Cantharidin

Cantharidin

Names
Preferred IUPAC name (3aR,4S,7R,7aS)-3a,7a-Dimethylhexahydro-4,7-epoxy[2]benzofuran-1,3-dione
Other names Cantharidin Spanish fly Ycanth
Identifiers
CAS Number	56-25-7  N
3D model (JSmol)	Interactive image
Beilstein Reference	85302
ChEBI	CHEBI:64213
ChEMBL	ChEMBL48449  N
ChemSpider	2297293  Y
ECHA InfoCard	100.000.240
EC Number	200-263-3
KEGG	C16778  N
PubChem CID	5944
UNII	IGL471WQ8P  Y
CompTox Dashboard (EPA)	DTXSID7041752
InChI InChI=1S/C10H12O4/c1-9-5-3-4-6(13-5)10(9,2)8(12)14-7(9)11/h5-6H,3-4H2,1-2H3/t5-,6+,9-,10+ Y Key: DHZBEENLJMYSHQ-YUMGAWCOSA-N Y
SMILES O=C2OC([C@@]1(C)[C@@H]3CC[C@@H](O3)[C@]12C)=O
Properties
Chemical formula	C10H12O4
Molar mass	196.202 g·mol−1
Density	1.41 g/cm3
Melting point	212 °C (414 °F; 485 K)
Pharmacology
ATC code	None
Routes of administration	Topical
Legal status	NZ: Class A US: ℞-only [1]
Hazards
Occupational safety and health (OHS/OSH):
Main hazards	Highly toxic
GHS labelling:
Pictograms
Signal word	Danger
Hazard statements	H300, H315, H319, H335
Precautionary statements	P261, P264, P270, P271, P280, P301+P310, P302+P352, P304+P340, P305+P351+P338, P312, P321, P330, P332+P313, P337+P313, P362, P403+P233, P405, P501
NFPA 704 (fire diamond)	4 1 1
Lethal dose or concentration (LD, LC):
LD50 (median dose)	0.03–0.5 mg/kg (human)
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). N  verify  (what is  YN ?) Infobox references

Cantharidin

Clinical data
Trade names	Ycanth, others
License data	US  DailyMed:  Cantharidin
Routes of administration	Topical
Legal status
Legal status	US: ℞-only
Identifiers
DrugBank	DB12328
CompTox Dashboard (EPA)	DTXSID7041752
ECHA InfoCard	100.000.240
Chemical and physical data
Formula	C10H12O4
Molar mass	196.202 g·mol−1

Cantharidin is an odorless, colorless fatty substance of the terpenoid class, which is secreted by many species of blister beetles. ^[a] Its main current use in pharmacology is treating molluscum contagiosum and warts topically. ^[2] It is a burn agent and poisonous in large doses, and has been historically used as aphrodisiacs (Spanish fly). In its natural form, cantharidin is secreted by the male blister beetle, and given to the female as a copulatory gift during mating. Afterwards, the female beetle covers her eggs with it as a defense against predators.

Poisoning from cantharidin is a significant veterinary concern, especially in horses, but it can also be poisonous to humans if taken internally (where the source is usually experimental self-exposure). Externally, cantharidin is a potent vesicant (blistering agent), exposure to which can cause severe chemical burns. Properly dosed and applied, the same properties have also been used therapeutically, for instance, for treatment of skin conditions, such as molluscum contagiosum infection of the skin.

Cantharidin is classified as an extremely hazardous substance in the United States, and is subject to strict reporting requirements by facilities that produce, store, or use it in significant quantities. ^[3]

Chemistry

Structure and nomenclature

Cantharidin, from the Greek kantharis, for beetle, ^[4] is an odorless, colorless natural product with solubility in various organic solvents,^{[ specify ]} but only slight solubility in water. ^[5] Its skeleton is tricyclic, formally, a tricyclo-[5.2.1.0^2,6]decane skeleton. Its functionalities include a carboxylic acid anhydride (−CO−O−CO−) substructure in one of its rings, as well as a bridging ether in its bicyclic ring system.

Biosynthesis from farnesol ⁠— ⁠bonds to be formed and major atoms to be added are in blue; while bonds to be broken and atoms/structural segments to be removed are in red.

