Ribosomal protein SA

RPSA
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3BCH, 4UG0, 4V6X, 5A2Q, 5AJ0, 4UJE, 4D5L, 4UJD, 5FLX, 4D61
Identifiers
Aliases	RPSA , 37LRP, 67LR, ICAS, LAMBR, LAMR1, LBP, LBP/p40, LRP, LRP/LR, NEM/1CHD4, SA, lamR, p40, Ribosomal protein SA
External IDs	OMIM: 150370 MGI: 105381 HomoloGene: 68249 GeneCards: RPSA
Gene location (Human) Chr. Chromosome 3 (human) [1] Band 3p22.1 Start 39,406,716 bp [1] End 39,412,542 bp [1]
Gene location (Mouse) Chr. Chromosome 9 (mouse) [2] Band 9 F4|9 71.41 cM Start 119,956,755 bp [2] End 119,961,435 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right uterine tube ganglionic eminence islet of Langerhans body of pancreas spleen lymph node nucleus accumbens body of stomach skin of abdomen prostate Top expressed in ganglionic eminence uterus spleen bone marrow mirror thymus islet of Langerhans zone of skin lip urinary bladder More reference expression data BioGPS n/a
Gene ontology Molecular function virus receptor activity structural constituent of ribosome ribosome binding laminin receptor activity protein binding RNA binding laminin binding Cellular component cytoplasm cytosol ribosome membrane plasma membrane intracellular anatomical structure small ribosomal subunit extracellular exosome nucleus 90S preribosome nucleoplasm cytosolic small ribosomal subunit Biological process endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) viral transcription SRP-dependent cotranslational protein targeting to membrane translational initiation rRNA export from nucleus viral entry into host cell nuclear-transcribed mRNA catabolic process, nonsense-mediated decay protein biosynthesis viral process rRNA processing ribosomal small subunit assembly cell adhesion cytoplasmic translation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 3921 16785 Ensembl ENSG00000168028 ENSMUSG00000032518 UniProt P08865 P14206 RefSeq (mRNA) NM_002295 NM_001304288 NM_011029 RefSeq (protein) NP_001291217 NP_002286 NP_035159 Location (UCSC) Chr 3: 39.41 – 39.41 Mb Chr 9: 119.96 – 119.96 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

40S ribosomal protein SA is a ribosomal protein that in humans is encoded by the RPSA gene. ^{[5] [6]} It also acts as a cell surface receptor, in particular for laminin, and is involved in several pathogenic processes.

Function

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. The RPSA gene encodes a multifunctional protein, which is both a ribosomal protein and a high-affinity, non-integrin laminin receptor. This protein has been variously called Ribosomal protein SA; RPSA; LamR; LamR1; 37 kDa Laminin Receptor Precursor; 37LRP; 67 kDa Laminin Receptor; 67LR; 37/67 kDa Laminin Receptor; LRP/LR; LBP/p40; and p40 ribosome-associated protein. Ribosomal protein SA and RPSA are the approved name and symbol. The amino acid sequence of RPSA is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of RPSA transcript is higher in colon carcinoma tissue and lung cancer cell lines than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of the RPSA gene exist; however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. ^[7]

Structure and stability

The complementary DNA (cDNA) of the RPSA gene is formed by the assembly of seven exons, six of which correspond to the coding sequence. ^[6] The amino acid sequence of RPSA, deduced from the sequence of its cDNA, includes 295 residues. RPSA can be sub-divided in two main domains: an N-domain (residues 1-209), which corresponds to exons 2-5 of the gene, and a C-domain (residues 210-295), which corresponds to exons 6-7. The N-domain of RPSA is homologous to the ribosomal protein S2 (RPS2) of prokaryotes. It contains a palindromic sequence 173LMWWML178 which is conserved in all metazoans. Its C-domain is highly conserved in vertebrates. The amino acid sequence of RPSA is 98% identical in all mammals. RPSA is a ribosomal protein which has acquired the function of laminin receptor during evolution. ^{[8] [9]} The structure of the N-domain of RPSA is similar to those of prokaryotic RPS2. ^[10] The C-domain is intrinsically disordered in solution. The N-domain is monomeric in solution and unfolds according to a three state equilibrium. The folding intermediate is predominant at 37 °C. ^[11]

