1–S4).

The human Hv1 protein is a dimer of two identical subunits. Each subunit contains its own voltage-sensing domain and forms an independent proton conduction pathway, so a dimer has two functional pores. [8] [2] The C-terminal cytosolic regions of the subunits form a coiled-coil, which stabilizes the dimer and supports cooperative gating between the two subunits. [2] [9]

Structural work and simulations indicate that proton permeation occurs along a chain of water molecules and titratable side chains within the voltage-sensing domain. [10] [11] [1] [12] A conserved aspartate in S1 (Asp112 in human Hv1) acts as the main selectivity site for protons. Neutralization or charge reversal at this position disrupts proton selectivity and allows other small cations, including guanidinium, to pass through. [9] [10] [13] The hydrogen-bond network around this aspartate and nearby basic residues in S4 is important for maintaining proton selectivity. [9] [12]

Voltage and pH-dependent gating

Idealized conductance-voltage (G/Gmax) curves for Hv1 at different transmembrane pH gradients (DpH). The activation curve shifts by about 40 mV per pH unit, so the channel opens only under conditions that favor outward proton flux. Image generated with python.png
Idealized conductance–voltage (G/Gmax) curves for Hv1 at different transmembrane pH gradients (ΔpH). The activation curve shifts by about 40 mV per pH unit, so the channel opens only under conditions that favor outward proton flux.

Voltage-gated proton channels are activated by membrane depolarization, but their gating is strongly influenced by the transmembrane pH gradient. The voltage-dependence of Hv1 activation shifts by roughly 40 millivolts for each unit change in the pH difference across the membrane, so the activation curve follows the reversal potential for protons. [14] [1] [15] Under physiological conditions, these channels open only when the electrochemical gradient drives protons out of the cell, and macroscopic currents are almost exclusively outward.

Because of this coupling to the pH gradient, Hv1 tends to oppose sustained depolarization during proton efflux and to support cytosolic pH regulation, rather than generating large, fast depolarizing currents. [1] [16] [17] The estimated flux through a single Hv1 channel is on the order of 100,000 protons per second under typical conditions, which is small compared with many other ion channels but is sufficient for long-lasting metabolic loads. [18] [19]

The molecular basis of the pH dependence involves protonation of residues that are accessible from the intracellular and extracellular solutions. Changes in pH shift the equilibrium of voltage-sensor movements in S4 and thereby shift the voltage range over which the channel opens. [15] [20] [9] Proton-conducting "gating-pore" currents that appear in some mutant voltage-gated sodium channels are often used as an analogy for the conduction pathway in Hv1. [1] [15]

Zn2+ cation binding to Hv1 in the extracellular side by S1 and S3 subunits coordinated by histidines (Right) vs. a Hv1 without Zn2+ cation (Left). Hv1 with and without zinc.png
Zn²⁺ cation binding to Hv1 in the extracellular side by S1 and S3 subunits coordinated by histidines (Right) vs. a Hv1 without Zn²⁺ cation (Left).

Inhibition

External zinc ions are potent inhibitors of Hv1. Micromolar concentrations of zinc applied to the extracellular side shift activation to more positive voltages and reduce current amplitude. [21] [1] Histidines in the extracellular parts of S1 and S3, along with residues at the dimer interface, contribute to high-affinity zinc binding. In electrophysiological recordings, reversible zinc block is a common criterion for identifying Hv currents. [21] [9]

Several small-molecule Hv1 inhibitors have been developed and tested in cells and in animal models. [22] [23] [24] These compounds are used as experimental tools and are being evaluated as potential anti-inflammatory or anticancer agents, but they are not in clinical use as approved drugs.

