40S ribosomal protein S27

RPS27
Available structures PDB Human UniProt search: PDBe RCSB List of PDB id codes 4UG0, 4V6X, 5A2Q, 5AJ0, 4KZY, 3J7R, 4D61, 4KZX, 4D5L, 5FLX, 4UJD, 3J7P, 4KZZ, 4UJE, 4UJC
Identifiers
Aliases	RPS27 , MPS-1, MPS1, S27, ribosomal protein S27, DBA17
External IDs	OMIM: 603702 HomoloGene: 803 GeneCards: RPS27
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1q21.3 Start 153,990,762 bp [1] End 153,992,155 bp [1]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ganglionic eminence canal of the cervix lymph node Achilles tendon uterine tube corpus callosum gastric mucosa right uterine tube left uterine tube left lobe of thyroid gland n/a More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding structural constituent of ribosome zinc ion binding metal ion binding protein binding RNA binding Cellular component cytosol ribosome intracellular anatomical structure nucleus nucleoplasm cytosolic small ribosomal subunit postsynaptic density presynapse glutamatergic synapse GABA-ergic synapse Biological process viral transcription SRP-dependent cotranslational protein targeting to membrane translational initiation ribosomal small subunit assembly nuclear-transcribed mRNA catabolic process, nonsense-mediated decay cell population proliferation protein biosynthesis sister chromatid cohesion rRNA processing Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 6232 n/a Ensembl ENSG00000177954 n/a UniProt P42677 n/a RefSeq (mRNA) NM_001030 NM_001349946 NM_001349947 n/a RefSeq (protein) NP_001021 NP_001336875 NP_001336876 n/a Location (UCSC) Chr 1: 153.99 – 153.99 Mb n/a PubMed search [2] n/a
Wikidata
View/Edit Human

40S ribosomal protein S27, also known as metallopan-stimulin 1 or MPS-1, is a protein that in humans is encoded by the RPS27 gene. ^{[3] [4] [5]} Metallopanstimulin is a zinc finger protein proposed to be involved DNA repair as well as oncogenesis. ^[6]

Function

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. ^[5]

Clinical significance

Its expression is increased in several types of malignancy and MPS levels have been reported to drop with treatment of some cancers. It has also been used as a target for some chemotherapies, which aim to chelate out the zinc from the zinc finger motif of the MPS, thus yielding it inactive. These therapies have shown promise for the treatment of cancer in laboratory experiments and some limited clinical trials. Head and neck cancer transfected to overexpress this protein have demonstrated suppressed growth. ^[6]

References

1. GRCh38: Ensembl release 89: ENSG00000177954 - Ensembl, May 2017
2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
3. Tsui SK, Lee SM, Fung KP, Waye MM, Lee CY (Oct 1996). "Primary structures and sequence analysis of human ribosomal proteins L39 and S27". Biochemistry and Molecular Biology International. 40 (3): 611–6. doi: 10.1080/15216549600201203 . PMID   8908372. S2CID   7909023.
4. Fernandez-Pol JA, Klos DJ, Hamilton PD (Oct 1993). "A growth factor-inducible gene encodes a novel nuclear protein with zinc finger structure". The Journal of Biological Chemistry. 268 (28): 21198–204. doi: 10.1016/S0021-9258(19)36910-8 . PMID   8407955.
5. "Entrez Gene: RPS27 ribosomal protein S27 (metallopanstimulin 1)".
6. Fernandez-Pol JA (1996). "Metallopanstimulin as a novel tumor marker in sera of patients with various types of common cancers: implications for prevention and therapy". Anticancer Research. 16 (4B): 2177–85. PMID   8694540.