Interactions

Several interactions of RPSA that had originally been discovered by methods of cellular biology, have subsequently been confirmed by using recombinant derivatives and in vitro experiments. The latter have shown that the folded N-domain and disordered C-domain of RPSA have both common and specific functions. ^[12]

• RPSA binds to proteins that are involved in the translation of the genetic code. (i) Yeast two-hybrid screens have shown that RPSA binds to Ribosomal protein S21 of the 40S small ribosomal subunit. ^{[13] [14]} (ii) Serial deletions of RPSA have shown that the segment of residues 236-262, included in the C-domain, is involved in the interaction between RPSA and the 40S subunit of ribosome. ^[15] (iii) Studies that were based on nuclear magnetic resonance spectroscopy (NMR), have shown that the anticodon binding domain of Lysyl-tRNA synthetase binds directly to the C-domain of RPSA. ^[16]
• RPSA was initially identified as a laminin binding protein. ^{[17] [18]} Both recombinant N-domain and C-domain of RPSA bind laminin in vitro, and they bind with similar dissociation constants (300 nM). ^{[10] [12]}
• Both RPSA and laminin belong to the heparin/heparan sulfate interactome. ^[19] Heparin binds in vitro to the N-domain of RPSA but not to its C-domain. Moreover, the C-domain of RPSA and heparin compete for binding to laminin, which shows that the highly acidic C-domain of RPSA mimicks heparin (and potentially heparan sulfates) for the binding to laminin. ^[12]
• RPSA is a potential cellular receptor for several pathogenic Flaviviruses, including the dengue virus (DENV), ^{[20] [21]} and Alphaviruses, including the Sindbis virus (SINV). ^[22] The N-domain of RPSA includes a binding site for SINV in vitro. ^[10] The N-domain also includes weak binding sites for recombinant domain 3 (ED3, residues 296-400) from the envelope proteins of two Flaviviruses, West-Nile virus and serotype 2 of DENV. The C-domain includes weak binding sites for domain 3 of the yellow fever virus (YFV) and of serotypes 1 and 2 of DENV. In contrast, domain 3 from the Japanese encephalitis virus does not appear to bind RPSA in vitro. ^[12]
• RPSA is also a receptor for small molecules. (i) RPSA binds aflatoxin B1 both in vivo and in vitro. ^[23] (ii) RPSA is a receptor for epigallocatechin-gallate (EGCG), which is a major constituent of green tea and has many health related effects. ^{[24] [25]} EGCG binds only to the N-domain of RPSA in vitro, with a dissociation constant of 100 nM, but not to its C-domain. ^[12]

