Keratin 7

KRT7
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4XIF
Identifiers
Aliases	KRT7 , CK7, K2C7, K7, SCL, keratin 7
External IDs	OMIM: 148059; MGI: 96704; HomoloGene: 4058; GeneCards: KRT7; OMA:KRT7 - orthologs
Gene location (Human) Chr. Chromosome 12 (human) [1] Band 12q13.13 Start 52,232,520 bp [1] End 52,252,186 bp [1]
Gene location (Mouse) Chr. Chromosome 15 (mouse) [2] Band 15 F1- F2|15 56.88 cM Start 101,308,924 bp [2] End 101,328,194 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in left lobe of thyroid gland right lobe of thyroid gland olfactory zone of nasal mucosa upper lobe of left lung right lung islet of Langerhans right uterine tube gallbladder minor salivary glands placenta Top expressed in transitional epithelium of urinary bladder right lung lobe left lung left lung lobe crypt of lieberkuhn of small intestine conjunctival fornix submandibular gland cervix pyloric antrum efferent ductule More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding structural molecule activity Cellular component cytoplasm keratin filament extracellular exosome intermediate filament nucleus cytosol Biological process viral process keratinization cornification Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 3855 110310 Ensembl ENSG00000135480 ENSMUSG00000023039 UniProt P08729 Q9DCV7 RefSeq (mRNA) NM_005556 NM_033073 RefSeq (protein) NP_005547 NP_149064 Location (UCSC) Chr 12: 52.23 – 52.25 Mb Chr 15: 101.31 – 101.33 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Keratin, type II cytoskeletal 7 also known as cytokeratin-7 (CK-7) or keratin-7 (K7) or sarcolectin (SCL) is a protein that in humans is encoded by the KRT7 gene. ^{[5] [6] [7]} Keratin 7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

Function

Keratin-7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. ^[7]

Keratin-7 is found in simple glandular epithelia, and in transitional epithelium. Epithelial cells of the lung and breast both contain keratin-7, but some other glandular epithelia, such as those of the colon and prostate, do not.

Immunohistochemistry

Immunohistochemistry for CK7 of a metastatic undifferentiated carcinoma to a lymph node.

Because the keratin-7 antigen is found in both healthy and neoplastic cells, antibodies to CK7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas (staining positive) from colonic and prostate cancers (negative), respectively. It is commonly used together with CK20 when making such diagnoses. ^[8]

References

1. GRCh38: Ensembl release 89: ENSG00000135480 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000023039 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Rosenberg M, Fuchs E, Le Beau MM, Eddy RL, Shows TB (Aug 1991). "Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12". Cytogenetics and Cell Genetics. 57 (1): 33–8. doi:10.1159/000133109. PMID   1713141.
6. Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW (Jul 2006). "New consensus nomenclature for mammalian keratins". The Journal of Cell Biology. 174 (2): 169–74. doi:10.1083/jcb.200603161. PMC   2064177 . PMID   16831889.
7. "Entrez Gene: KRT7 keratin 7".
8. Leong, Anthony S-Y; Cooper, Kumarason; Leong, F Joel W-M (2003). Manual of Diagnostic Cytology (2 ed.). Greenwich Medical Media, Ltd. p. 173. ISBN   978-1-84110-100-2.