The complete mechanism of the biosynthesis of cantharidin is unknown. Its framework formally consists of two isoprene units. ^[6] However, feeding studies indicate that the biosynthetic process is more complicated, and not a simple product of geranyl pyrophosphate or related ten-carbon parent structure, as the seeming monoterpene nature would suggest. Instead, there is a farnesol (15-carbon) precursor from which certain carbon segments are later excised. ^[7]

Distribution and availability

The level of cantharidin in blister beetles can be quite variable. Among blister beetles of the genus Epicauta in Colorado, E. pennsylvanica contains about 0.2 mg, E. maculata contains 0.7 mg, and E. immaculata contains 4.8 mg per beetle; males also contain higher levels than females. ^[8]

Males of Berberomeloe majalis have higher level of cantharidin per beetle: 64.22 ± 51.28 mg/g (dry weight) and 9.10 ± 12.64 mg/g (d. w.). Cantharidin content in haemolymph is also higher in males (80.9 ± 106.5 μg/g) than in females (20.0 ± 41.5 μg/g). ^[9]

History

Lytta vesicatoria (a.k.a "Spanish fly"), a beetle that secretes cantharidin.

Aphrodisiac preparations

Preparations made from blister beetles (particularly "Spanish fly") have been used since ancient times as an aphrodisiac, possibly because their physical effects were perceived to mimic those of sexual arousal, ^[10] and because they can cause prolonged erection or priapism in men. ^[11] These preparations were known as cantharides, from the Greek word for "beetle".

Examples of such use found in historical sources include:

• The ancient Roman historian Tacitus relates that a cantharid preparation was used by the empress Livia, wife of Augustus Caesar, to entice members of the imperial family or dinner guests to commit sexual indiscretions (thus, providing her information to hold over them). ^[12]
• The German emperor Henry IV (1050–1106) is said to have consumed cantharides. ^[13]
• The French surgeon Ambroise Paré (1510–1590) described a case in 1572 of a man suffering from "the most frightful satyriasis" after taking a potion composed of nettles and a cantharid extract. ^[14] This is perhaps the same man of whom Paré relates that a courtesan sprinkled a cantharid powder on food she served to him, after which the man experienced "violent priapism" and anal bleeding, of which he later died. Paré also cites the case of a priest who died of hematuria after swallowing a dose of cantharides, which he intended to fortify his sex drive. ^[15]
• Cantharides were in widespread use among the upper classes in France in the 1600s, despite being a banned substance. Police searches in connection with a rash of poisonings around 1680 turned up many stashes of "bluish flies", which were known to be used in the preparation of aphrodisiac potions. ^[15]
• The French sorceress Catherine Monvoisin (known as "La Voisin," c. 1640–1680) was recorded in the 1670s as having prepared a love charm made from Spanish fly mixed with dried mole's blood and bat's blood. ^[16]
• Aphrodisiac sweets presumably laced with cantharides were circulated within libertine circles during the 1700s in France. They were multicolored tablets nicknamed "pastilles de Richelieu," after the Maréchal de Richelieu, a notorious libertine (not to be confused with his great-uncle, the Cardinal Richelieu) who procured sexual encounters for King Louis XV. ^{[15] [17]}
• The French writer Donatien Alphonse François ⁠— ⁠notoriously known as the Marquis de Sade (1740–1814) ⁠— ⁠is said to have given aniseed-flavored pastilles laced with Spanish fly to two prostitutes at a pair of orgies in 1772, poisoning and nearly killing them. He was sentenced to death for that (and for the crime of sodomy), but was later reprieved on appeal. ^{[18] [19]}

Non-aphrodisiac uses

• The Spanish clergyman Juan de Horozco y Covarrubias (es) (c. 1540–1610) reported the use of blister beetles as a poison as well as an aphrodisiac. ^[20]
• Preparations of dried blister beetles were at one time used as a treatment for smallpox. ^[21] As late as 1892, Andrew Taylor Still, the founder of osteopathy, recommended inhaling a tincture of cantharidin as an effective preventative and treatment for smallpox, decrying vaccination. ^[22]

Pharmaco-chemical isolation

Cantharidin was first isolated as a chemically pure substance in 1810 by Pierre Robiquet, ^[23] a French chemist then living in Paris. Robiquet isolated cantharidin as the active ingredient in pharmacological preparations of Lytta vesicatoria , a.k.a. "Spanish fly", a species of blister beetle. This was one of the first historical instances of the identification and extraction of a simple active principle from a complex medicine.

