EIF5A

EIF5A
Available structures PDB Ortholog search: I3L397 PDBe I3L397 RCSB List of PDB id codes 3CPF, 5DLQ
Identifiers
Aliases	EIF5A , EIF-5A, EIF5A1, eIF5AI, eukaryotic translation initiation factor 5A, eIF-4D, FABAS
External IDs	OMIM: 600187 MGI: 106248 HomoloGene: 133803 GeneCards: EIF5A
Gene location (Human) Chr. Chromosome 17 (human) [1] Band 17p13.1 Start 7,306,999 bp [1] End 7,312,463 bp [1]
Gene location (Mouse) Chr. Chromosome 11 (mouse) [2] Band 11|11 B3 Start 69,807,540 bp [2] End 69,812,784 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in skin of abdomen stromal cell of endometrium appendix gastrocnemius muscle islet of Langerhans right adrenal gland left adrenal gland placenta body of pancreas lymph node Top expressed in somite primitive streak hand dermis maxillary prominence abdominal wall atrium hair follicle crypt of lieberkuhn of small intestine pineal gland More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein N-terminus binding ribosome binding translation elongation factor activity protein binding RNA binding U6 snRNA binding Cellular component cytoplasm cytosol endoplasmic reticulum membrane membrane nuclear pore lamellae anulatae endoplasmic reticulum extracellular exosome nucleus dendrite neuronal cell body Biological process mRNA transport protein biosynthesis positive regulation of translational elongation translational elongation mRNA export from nucleus peptidyl-lysine modification to peptidyl-hypusine nucleocytoplasmic transport protein export from nucleus positive regulation of cell population proliferation protein transport translational frameshifting positive regulation of translational termination apoptotic process positive regulation of cytosolic calcium ion concentration human ageing positive regulation of cardiac muscle cell apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of muscle cell differentiation cellular response to thyroid hormone stimulus positive regulation of reactive oxygen species metabolic process transport Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1984 276770 Ensembl ENSG00000132507 ENSG00000288145 ENSMUSG00000078812 UniProt P63241 P63242 RefSeq (mRNA) NM_001143760 NM_001143761 NM_001143762 NM_001970 NM_001370420 NM_001370421 NM_001166589 NM_001166590 NM_001166591 NM_001166592 NM_001166593 NM_001166594 NM_001166595 NM_001166596 NM_181582 RefSeq (protein) NP_001137232 NP_001137233 NP_001137234 NP_001961 NP_001357349 NP_001357350 NP_001160061 NP_001160062 NP_001160063 NP_001160064 NP_001160065 NP_001160066 NP_001160067 NP_001160068 NP_853613 Location (UCSC) Chr 17: 7.31 – 7.31 Mb Chr 11: 69.81 – 69.81 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Eukaryotic translation initiation factor 5A-1 is a protein that in humans is encoded by the EIF5A gene. ^[5]

It is the only known protein to contain the unusual amino acid hypusine [N^ε-(4-amino-2-hydroxybutyl)-lysine], which is synthesized on eIF5A at a specific lysine residue from the polyamine spermidine by two catalytic steps. ^[6]

EF-P is the bacterial homolog of eIF5A, which is modified post-translationally in a similar but distinct way. ^{[7] [8]} Both proteins are believed to catalyze peptide bond formation and help resolve ribosomal stalls, making them elongation factors despite the "initiation factor" name originally assigned. ^[9]

Clinical relevance

Germline deleterious heterozygous EIF5A variants cause Faundes-Banka syndrome. ^{[10] [11]} This rare human disorder is characterized by variable combinations of developmental delay, microcephaly, micrognathia and dysmorphic features.