Distribution and evolution

Comparative genomics indicates that Hv-like channels are widespread in eukaryotes but have not been detected in bacteria or archaea. [6] Vertebrates typically possess a single Hv gene, HVCN1, while several invertebrate and unicellular lineages have multiple paralogues. [25] [6] Many commonly used invertebrate models, including Drosophila melanogaster and Caenorhabditis elegans, do not appear to have Hv genes. [6]

In some molluscs and other marine invertebrates, the Hv gene family is expanded, with several Hv channels expressed within a single species. [6] [25] Hv channels have also been identified in dinoflagellates, where they support large proton currents in marine protists. [26] [6]

Hv1 orthologues are present in land plants. In Arabidopsis thaliana, Hv1 contributes to pH regulation in pollen and other cells. In angiosperms, heterologous expression studies suggest that Hv channels often require prior mechanical perturbation of the membrane for robust voltage-dependent activation, whereas Hv channels from non-flowering plants tested so far do not show this requirement. [7]

Physiological roles

Phagocytes and innate immunity

Hv1 is expressed in phagocytes such as neutrophils, eosinophils, and macrophages. During the respiratory burst, the NADPH oxidase complex transfers electrons across the plasma or phagosomal membrane to generate superoxide and other reactive oxygen species. [27] [28] [3] This electron flux depolarizes the membrane and acidifies the cytosol.

Hv1 provides charge compensation and proton extrusion during the respiratory burst. In cells lacking Hv1 or in the presence of zinc, superoxide production is markedly reduced, whereas phagocytosis itself is largely preserved. [29] [13] [22] [30] By exporting protons, Hv1 helps maintain cytosolic pH within a workable range for enzyme activity and prevents excessive depolarization that would otherwise slow or stop the oxidase.

Hv1-dependent functions in phagocytes have been described in mammals and in zebrafish neutrophils, which indicates that this role is conserved across vertebrates. [30] [5]

B cells and other immune cells

Hv1 is expressed in B lymphocytes, where it supports antigen receptor signaling. In mouse models, loss of HVCN1 reduces B cell receptor–dependent reactive oxygen species production and attenuates downstream signaling events. [4] Hv1 has also been implicated in T cell lines and other leukocytes, where pharmacological inhibition can shift intracellular pH and promote apoptosis in some malignant cells. [23] [31]

Nervous system and microglia

Hv1 is present in microglia and other cells of the nervous system. Microglial Hv1 contributes to reactive oxygen species production during inflammatory activation and has been implicated in neuronal injury after ischemia and spinal cord trauma. [19] [32] [5] In mouse models, genetic deletion or pharmacological inhibition of Hv1 reduces microglial reactive oxygen species production and can lessen tissue damage in several central nervous system injury paradigms. [19] [32] [33]

Sperm

Human Hv1 is localized along the principal piece of the sperm flagellum together with the calcium channel CatSper. [34] When sperm move from the acidic environment of semen and the epididymis into the more alkaline female reproductive tract, Hv1 opens in response to the change in pH and membrane potential. Proton efflux through Hv1 raises intracellular pH, which in turn facilitates CatSper activation and calcium influx. [34]

Block of Hv1 in human sperm reduces intracellular alkalinization, decreases calcium entry through CatSper, and impairs hyperactivated motility and the acrosome reaction in vitro. [34] These findings support a role for Hv1 in human fertilization.

Osteoclasts and other tissues

Hv1 is expressed in osteoclasts, where it contributes to the export of protons into the resorption lacuna together with vacuolar proton pumps. [35] [36] Hv1 carries a smaller fraction of the total proton flux than the pumps but can facilitate acidification under some conditions [35]

Hv1 currents have been recorded or inferred in several other cell types that experience sustained proton loads, including airway epithelial cells, certain neurons, and some tumor cells. In these settings, Hv1 generally supports pH homeostasis during prolonged metabolic activity. [1] [5] [24]

Cancer and pathophysiology

Hv1 is upregulated in several human cancers. In breast cancer, Hv1 expression is elevated in highly metastatic cell lines and in metastatic tumor tissues compared with non-metastatic samples. Reducing Hv1 expression in these models decreases tumor growth, extracellular acidification, and metastasis. [37] [31] [24] In colorectal cancer, Hv1 is overexpressed in primary tumors and is associated with poorer prognosis. [38] [24] Hv1 has also been studied as a potential drug target in conditions where sustained proton efflux, acidic microenvironments, and reactive oxygen species signaling are involved, including cancer and inflammatory conditions [1] [24] [37] [38]

Proposed mechanisms include support of glycolytic metabolism by maintaining intracellular pH, enhancement of extracellular acidification, and modulation of reactive oxygen species signaling that affects migration and invasion. [24] [1] Hv1 is therefore being investigated as a potential biomarker and therapeutic target in oncology, although no Hv1-targeted drugs have yet progressed to clinical approval.