Robiquet found cantharidin to be an odorless and colorless solid at room temperature. He demonstrated that it was the active principle responsible for the aggressively blistering properties of the coating of the eggs of the blister beetle, and additionally established that cantharidin had toxic properties comparable in degree to those of the most virulent poisons known in the 19th century, such as strychnine. ^[24]

Other uses of the pharmacological isolate

• Diluted solutions of cantharidin can be used as a topical medication to remove warts ^{[25] [26]} and tattoos, and to treat the small papules of molluscum contagiosum . ^[27]
• In Santería rituals, cantharides are used in incense. ^[28]

Veterinary issues

Poisoning by Epicauta species from cantharidin is a significant veterinary concern, especially in horses; species infesting feedstocks depend on region—e.g., Epicauta pennsylvanica (black blister beetle) in the U.S. midwest; and E. occidentalis, temexia, and vittata species (striped blister beetles) in the U.S. southwest—where the concentrations of the agent in each can vary substantially. ^[5] Beetles feed on weeds, and occasionally move into crop fields used to produce livestock feeds (e.g., alfalfa), where they are found to cluster and find their way into baled hay, e.g., a single flake (4–5 in. section ^[29] ) may have several hundred insects, or none at all. ^[5] Horses are very sensitive to the cantharidin produced by beetle infestations: the LD₅₀ for horses is roughly 1 mg/kg of the horse's body weight. Horses may be accidentally poisoned when fed bales of fodder with blister beetles in them. ^[30]

Great bustards, a strongly polygynous bird species, ^[31] are not immune to the toxicity of cantharidin; they become intoxicated after ingesting blister beetles. However, cantharidin has activity also against parasites that infect them. ^{[32] [33]} Great bustards may eat toxic blister beetles of the genus Meloe to increase the sexual arousal of males. ^[34]

Human medical issues

General risks

As a blister agent, cantharidin has the potential to cause adverse effects when used medically; for this reason, it has been included in a list of "problem drugs" used by dermatologists and emergency personnel. ^[35] However, this references unregulated sources of cantharidin. ^[36] In July 2023, the US FDA approved a topical formulation of cantharidin (Ycanth) for the treatment of molluscum contagiosum. ^[37]

When ingested by humans, the LD₅₀ is unknown, but fatal doses have been recorded between 10 mg and 65 mg. ^[38] The median lethal dose appears to be around 1 mg/kg ^[39] but individuals have survived after consuming oral doses as high as 175 mg. ^[40] Ingesting cantharidin can initially cause severe damage to the lining of the gastrointestinal and urinary tracts, and may also cause permanent renal damage. Symptoms of cantharidin poisoning include blood in the urine, abdominal pain, and (rarely) prolonged erections. ^[36]

Risks of aphrodisiac use

Main article: Lytta vesicatoria

The extreme toxicity of cantharidin makes any use as an aphrodisiac highly dangerous. ^{[41] [42]} As a result, it is illegal to sell (or use) cantharidin or preparations containing it without a prescription in many countries. ^[35]

Research

Mechanism of action

Topical cantharidin is absorbed by the lipid membranes of epidermal cells, causing the release of serine proteases, enzymes that break the peptide bonds in proteins. This causes the disintegration of desmosomal plaques, cellular structures involved in cell-to-cell adhesion, leading to detachment of the tonofilaments that hold cells together. The process leads to the loss of cellular connections (acantholysis), and ultimately results in blistering of the skin. Lesions heal without scarring. ^{[43] [44]}

Pharmaceutical use

VP-102, an experimental drug-device combination that includes cantharidin delivered via a single-use applicator, was studied for the treatment of molluscum contagiosum, common warts, and genital warts. ^[45] . The efficacy of cantharidin was formally established for the treatment of molluscum in patients 2 years and older in two double-blind, randomized, placebo-controlled trials. It is now FDA-approved for the treatment of molluscum contagiousum under the brand name Ycanth and is marketed by Verrica Pharmaceuticals. ^[46]

Bioactivities

Cantharidin appears to have some effect in the topical treatment of cutaneous leishmaniasis in animal models. ^[47] In addition to topical medical applications, cantharidin and its analogues may have activity against cancer cells. ^{[48] [49] [50]} Laboratory studies with cultured tumor cells suggest that this activity may be the result of PP2A inhibition. ^{[51] [52]}

Notes

1. Including broadly in genus Epicauta , genus Berberomeloe , and in species Lytta vesicatoria (Spanish fly). False blister beetles, cardinal beetles, and soldier beetles also produce cantharidin.