Further reading

• Scurry WC, Stack BC (Dec 2007). "Role of metalloproteins in the clinical management of head and neck squamous cell carcinoma". Head & Neck. 29 (12): 1144–55. CiteSeerX   10.1.1.610.1189 . doi:10.1002/hed.20655. PMID   17657798. S2CID   11534394.
• Stack BC, Hollenbeak CS, Lee CM, Dunphy FR, Lowe VJ, Hamilton PD (December 2004). "Metallopanstimulin as a marker for head and neck cancer". World Journal of Surgical Oncology. 2: 45. doi: 10.1186/1477-7819-2-45 . PMC   544581 . PMID   15598348.
• Lee WJ, Keefer K, Hollenbeak CS, Stack BC (Oct 2004). "A new assay to screen for head and neck squamous cell carcinoma using the tumor marker metallopanstimulin". Otolaryngology–Head and Neck Surgery. 131 (4): 466–71. doi:10.1016/j.otohns.2004.03.011. PMID   15467619. S2CID   22629299.
• Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal proteins". Biochemistry and Cell Biology. 73 (11–12): 933–47. doi:10.1139/o95-101. PMID   8722009.
• Fernandez-Pol JA, Klos DJ, Hamilton PD (Aug 1994). "Metallopanstimulin gene product produced in a baculovirus expression system is a nuclear phosphoprotein that binds to DNA". Cell Growth & Differentiation. 5 (8): 811–25. PMID   7986747.
• Vladimirov SN, Ivanov AV, Karpova GG, Musolyamov AK, Egorov TA, Thiede B, Wittmann-Liebold B, Otto A (Jul 1996). "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry". European Journal of Biochemistry. 239 (1): 144–9. doi: 10.1111/j.1432-1033.1996.0144u.x . PMID   8706699.
• Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (May 1998). "A map of 75 human ribosomal protein genes". Genome Research. 8 (5): 509–23. doi: 10.1101/gr.8.5.509 . PMID   9582194.
• Yoshihama M, Uechi T, Asakawa S, Kawasaki K, Kato S, Higa S, Maeda N, Minoshima S, Tanaka T, Shimizu N, Kenmochi N (Mar 2002). "The human ribosomal protein genes: sequencing and comparative analysis of 73 genes". Genome Research. 12 (3): 379–90. doi:10.1101/gr.214202. PMC   155282 . PMID   11875025.
• Atsuta Y, Aoki N, Sato K, Oikawa K, Nochi H, Miyokawa N, Hirata S, Kimura S, Sasajima T, Katagiri M (Aug 2002). "Identification of metallopanstimulin-1 as a member of a tumor associated antigen in patients with breast cancer". Cancer Letters. 182 (1): 101–7. doi:10.1016/S0304-3835(02)00068-X. PMID   12175529.
• Martin-Lluesma S, Stucke VM, Nigg EA (Sep 2002). "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2". Science. 297 (5590): 2267–70. Bibcode:2002Sci...297.2267M. doi:10.1126/science.1075596. PMID   12351790. S2CID   7879023.
• Liu ST, Chan GK, Hittle JC, Fujii G, Lees E, Yen TJ (Apr 2003). "Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores". Molecular Biology of the Cell. 14 (4): 1638–51. doi:10.1091/mbc.02-05-0074. PMC   153128 . PMID   12686615.
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (Feb 2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nature Cell Biology. 6 (2): 97–105. doi:10.1038/ncb1086. PMID   14743216. S2CID   11683986.
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (Jan 2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID   15635413. S2CID   4344740.
• Yu Y, Ji H, Doudna JA, Leary JA (Jun 2005). "Mass spectrometric analysis of the human 40S ribosomal subunit: native and HCV IRES-bound complexes". Protein Science. 14 (6): 1438–46. doi:10.1110/ps.041293005. PMC   2253395 . PMID   15883184.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Bhonde MR, Hanski ML, Budczies J, Cao M, Gillissen B, Moorthy D, Simonetta F, Scherübl H, Truss M, Hagemeier C, Mewes HW, Daniel PT, Zeitz M, Hanski C (Mar 2006). "DNA damage-induced expression of p53 suppresses mitotic checkpoint kinase hMps1: the lack of this suppression in p53MUT cells contributes to apoptosis". The Journal of Biological Chemistry. 281 (13): 8675–85. doi: 10.1074/jbc.M511333200 . PMID   16446370.
• Wang YW, Qu Y, Li JF, Chen XH, Liu BY, Gu QL, Zhu ZG (Aug 2006). "In vitro and in vivo evidence of metallopanstimulin-1 in gastric cancer progression and tumorigenicity". Clinical Cancer Research. 12 (16): 4965–73. doi: 10.1158/1078-0432.CCR-05-2316 . PMID   16914586.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi: 10.1016/j.cell.2006.09.026 . PMID   17081983. S2CID   7827573.