EIF4B

EIF4B
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1WI8, 2J76
Identifiers
Aliases	EIF4B , EIF-4B, PRO1843, eukaryotic translation initiation factor 4B
External IDs	OMIM: 603928 MGI: 95304 HomoloGene: 83162 GeneCards: EIF4B
Gene location (Human) Chr. Chromosome 12 (human) [1] Band 12q13.13 Start 53,006,282 bp [1] End 53,042,215 bp [1]
Gene location (Mouse) Chr. Chromosome 15 (mouse) [2] Band 15|15 F2 Start 101,982,208 bp [2] End 102,005,608 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Achilles tendon ganglionic eminence islet of Langerhans stromal cell of endometrium body of pancreas monocyte canal of the cervix gastric mucosa gastrocnemius muscle gallbladder Top expressed in lacrimal gland ankle somite left lung lobe vas deferens parotid gland maxillary prominence Paneth cell uterus molar More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding nucleic acid binding ribosomal small subunit binding helicase activity RNA strand-exchange activity RNA strand annealing activity RNA binding translation initiation factor activity Cellular component cytosol eukaryotic translation initiation factor 4F complex polysome Biological process nuclear-transcribed mRNA poly(A) tail shortening regulation of translational initiation formation of translation preinitiation complex eukaryotic translation initiation factor 4F complex assembly protein biosynthesis translational initiation cytoplasmic translation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1975 75705 Ensembl ENSG00000063046 ENSMUSG00000058655 UniProt P23588 Q8BGD9 RefSeq (mRNA) NM_001300821 NM_001417 NM_018507 NM_001330654 NM_145625 RefSeq (protein) NP_001287750 NP_001317583 NP_001408 NP_663600 Location (UCSC) Chr 12: 53.01 – 53.04 Mb Chr 15: 101.98 – 102.01 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Eukaryotic translation initiation factor 4B is a protein that in humans is encoded by the EIF4B gene. ^[5]

Interactions

eIF4B has been shown to interact with and stimulate the activity of eIF4A and bind to the eIF3 complex through the eIF3A subunit. ^[6] This interaction results in the recruitment of the eukaryotic small ribosomal subunit (40S) to the mRNA which will in turn set the stage for the later steps leading to elongation.

See also

• Eukaryotic translation
• eIF4F

References

1. GRCh38: Ensembl release 89: ENSG00000063046 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000058655 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: EIF4B eukaryotic translation initiation factor 4B".
6. Merrick WC (Oct 2015). "eIF4F: a retrospective". The Journal of Biological Chemistry. 290 (40): 24091–9. doi: 10.1074/jbc.R115.675280 . PMC   4591800 . PMID   26324716.

Further reading

• van Heugten HA, Kasperaitis MA, Thomas AA, Voorma HO (1991). "Evidence that eukaryotic initiation factor (eIF) 2 is a cap-binding protein that stimulates cap recognition by eIF-4B and eIF-4F". J. Biol. Chem. 266 (11): 7279–84. doi: 10.1016/S0021-9258(20)89641-0 . PMID   2016328.
• Milburn SC, Hershey JW, Davies MV, Kelleher K, Kaufman RJ (1990). "Cloning and expression of eukaryotic initiation factor 4B cDNA: sequence determination identifies a common RNA recognition motif". EMBO J. 9 (9): 2783–90. doi:10.1002/j.1460-2075.1990.tb07466.x. PMC   551988 . PMID   2390971.
• Howe JG, Hershey JW (1984). "Translational initiation factor and ribosome association with the cytoskeletal framework fraction from HeLa cells". Cell. 37 (1): 85–93. doi: 10.1016/0092-8674(84)90303-9 . PMID   6722878.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Méthot N, Pause A, Hershey JW, Sonenberg N (1994). "The translation initiation factor eIF-4B contains an RNA-binding region that is distinct and independent from its ribonucleoprotein consensus sequence". Mol. Cell. Biol. 14 (4): 2307–16. doi:10.1128/mcb.14.4.2307. PMC   358597 . PMID   8139536.
• Naranda T, Strong WB, Menaya J, Fabbri BJ, Hershey JW (1994). "Two structural domains of initiation factor eIF-4B are involved in binding to RNA". J. Biol. Chem. 269 (20): 14465–72. doi: 10.1016/S0021-9258(17)36646-2 . PMID   8182051.
• Méthot N, Song MS, Sonenberg N (1996). "A region rich in aspartic acid, arginine, tyrosine, and glycine (DRYG) mediates eukaryotic initiation factor 4B (eIF4B) self-association and interaction with eIF3". Mol. Cell. Biol. 16 (10): 5328–34. doi:10.1128/MCB.16.10.5328. PMC   231531 . PMID   8816444.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Richter NJ, Rogers GW, Hensold JO, Merrick WC (1999). "Further biochemical and kinetic characterization of human eukaryotic initiation factor 4H". J. Biol. Chem. 274 (50): 35415–24. doi: 10.1074/jbc.274.50.35415 . PMID   10585411.
• Bushell M, Wood W, Clemens MJ, Morley SJ (2000). "Changes in integrity and association of eukaryotic protein synthesis initiation factors during apoptosis". Eur. J. Biochem. 267 (4): 1083–91. doi: 10.1046/j.1432-1327.2000.01101.x . PMID   10672017.
• Bushell M, Wood W, Carpenter G, Pain VM, Morley SJ, Clemens MJ (2001). "Disruption of the interaction of mammalian protein synthesis eukaryotic initiation factor 4B with the poly(A)-binding protein by caspase- and viral protease-mediated cleavages". J. Biol. Chem. 276 (26): 23922–8. doi: 10.1074/jbc.M100384200 . PMID   11274152.
• Fleming K, Ghuman J, Yuan X, Simpson P, Szendröi A, Matthews S, Curry S (2003). "Solution structure and RNA interactions of the RNA recognition motif from eukaryotic translation initiation factor 4B". Biochemistry. 42 (30): 8966–75. doi:10.1021/bi034506g. PMID   12885229.
• Raught B, Peiretti F, Gingras AC, Livingstone M, Shahbazian D, Mayeur GL, Polakiewicz RD, Sonenberg N, Hershey JW (2004). "Phosphorylation of eucaryotic translation initiation factor 4B Ser422 is modulated by S6 kinases". EMBO J. 23 (8): 1761–9. doi:10.1038/sj.emboj.7600193. PMC   394247 . PMID   15071500.
• Doepker RC, Hsu WL, Saffran HA, Smiley JR (2004). "Herpes simplex virus virion host shutoff protein is stimulated by translation initiation factors eIF4B and eIF4H". J. Virol. 78 (9): 4684–99. doi:10.1128/JVI.78.9.4684-4699.2004. PMC   387725 . PMID   15078951.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi: 10.1073/pnas.0404720101 . PMC   514446 . PMID   15302935.
• Suzuki Y, Yamashita R, Shirota M, Sakakibara Y, Chiba J, Mizushima-Sugano J, Nakai K, Sugano S (2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC   515316 . PMID   15342556.
• Shahbazian D, Roux PP, Mieulet V, Cohen MS, Raught B, Taunton J, Hershey JW, Blenis J, Pende M, Sonenberg N (2006). "The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity". EMBO J. 25 (12): 2781–91. doi:10.1038/sj.emboj.7601166. PMC   1500846 . PMID   16763566.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi: 10.1016/j.cell.2006.09.026 . PMID   17081983. S2CID   7827573.