References

1. "Ycanth- cantharidin solution". DailyMed. 25 July 2023. Archived from the original on 29 August 2023. Retrieved 28 August 2023.
2. "FDA approves first treatment for molluscum contagiosum". FDA. 24 July 2023.
3. As defined in Section 302 of the U.S. Emergency Planning and Community Right-to-Know Act (42 U.S.C. 11002). See "40 C.F.R.: Appendix A to Part 355—The List of Extremely Hazardous Substances and Their Threshold Planning Quantities" Archived 25 February 2012 at the Wayback Machine (PDF) (1 July 2008 ed.). U.S. Government Printing Office. Retrieved 29 October 2011.
4. A Dictionary of Entomology. CABI. 2011. p. 253.
5. Schmitz DG (2013). "Overview of Cantharidin Poisoning (Blister Beetle Poisoning)". In Aiello SE, Moses MA (eds.). The Merck Veterinary Manual. Kenilworth, NJ, USA: Merck Sharp & Dohme. ISBN   978-0911910612. Archived from the original on 22 December 2015. Retrieved 14 December 2015.
6. Petroski RJ, McCormick SP, eds. (2012). Secondary-Metabolite Biosynthesis and Metabolism. Springer Science & Business Media. ISBN   978-0-306-44309-1.
7. Jiang M, Lü S, Zhang Y (January 2017). "The Potential Organ Involved in Cantharidin Biosynthesis in Epicauta chinensis Laporte (Coleoptera: Meloidae)". Journal of Insect Science. 17 (2): 52. doi:10.1093/jisesa/iex021. PMC   5633858 . PMID   28423415.
8. Capinera JL, Gardner DR, Stermitz FR (1985). "Cantharidin Levels in Blister Beetles (Coleoptera: Meloidae) Associated with Alfalfa in Colorado". Journal of Economic Entomology. 78 (5): 1052–1055. doi:10.1093/jee/78.5.1052.
9. Bravo C, Mas-Peinado P, Bautista LM, Blanco G, Alonso JC, García-París M (2017). "Cantharidin is conserved across phylogeographic lineages and present in both morphs of Iberian Berberomeloe blister beetles (Coleoptera, Meloidae)". Zoological Journal of the Linnean Society. 180 (4): 790–804. doi:10.1093/zoolinnean/zlw016. hdl: 10261/153832 .
10. John L. Capinera, Encyclopedia of Entomology, Volume 4, Springer Science & Business Media, 2008. p.2010
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12. James P (1995). Ancient Inventions. Ballantine Books. p.  177. ISBN   978-0-345-40102-1.
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16. Richard Cavendish, The Black Arts (London: Pan Books, 1969), p. 333.
17. Jacques Levron, Le Maréchal de Richelieu, un libertin fastueux (Paris, Perrin, 1971).
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21. Closs JF (1767). A New Method of Curing the Small-pox ... with a Specimen of Miscellaneous Observations on Medical Subjects; from the Latin ... by a Physician. Hawes. Archived from the original on 14 January 2023. Retrieved 15 April 2017. Cantharides referred to throughout the book.
22. Andrew Taylor Still, The philosophy and mechanical principles of osteopathy, 1892, chapter 12: "Smallpox". The 1902 edition is available here Archived 14 July 2019 at the Wayback Machine .
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29. Rockett J, Bosted S (2015). Veterinary Clinical Procedures in Large Animal Practices. Boston, MA, USA: Cengage Learning. p. 65. ISBN   978-1305537651 . Retrieved 14 December 2015.
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31. Alonso JC, Magaña M, Palacín C, Martín CA (2010). "Correlates of male mating success in great bustard leks: the effects of age, weight, and display effort". Behavioral Ecology and Sociobiology. 64 (10): 1589–1600. Bibcode:2010BEcoS..64.1589A. doi:10.1007/s00265-010-0972-6. hdl:10261/76985. S2CID   8741416.
32. Bravo C, Bautista LM, García-París M, Blanco G, Alonso JC (2014). "Males of a strongly polygynous species consume more poisonous food than females". PLOS ONE. 9 (10): e111057. Bibcode:2014PLoSO...9k1057B. doi: 10.1371/journal.pone.0111057 . PMC   4206510 . PMID   25337911.
33. Sánchez-Barbudo IS, Camarero PR, García-Montijano M, Mateo R (January 2012). "Possible cantharidin poisoning of a great bustard (Otis tarda)". Toxicon. 59 (1): 100–103. Bibcode:2012Txcn...59..100S. doi:10.1016/j.toxicon.2011.10.002. hdl: 10261/143513 . PMID   22001622.