References

1. GRCh38: Ensembl release 89: ENSG00000168028 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000032518 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Satoh K, Narumi K, Sakai T, Abe T, Kikuchi T, Matsushima K, Sindoh S, Motomiya M (Jul 1992). "Cloning of 67-kDa laminin receptor cDNA and gene expression in normal and malignant cell lines of the human lung". Cancer Lett. 62 (3): 199–203. doi:10.1016/0304-3835(92)90096-E. PMID   1534510.
6. Jackers P, Minoletti F, Belotti D, Clausse N, Sozzi G, Sobel ME, Castronovo V (Sep 1996). "Isolation from a multigene family of the active human gene of the metastasis-associated multifunctional protein 37LRP/p40 at chromosome 3p21.3". Oncogene. 13 (3): 495–503. PMID   8760291.
7. DiGiacomo, Vincent; Meruelo, Daniel (May 2016). "Looking into laminin receptor: critical discussion regarding the non-integrin 37/67-kDa laminin receptor/RPSA protein". Biological Reviews. 91 (2): 288–310. doi:10.1111/brv.12170. PMC   5249262 . PMID   25630983.
8. Ardini E, Pesole G, Tagliabue E, Magnifico A, Castronovo V, Sobel ME, Colnaghi MI, Ménard S (August 1998). "The 67-kDa laminin receptor originated from a ribosomal protein that acquired a dual function during evolution". Molecular Biology and Evolution. 15 (8): 1017–25. doi: 10.1093/oxfordjournals.molbev.a026000 . PMID   9718729.
9. Nelson J, McFerran NV, Pivato G, Chambers E, Doherty C, Steele D, Timson DJ (February 2008). "The 67 kDa laminin receptor: structure, function and role in disease". Bioscience Reports. 28 (1): 33–48. doi:10.1042/BSR20070004. PMID   18269348.
10. Jamieson KV, Wu J, Hubbard SR, Meruelo D (February 2008). "Crystal structure of the human laminin receptor precursor". The Journal of Biological Chemistry. 283 (6): 3002–5. doi: 10.1074/jbc.C700206200 . PMID   18063583.
11. Ould-Abeih, MB; Petit-Topin, I; Zidane, N; Baron, B; Bedouelle, Hugues (Jun 2012). "Multiple folding states and disorder of ribosomal protein SA, a membrane receptor for laminin, anticarcinogens, and pathogens". Biochemistry. 51 (24): 4807–4821. doi:10.1021/bi300335r. PMID   22640394.
12. Zidane, N; Ould-Abeih, MB; Petit-Topin, I; Bedouelle, H (2012). "The folded and disordered domains of human ribosomal protein SA have both idiosyncratic and shared functions as membrane receptors". Biosci. Rep. 33 (1): 113–124. doi:10.1042/BSR20120103. PMC   4098866 . PMID   23137297.
13. Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (Sep 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–968. doi:10.1016/j.cell.2005.08.029. hdl: 11858/00-001M-0000-0010-8592-0 . PMID   16169070. S2CID   8235923.
14. Sato M, Saeki Y, Tanaka K, Kaneda Y (Mar 1999). "Ribosome-associated protein LBP/p40 binds to S21 protein of 40S ribosome: analysis using a yeast two-hybrid system". Biochem. Biophys. Res. Commun. 256 (2): 385–390. doi:10.1006/bbrc.1999.0343. PMID   10079194.