Outside of cancer, altered Hv1 activity has been linked to inflammatory and ischemic conditions, particularly in the central nervous system, where Hv1-dependent microglial reactive oxygen species contribute to neuronal injury in several experimental models. [19] [32] [33]

References

  1. 1 2 3 4 5 6 7 8 9 10 11 DeCoursey, T E (2013). "Voltage-gated proton channels: molecular biology, physiology, and pathophysiology of the H(V) family". Physiological Reviews. 93 (2): 599–652. doi:10.1152/physrev.00011.2012. PMC   3677779 . PMID   23589829.
  2. 1 2 3 4 Takeshita, K; Sakata, S; Yamashita, E; Fujiwara, Y (2014). "X-ray crystal structure of the voltage-gated proton channel Hv1". Nature Structural & Molecular Biology. 21 (4): 352–357. doi:10.1038/nsmb.2783. PMID   24584463.
  3. 1 2 Murphy, R; DeCoursey, T E (2006). "Charge compensation during the phagocyte respiratory burst". Biochimica et Biophysica Acta – Bioenergetics. 1757 (8): 996–1011. doi:10.1016/j.bbabio.2006.01.005. PMID   16483534.
  4. 1 2 Capasso, M; Bhamrah, M K; Henley, T; Boyd, R S (2010). "HVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species". Nature Immunology. 11 (3): 265–272. doi:10.1038/ni.1843.
  5. 1 2 3 4 Shen, Y; Luo, Y; Liao, P; Zuo, Y; Jiang, R (2023). "Role of the voltage-gated proton channel Hv1 in nervous systems". Neuroscience Bulletin. 39 (7): 1157–1172. doi:10.1007/s12264-023-01053-6. PMID   37029856.
  6. 1 2 3 4 5 6 Chaves, G; Verma, N; Liu, Y; Tombola, F (2023). "Voltage-gated proton channels in the tree of life". Biomolecules. 13 (7): 1035. doi: 10.3390/biom13071035 .
  7. 1 2 Zhao, C; Huang, X; Jacobsen, C S; Li, H; Guo, Y (2023). "Mechanically-primed voltage-gated proton channels from angiosperm plants". Nature Communications. 14 (1): 7515. Bibcode:2023NatCo..14.7515Z. doi:10.1038/s41467-023-43280-5. PMC   10657467 . PMID   37980353.
  8. ↑ Tombola, F; Ulbrich, M H; Isacoff, E Y (2008). "The voltage-gated proton channel Hv1 has two pores, each controlled by one voltage sensor". Neuron. 58 (4): 546–556. doi:10.1016/j.neuron.2008.03.026. PMC   2430592 . PMID   18498736.
  9. 1 2 3 4 5 DeCoursey, T E; Morgan, D; Musset, B; Cherny, V V (2016). "Insights into the structure and function of HV1 from a meta-analysis of mutation studies". Journal of General Physiology. 148 (2): 97–118. doi:10.1085/jgp.201611619. PMC   4969798 . PMID   27481712.
  10. 1 2 Ramsey, I S; Moran, M M; Chung, S; Clapham, D E (2010). "An aqueous H⁺ permeation pathway in the voltage-gated proton channel Hv1". Nature Structural & Molecular Biology. 17 (7): 869–875. doi:10.1038/nsmb.1826. PMID   20543828.
  11. ↑ Schow, E V; Wood, M L; Petersen, K J; Thomas, J L (2012). "Water wires in atomistic models of the Hv1 proton channel". Biochimica et Biophysica Acta – Biomembranes. 1818 (2): 286–293. doi:10.1016/j.bbamem.2011.07.045. PMC   3245885 . PMID   21843503.
  12. 1 2 Liu, Y; Verma, N; Chaves, G; Tombola, F (2024). "Quantitative insights into the mechanism of proton selectivity in hHv1". Proceedings of the National Academy of Sciences of the United States of America. 121 (38) e2407479121. doi:10.1073/pnas.2407479121. PMC   11420211 . PMID   39259593.