References

1. ENSG00000288145 GRCh38: Ensembl release 89: ENSG00000132507, ENSG00000288145 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000078812 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Steinkasserer A, Jones T, Sheer D, Koettnitz K, Hauber J, Bevec D (February 1995). "The eukaryotic cofactor for the human immunodeficiency virus type 1 (HIV-1) rev protein, eIF-5A, maps to chromosome 17p12-p13: three eIF-5A pseudogenes map to 10q23.3, 17q25, and 19q13.2". Genomics. 25 (3): 749–752. doi:10.1016/0888-7543(95)80025-H. PMID   7759117.
6. Wolff EC, Kang KR, Kim YS, Park MH (August 2007). "Posttranslational synthesis of hypusine: evolutionary progression and specificity of the hypusine modification". Amino Acids. 33 (2): 341–350. doi:10.1007/s00726-007-0525-0. PMC   2572820 . PMID   17476569.
7. Park JH, Johansson HE, Aoki H, Huang BX, Kim HY, Ganoza MC, Park MH (January 2012). "Post-translational modification by β-lysylation is required for activity of Escherichia coli elongation factor P (EF-P)". The Journal of Biological Chemistry. 287 (4): 2579–2590. doi: 10.1074/jbc.M111.309633 . PMC   3268417 . PMID   22128152.
8. Peil L, Starosta AL, Virumäe K, Atkinson GC, Tenson T, Remme J, Wilson DN (August 2012). "Lys34 of translation elongation factor EF-P is hydroxylated by YfcM". Nature Chemical Biology. 8 (8): 695–697. doi:10.1038/nchembio.1001. PMID   22706199.
9. Rossi D, Kuroshu R, Zanelli CF, Valentini SR (2013). "eIF5A and EF-P: two unique translation factors are now traveling the same road". Wiley Interdisciplinary Reviews. RNA. 5 (2): 209–222. doi:10.1002/wrna.1211. PMID   24402910. S2CID   25447826.
10. Faundes V, Jennings MD, Crilly S, Legraie S, Withers SE, Cuvertino S, Davies SJ, Douglas AG, Fry AE, Harrison V, Amiel J, Lehalle D, Newman WG, Newkirk P, Ranells J, Splitt M, Cross LA, Saunders CJ, Sullivan BR, Granadillo JL, Gordon CT, Kasher PR, Pavitt GD, Banka S (February 2021). "Impaired eIF5A function causes a Mendelian disorder that is partially rescued in model systems by spermidine". Nature Communications. 12 (1): 833. Bibcode:2021NatCo..12..833F. doi:10.1038/s41467-021-21053-2. PMC   7864902 . PMID   33547280.
11. "OMIM Entry - # 619376 - FAUNDES-BANKA SYNDROME; FABAS". omim.org. Retrieved 2022-01-03.