34. Heneberg P (2016). "On Otis tarda and Marquis de Sade: what motivates male Great Bustards to consume Blister Beetles (Meloidae)?". Journal of Ornithology. 57 (4): 1123–1125. Bibcode:2016JOrni.157.1123H. doi:10.1007/s10336-016-1369-8. S2CID   17325635.
35. Karras DJ, Farrell SE, Harrigan RA, Henretig FM, Gealt L (September 1996). "Poisoning from "Spanish fly" (cantharidin)". The American Journal of Emergency Medicine. 14 (5): 478–483. doi:10.1016/S0735-6757(96)90158-8. PMID   8765116. While most commonly available preparations of Spanish fly contain cantharidin in negligible amounts, if at all, the chemical is available illicitly in concentrations capable of causing severe toxicity. Symptoms of cantharidin poisoning include burning of the mouth, dysphagia, nausea, hematemesis, gross hematuria, and dysuria. Mucosal erosion and hemorrhage is seen in the upper gastrointestinal (GI) tract. Renal dysfunction is common and related to acute tubular necrosis and glomerular destruction.
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42. Sandroni P (October 2001). "Aphrodisiacs past and present: a historical review". Clinical Autonomic Research. 11 (5): 303–307. doi:10.1007/BF02332975. PMID   11758796. S2CID   32348540. Cantharidin ("Spanish fly") is a chemical with vesicant properties derived from blister beetles, which has been used for millennia as a sexual stimulant by both sexes. Its mode of action is by inhibition of phosphodiesterase and protein phosphatase activity and stimulation of β-receptors, inducing vascular congestion and inflammation. Morbidity from its abuse is significant. The gastrointestinal tract sustains the brunt of toxicity, resulting in fatal hemorrhages. Renal toxicity is a result of its renal excretion, which may lead to acute tubular necrosis. Cardiac effects are most likely due to hemorrhagic shock, but they also can be due to myofibril degeneration, mitochondrial swelling, and pericardial and subendocardial hemorrhages.
43. Moed L, Shwayder TA, Chang MW (October 2001). "Cantharidin revisited: a blistering defense of an ancient medicine". Archives of Dermatology. 137 (10): 1357–1360. doi: 10.1001/archderm.137.10.1357 . PMID   11594862.
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47. Ghaffarifar F (October 2010). "Leishmania major: in vitro and in vivo anti-leishmanial effect of cantharidin". Experimental Parasitology. 126 (2): 126–129. doi: 10.1016/j.exppara.2010.04.004 . PMID   20435039.
48. Ratcliffe NA, Mello CB, Garcia ES, Butt TM, Azambuja P (October 2011). "Insect natural products and processes: new treatments for human disease". Insect Biochemistry and Molecular Biology. 41 (10): 747–769. Bibcode:2011IBMB...41..747R. doi:10.1016/j.ibmb.2011.05.007. PMID   21658450.
49. Chen YN, Cheng CC, Chen JC, Tsauer W, Hsu SL (October 2003). "Norcantharidin-induced apoptosis is via the extracellular signal-regulated kinase and c-Jun-NH2-terminal kinase signaling pathways in human hepatoma HepG2 cells". British Journal of Pharmacology. 140 (3): 461–470. doi:10.1038/sj.bjp.0705461. PMC   1574052 . PMID   12970086.
50. Zhang C, Peng Y, Wang F, Tan X, Liu N, Fan S, et al. (December 2010). "A synthetic cantharidin analog for the enhancement of doxorubicin suppression of stem cell-derived aggressive sarcoma". Biomaterials. 31 (36): 9535–9543. doi:10.1016/j.biomaterials.2010.08.059. PMID   20875681.
51. Dorn DC, Kou CA, Png KJ, Moore MA (May 2009). "The effect of cantharidins on leukemic stem cells". International Journal of Cancer. 124 (9): 2186–2199. doi: 10.1002/ijc.24157 . PMID   19123473. S2CID   38088568.
52. Li W, Xie L, Chen Z, Zhu Y, Sun Y, Miao Y, et al. (May 2010). "Cantharidin, a potent and selective PP2A inhibitor, induces an oxidative stress-independent growth inhibition of pancreatic cancer cells through G2/M cell-cycle arrest and apoptosis". Cancer Science. 101 (5): 1226–1233. doi: 10.1111/j.1349-7006.2010.01523.x . PMC   11158714 . PMID   20331621. S2CID   24345174.

External links

• Cantharidin : origin and synthesis at Lycée Faidherbe de Lille