  13. 1 2 Musset, B; Cherny, V V; Saa, T; Sers, A; DeCoursey, T E (2011). "Hv1 proton channels in neutrophils facilitate ROS production but are dispensable for phagocytosis". Journal of Biological Chemistry. 286 (15): 13096–13106.
  14. ↑ Cherny, V V; Markin, V S; DeCoursey, T E (1995). "The voltage-activated hydrogen ion conductance in rat alveolar epithelial cells is determined by the pH gradient". Journal of General Physiology. 105 (6): 861–896. doi:10.1085/jgp.105.6.861. PMC   2216954 . PMID   7561747.
  15. 1 2 3 DeCoursey, T E (2018). "Voltage and pH sensing by the voltage-gated proton channel, HV1". Journal of the Royal Society Interface. 15 (140) 20180108. doi:10.1098/rsif.2018.0108. PMC   5938591 . PMID   29643227.
  16. ↑ Musset, B; DeCoursey, T E (2012). "Biophysical properties of the voltage-gated proton channel HV1". Wiley Interdisciplinary Reviews: Membrane Transport and Signaling. 1 (5): 605–620. doi:10.1002/wmts.55. PMC   3462886 . PMID   23050239.
  17. ↑ De-la-Rosa, V; Suarez-Delgado, I; Ramirez, A (2016). "Currents through Hv1 channels deplete protons in their microenvironment". Journal of General Physiology. 147 (2): 127–145. doi:10.1085/jgp.201511496. PMID   26809792.
  18. ↑ DeCoursey, T E; Hosler, J (2014). "Philosophy of voltage-gated proton channels". Journal of the Royal Society Interface. 11 (92) 20130799. doi:10.1098/rsif.2013.0799. PMC   3899857 . PMID   24352668.
  19. 1 2 3 4 Li, X (2020). "Deficiency of the microglial Hv1 proton channel attenuates neuronal pyroptosis and inhibits inflammatory reaction after spinal cord injury". Journal of Neuroinflammation. 17 263. doi: 10.1186/s12974-020-01942-x . PMC   7487532 . PMID   32891159.
  20. ↑ Han, S; Gonzalez-Perrett, S; Cao, Y; Tombola, F (2022). "Structural dynamics determine voltage and pH gating in human Hv1". eLife. 11 e73093. doi: 10.7554/eLife.73093 . PMC   8926398 . PMID   35244539.
  21. 1 2 Hong, L; Pathak, M M; Kim, I H; Ta, D; Tombola, F (2013). "Voltage-sensing domain of voltage-gated proton channel Hv1 shares mechanism of block with pore domains". Neuron. 77 (2): 274–287. doi:10.1016/j.neuron.2012.11.013. PMC   3559007 . PMID   23352164.
  22. 1 2 Ramsey, I S; Moran, M M; Chung, S; Clapham, D E (2010). "An aqueous H⁺ permeation pathway in the voltage-gated proton channel Hv1". Nature Structural & Molecular Biology. 17 (7): 869–875. doi:10.1038/nsmb.1826. PMID   20543828.
  23. 1 2 Asuaje, A; Smaldini, P L; Martin, P; Enyedi, P (2016). "Inhibition of the voltage-gated H⁺ channel (HVCN1) induces acidification of leukemic Jurkat T cells and promotes apoptosis". Pflügers Archiv – European Journal of Physiology. 468 (5): 901–914.
  24. 1 2 3 4 5 6 Alvear-Arias, J J; Gomez, H; Tombola, F (2023). "Role of voltage-gated proton channel (Hv1) in cancer biology". Frontiers in Pharmacology. 14 1175702. doi: 10.3389/fphar.2023.1175702 . PMC   10157179 . PMID   37153807.
  25. 1 2 Chaves, G; Hernández-Carballo, C Y; Carrillo, E; Tombola, F (2016). "Identification of an Hv1 voltage-gated proton channel in insects". FEBS Journal. 283 (15): 2657–2675. doi:10.1111/febs.13680. PMID   26866814.