Further reading

• Bevec D, Hauber J (1997). "Eukaryotic initiation factor 5A activity and HIV-1 Rev function". Biological Signals. 6 (3): 124–133. doi:10.1159/000109118. PMID   9285095.
• Li L, Li HS, Pauza CD, Bukrinsky M, Zhao RY (2006). "Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions". Cell Research. 15 (11–12): 923–934. doi: 10.1038/sj.cr.7290370 . PMID   16354571.
• Rasmussen HH, van Damme J, Puype M, Gesser B, Celis JE, Vandekerckhove J (December 1992). "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes". Electrophoresis. 13 (12): 960–969. doi:10.1002/elps.11501301199. PMID   1286667. S2CID   41855774.
• Chung SI, Park MH, Folk JE, Lewis MS (February 1991). "Eukaryotic initiation factor 5A: the molecular form of the hypusine-containing protein from human erythrocytes". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1076 (3): 448–451. doi:10.1016/0167-4838(91)90490-q. PMID   1900436.
• Smit-McBride Z, Dever TE, Hershey JW, Merrick WC (January 1989). "Sequence determination and cDNA cloning of eukaryotic initiation factor 4D, the hypusine-containing protein". The Journal of Biological Chemistry. 264 (3): 1578–1583. doi: 10.1016/S0021-9258(18)94226-2 . PMID   2492279.
• Park MH, Liu TY, Neece SH, Swiggard WJ (November 1986). "Eukaryotic initiation factor 4D. Purification from human red blood cells and the sequence of amino acids around its single hypusine residue". The Journal of Biological Chemistry. 261 (31): 14515–14519. doi: 10.1016/S0021-9258(18)66899-1 . PMID   3095320.
• Koettnitz K, Kappel B, Baumruker T, Hauber J, Bevec D (July 1994). "The genomic structure encoding human initiation factor eIF-5A". Gene. 144 (2): 249–252. doi:10.1016/0378-1119(94)90385-9. PMID   7545941.
• Klier H, Csonga R, Joäo HC, Eckerskorn C, Auer M, Lottspeich F, Eder J (November 1995). "Isolation and structural characterization of different isoforms of the hypusine-containing protein eIF-5A from HeLa cells". Biochemistry. 34 (45): 14693–14702. doi:10.1021/bi00045a010. PMID   7578077.
• Koettnitz K, Wöhl T, Kappel B, Lottspeich F, Hauber J, Bevec D (July 1995). "Identification of a new member of the human eIF-5A gene family". Gene. 159 (2): 283–284. doi:10.1016/0378-1119(95)00136-T. PMID   7622067.
• Joe YA, Park MH (October 1994). "Structural features of the eIF-5A precursor required for posttranslational synthesis of deoxyhypusine". The Journal of Biological Chemistry. 269 (41): 25916–25921. doi: 10.1016/S0021-9258(18)47333-4 . PMID   7929297.
• Bevec D, Klier H, Holter W, Tschachler E, Valent P, Lottspeich F, et al. (November 1994). "Induced gene expression of the hypusine-containing protein eukaryotic initiation factor 5A in activated human T lymphocytes". Proceedings of the National Academy of Sciences of the United States of America. 91 (23): 10829–10833. Bibcode:1994PNAS...9110829B. doi: 10.1073/pnas.91.23.10829 . PMC   45119 . PMID   7971969.
• Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–174. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Ruhl M, Himmelspach M, Bahr GM, Hammerschmid F, Jaksche H, Wolff B, et al. (December 1993). "Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activation". The Journal of Cell Biology. 123 (6 Pt 1): 1309–1320. doi:10.1083/jcb.123.6.1309. PMC   2290910 . PMID   8253832.
• Liu YP, Nemeroff M, Yan YP, Chen KY (1997). "Interaction of eukaryotic initiation factor 5A with the human immunodeficiency virus type 1 Rev response element RNA and U6 snRNA requires deoxyhypusine or hypusine modification". Biological Signals. 6 (3): 166–174. doi:10.1159/000109123. PMID   9285100.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Singh US, Li Q, Cerione R (January 1998). "Identification of the eukaryotic initiation factor 5A as a retinoic acid-stimulated cellular binding partner for tissue transglutaminase II". The Journal of Biological Chemistry. 273 (4): 1946–1950. doi: 10.1074/jbc.273.4.1946 . PMID   9442029.
• Schatz O, Oft M, Dascher C, Schebesta M, Rosorius O, Jaksche H, et al. (February 1998). "Interaction of the HIV-1 rev cofactor eukaryotic initiation factor 5A with ribosomal protein L5". Proceedings of the National Academy of Sciences of the United States of America. 95 (4): 1607–1612. Bibcode:1998PNAS...95.1607S. doi: 10.1073/pnas.95.4.1607 . PMC   19115 . PMID   9465063.
• Lee YB, Joe YA, Wolff EC, Dimitriadis EK, Park MH (May 1999). "Complex formation between deoxyhypusine synthase and its protein substrate, the eukaryotic translation initiation factor 5A (eIF5A) precursor". The Biochemical Journal. 340 (1): 273–281. doi:10.1042/0264-6021:3400273. PMC   1220246 . PMID   10229683.