15. Malygin, AA; Babaylova, ES; Loktev, VB; Karpova, GG (2011). "A region in the C-terminal domain of ribosomal protein SA required for binding of SA to the human 40S ribosomal subunit". Biochimie. 93 (3): 612–617. doi:10.1016/j.biochi.2010.12.005. PMID   21167900.
16. Cho, HY; Ul Mushtaq, A; Lee, JY; Kim, DG; Seok, MS; Jang, M; Han, BW; Kim, S; Jeon, YH (2014). "Characterization of the interaction between lysyl-tRNA synthetase and laminin receptor by NMR". FEBS Lett. 588 (17): 2851–2858. doi:10.1016/j.febslet.2014.06.048. PMID   24983501. S2CID   36128866.
17. Rao, NC; Barsky, SH; Terranova, VP; Liotta, LA (1983). "Isolation of a tumor cell laminin receptor". Biochem. Biophys. Res. Commun. 111 (3): 804–808. doi:10.1016/0006-291X(83)91370-0. PMID   6301485.
18. Lesot, H; Kühl, U; Mark, K (1983). "Isolation of a laminin-binding protein from muscle cell membranes". EMBO J. 2 (6): 861–865. doi:10.1002/j.1460-2075.1983.tb01514.x. PMC   555201 . PMID   16453457.
19. Ori, A; Wilkinson, MC; Fernig, DG (2011). "A systems biology approach for the investigation of the heparin/heparan sulfate interactome". J. Biol. Chem. 286 (22): 19892–19904. doi: 10.1074/jbc.M111.228114 . PMC   3103365 . PMID   21454685.
20. Thepparit, C; Smith, DR (2004). "Serotype-specific entry of dengue virus into liver cells: identification of the 37-kilodalton/67-kilodalton high-affinity laminin receptor as a dengue virus serotype 1 receptor". J. Virol. 78 (22): 12647–12656. doi:10.1128/jvi.78.22.12647-12656.2004. PMC   525075 . PMID   15507651.
21. Tio, PH; Jong, WW; Cardosa, MJ (2005). "Two dimensional VOPBA reveals laminin receptor (LAMR1) interaction with dengue virus serotypes 1, 2 and 3". Virol. J. 2: 25. doi:10.1186/1743-422X-2-25. PMC   1079963 . PMID   15790424.
22. Wang, KS; Kuhn, RJ; Strauss, EG; Ou, S; Strauss, JH (1992). "High-affinity laminin receptor is a receptor for Sindbis virus in mammalian cells". J. Virol. 66 (8): 4992–5001. doi:10.1128/JVI.66.8.4992-5001.1992. PMC   241351 . PMID   1385835.
23. Zhuang, Z; Huang, Y; Yang, Y; Wang, S (2016). "Identification of AFB1-interacting proteins and interactions between RPSA and AFB1". J. Hazard. Mater. 301: 297–303. doi:10.1016/j.jhazmat.2015.08.053. PMID   26372695.
24. Tachibana, H; Koga, K; Fujimura, Y; Yamada, K (2004). "A receptor for green tea polyphenol EGCG". Nat. Struct. Mol. Biol. 11 (4): 380–381. doi:10.1038/nsmb743. PMID   15024383. S2CID   27868813.
25. Tachibana, H (2011). "Green tea polyphenol sensing". Proc. Jpn. Acad. Ser. B Phys. Biol. Sci. 87 (3): 66–80. Bibcode:2011PJAB...87...66T. doi:10.2183/pjab.87.66. PMC   3066547 . PMID   21422740.