Further reading

• Bouwens L (Mar 1998). "Cytokeratins and cell differentiation in the pancreas". The Journal of Pathology. 184 (3): 234–9. doi:10.1002/(SICI)1096-9896(199803)184:3<234::AID-PATH28>3.0.CO;2-D. PMID   9614373. S2CID   19443756.
• Glass C, Kim KH, Fuchs E (Dec 1985). "Sequence and expression of a human type II mesothelial keratin". The Journal of Cell Biology. 101 (6): 2366–73. doi:10.1083/jcb.101.6.2366. PMC   2114014 . PMID   2415537.
• Ramaekers F, Huysmans A, Schaart G, Moesker O, Vooijs P (May 1987). "Tissue distribution of keratin 7 as monitored by a monoclonal antibody". Experimental Cell Research. 170 (1): 235–49. doi:10.1016/0014-4827(87)90133-9. PMID   2436934.
• Glass C, Fuchs E (Oct 1988). "Isolation, sequence, and differential expression of a human K7 gene in simple epithelial cells". The Journal of Cell Biology. 107 (4): 1337–50. doi:10.1083/jcb.107.4.1337. PMC   2115265 . PMID   2459129.
• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Gress TM, Müller-Pillasch F, Geng M, Zimmerhackl F, Zehetner G, Friess H, Büchler M, Adler G, Lehrach H (Oct 1996). "A pancreatic cancer-specific expression profile". Oncogene. 13 (8): 1819–30. PMID   8895530.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Alvarez Alvarez C, Sánchez Merino JM, Busto Castañón L, Pombo Felipe F, Arnal Monreal F (Jun 1998). "[Mucinous adenocarcinoma of the urachus synchronic with colorectal adenocarcinoma. Value of immunohistochemistry in the differential diagnosis]". Actas Urologicas Españolas. 22 (6): 515–8. PMID   9734130.
• Kaba A, Jiang PH, Chany-Fournier F, Chany C (Jul 1999). "Sarcolectin (SCL): structure and expression of the recombinant molecule". Biochimie. 81 (7): 709–15. doi:10.1016/S0300-9084(99)80128-X. PMID   10492017.
• Lin L, Holbro T, Alonso G, Gerosa D, Burger MM (2001). "Molecular interaction between human tumor marker protein p150, the largest subunit of eIF3, and intermediate filament protein K7". Journal of Cellular Biochemistry. 80 (4): 483–90. doi:10.1002/1097-4644(20010315)80:4<483::AID-JCB1002>3.0.CO;2-B. PMID   11169732. S2CID   35848129.
• Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y (Oct 2001). "Protein-protein interaction panel using mouse full-length cDNAs". Genome Research. 11 (10): 1758–65. doi:10.1101/gr.180101. PMC   311163 . PMID   11591653.
• Harris RA, Yang A, Stein RC, Lucy K, Brusten L, Herath A, Parekh R, Waterfield MD, O'Hare MJ, Neville MA, Page MJ, Zvelebil MJ (Feb 2002). "Cluster analysis of an extensive human breast cancer cell line protein expression map database". Proteomics. 2 (2): 212–23. doi:10.1002/1615-9861(200202)2:2<212::AID-PROT212>3.0.CO;2-H. PMID   11840567. S2CID   44946014.
• Bonvini P, Gastaldi T, Falini B, Rosolen A (Mar 2002). "Nucleophosmin-anaplastic lymphoma kinase (NPM-ALK), a novel Hsp90-client tyrosine kinase: down-regulation of NPM-ALK expression and tyrosine phosphorylation in ALK(+) CD30(+) lymphoma cells by the Hsp90 antagonist 17-allylamino,17-demethoxygeldanamycin". Cancer Research. 62 (5): 1559–66. PMID   11888936.
• Jovanovic I, Tzardi M, Mouzas IA, Micev M, Pesko P, Milosavljevic T, Zois M, Sganzos M, Delides G, Kanavaros P (Apr 2002). "Changing pattern of cytokeratin 7 and 20 expression from normal epithelium to intestinal metaplasia of the gastric mucosa and gastroesophageal junction". Histology and Histopathology. 17 (2): 445–54. PMID   11962749.
• Kanduc D (Jul 2002). "Translational regulation of human papillomavirus type 16 E7 mRNA by the peptide SEQIKA, shared by rabbit alpha(1)-globin and human cytokeratin 7". Journal of Virology. 76 (14): 7040–8. doi:10.1128/JVI.76.14.7040-7048.2002. PMC   136328 . PMID   12072504.
• Smith FJ, Porter RM, Corden LD, Lunny DP, Lane EB, McLean WH (Oct 2002). "Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse". Biochemical and Biophysical Research Communications. 297 (4): 818–27. doi:10.1016/S0006-291X(02)02288-X. PMID   12359226.
• Blagoev B, Kratchmarova I, Ong SE, Nielsen M, Foster LJ, Mann M (Mar 2003). "A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling". Nature Biotechnology. 21 (3): 315–8. doi:10.1038/nbt790. PMID   12577067. S2CID   26838266.