  26. ↑ Zhou, Q; Chen, J; Enoch, S; Hossain, M I; Okamura, Y (2015). "Voltage-gated proton channel in a dinoflagellate". Proceedings of the National Academy of Sciences of the United States of America. 112 (11): 3627–3632.
  27. ↑ Babior, B M (1999). "NADPH oxidase: an update". Blood. 93 (5): 1464–1476. doi:10.1182/blood.V93.5.1464. PMID   10029572.
  28. ↑ Henderson, L M; Chappell, J B; Jones, O T (1987). "The superoxide-generating NADPH oxidase of human neutrophils is electrogenic and associated with an H⁺ channel". Biochemical Journal. 246 (2): 325–329. doi:10.1042/bj2460325. PMC   1148280 . PMID   2825632.
  29. ↑ El Chemaly, A; Okochi, Y; Sasaki, M; Arnaudeau, S; Okamura, Y; Demaurex, N (2010). "HV1 proton channels are required for high-level NADPH oxidase–dependent superoxide production during the phagocyte respiratory burst". Proceedings of the National Academy of Sciences of the United States of America. 107 (2): 838–843.
  30. 1 2 Ratanayotha, A; Kawai, T; Higashijima, S I; Okamura, Y (2017). "Molecular and functional characterization of the voltage-gated proton channel in zebrafish neutrophils". Physiological Reports. 5 (15) e13345. doi:10.14814/phy2.13345. PMC   5555884 . PMID   28774948.
  31. 1 2 Bare, D J; Gomez, H; McNally, B; Tombola, F (2020). "Expression and function of voltage-gated proton channels (Hv1) in breast cancer cells". PLOS ONE. 15 (3) e0227522. doi: 10.1371/journal.pone.0227522 . PMC   7202653 . PMID   32374759.
  32. 1 2 3 Peng, J; Wang, Y; Zhang, X (2021). "The voltage-gated proton channel Hv1 promotes microglia-mediated neuronal injury". Glia. 69 (7): 1630–1648.
  33. 1 2 Tang, Y; Yang, X; Zhang, Y (2024). "The emerging role of microglial Hv1 as a target for immunomodulation in myelin repair". Aging and Disease. 15: 1176–1203.
  34. 1 2 3 Lishko, P V; Botchkina, I L; Kirichok, Y (2011). "Progesterone activates the principal Ca²⁺ channel of human sperm". Nature. 471 (7338): 387–391. doi:10.1038/nature09767. PMID   21412339.
  35. 1 2 Marcoline, F V; Irazabal, I; Li, Y (2016). "A mathematical model of osteoclast acidification during bone resorption". Bone. 93: 167–180. doi:10.1016/j.bone.2016.09.007. PMC   5077641 . PMID   27650914.
  36. ↑ Di Pompo, G; Lombardi, G; Jacques, C (2021). "Acid microenvironment in bone sarcomas". Cancers. 13 (15): 3848. doi: 10.3390/cancers13153848 . PMC   8345667 . PMID   34359749.
  37. 1 2 Wang, Y; Li, S J; Pan, J; Che, Y (2011). "Specific expression of the human voltage-gated proton channel Hv1 in highly metastatic breast cancer cells promotes tumor progression and metastasis". Biochemical and Biophysical Research Communications. 412 (2): 353–359. Bibcode:2011BBRC..412..353W. doi:10.1016/j.bbrc.2011.07.102. PMID   21821008.
  38. 1 2 Wang, Y; Wu, X; Li, Q; Zhang, S; Li, S J (2013). "Human voltage-gated proton channel Hv1: a new potential biomarker for diagnosis and prognosis of colorectal cancer". PLOS ONE. 8 (8) e70550. Bibcode:2013PLoSO...870550W. doi: 10.1371/journal.pone.0070550 . PMC   3734282 . PMID   23940591.
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