Further reading

• Belkin AM, Stepp MA (2000). "Integrins as receptors for laminins". Microsc. Res. Tech. 51 (3): 280–301. doi:10.1002/1097-0029(20001101)51:3<280::AID-JEMT7>3.0.CO;2-O. PMID   11054877. S2CID   45941383.
• Wewer UM, Liotta LA, Jaye M, Ricca GA, Drohan WN, Claysmith AP, Rao CN, Wirth P, Coligan JE, Albrechtsen R (1986). "Altered levels of laminin receptor mRNA in various human carcinoma cells that have different abilities to bind laminin". Proc. Natl. Acad. Sci. U.S.A. 83 (19): 7137–7141. Bibcode:1986PNAS...83.7137W. doi: 10.1073/pnas.83.19.7137 . PMC   386670 . PMID   2429301.
• Van den Ouweland AM, Van Duijnhoven HL, Deichmann KA, Van Groningen JJ, de Leij L, Van de Ven WJ (1989). "Characteristics of a multicopy gene family predominantly consisting of processed pseudogenes". Nucleic Acids Res. 17 (10): 3829–3843. doi:10.1093/nar/17.10.3829. PMC   317862 . PMID   2543954.
• Yow HK, Wong JM, Chen HS, Lee CG, Davis S, Steele GD, Chen LB (1988). "Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full-length cDNA encoding the protein" (PDF). Proc. Natl. Acad. Sci. U.S.A. 85 (17): 6394–6398. Bibcode:1988PNAS...85.6394Y. doi: 10.1073/pnas.85.17.6394 . PMC   281978 . PMID   2970639.
• Selvamurugan N, Eliceiri GL (1996). "The gene for human E2 small nucleolar RNA resides in an intron of a laminin-binding protein gene". Genomics. 30 (2): 400–1. PMID   8586453.
• Vladimirov SN, Ivanov AV, Karpova GG, Musolyamov AK, Egorov TA, Thiede B, Wittmann-Liebold B, Otto A (1996). "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry". Eur. J. Biochem. 239 (1): 144–149. doi: 10.1111/j.1432-1033.1996.0144u.x . PMID   8706699.
• Clausse N, Jackers P, Jarès P, Joris B, Sobel ME, Castronovo V (1997). "Identification of the active gene coding for the metastasis-associated 37LRP/p40 multifunctional protein". DNA Cell Biol. 15 (12): 1009–1023. doi:10.1089/dna.1996.15.1009. PMID   8985115.
• Daidone MG, Silvestrini R, Benini E, Grigioni WF, D'Errico A (1997). "Expression of high-affinity 67-kDa laminin receptors in primary breast cancers and metachronous metastatic lesions or contralateral cancers". Br. J. Cancer. 76 (1): 52–3. doi:10.1038/bjc.1997.335. PMC   2223804 . PMID   9218732.
• Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (1998). "A map of 75 human ribosomal protein genes". Genome Res. 8 (5): 509–23. doi: 10.1101/gr.8.5.509 . PMID   9582194.
• de Manzoni G, Guglielmi A, Verlato G, Tomezzoli A, Pelosi G, Schiavon I, Cordiano C (1998). "Prognostic significance of 67-kDa laminin receptor expression in advanced gastric cancer". Oncology. 55 (5): 456–460. doi:10.1159/000011895. PMID   9732225. S2CID   46799166.
• Sato M, Saeki Y, Tanaka K, Kaneda Y (1999). "Ribosome-associated protein LBP/p40 binds to S21 protein of 40S ribosome: analysis using a yeast two-hybrid system". Biochem. Biophys. Res. Commun. 256 (2): 385–390. doi:10.1006/bbrc.1999.0343. PMID   10079194.
• Canfield SM, Khakoo AY (1999). "The nonintegrin laminin binding protein (p67 LBP) is expressed on a subset of activated human T lymphocytes and, together with the integrin very late activation antigen-6, mediates avid cellular adherence to laminin". J. Immunol. 163 (6): 3430–40. PMID   10477615.
• Donaldson EA, McKenna DJ, McMullen CB, Scott WN, Stitt AW, Nelson J (2000). "The expression of membrane-associated 67-kDa laminin receptor (67LR) is modulated in vitro by cell-contact inhibition". Mol. Cell Biol. Res. Commun. 3 (1): 53–59. doi:10.1006/mcbr.2000.0191. PMID   10683318.
• Pedraza C, Geberhiwot T, Ingerpuu S, Assefa D, Wondimu Z, Kortesmaa J, Tryggvason K, Virtanen I, Patarroyo M (2000). "Monocytic cells synthesize, adhere to, and migrate on laminin-8 (alpha 4 beta 1 gamma 1)". J. Immunol. 165 (10): 5831–8. doi: 10.4049/jimmunol.165.10.5831 . PMID   11067943.
• Vande Broek I, Vanderkerken K, De Greef C, Asosingh K, Straetmans N, Van Camp B, Van Riet I (2001). "Laminin-1-induced migration of multiple myeloma cells involves the high-affinity 67 kD laminin receptor". Br. J. Cancer. 85 (9): 1387–1395. doi:10.1054/bjoc.2001.2078. PMC   2375239 . PMID   11720479.
• Waltregny D, de Leval L, Coppens L, Youssef E, de Leval J, Castronovo V (2002). "Detection of the 67-kD laminin receptor in prostate cancer biopsies as a predictor of recurrence after radical prostatectomy". Eur. Urol. 40 (5): 495–503. doi:10.1159/000049825. PMID   11752855. S2CID   22778829.