Sp1 transcription factor

SP1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1SP1, 1SP2, 1VA1, 1VA2, 1VA3
Identifiers
Aliases	SP1 , entrez:6667, Sp1 transcription factor
External IDs	OMIM: 189906 MGI: 98372 HomoloGene: 8276 GeneCards: SP1
Gene location (Human) Chr. Chromosome 12 (human) [1] Band 12q13.13 Start 53,380,176 bp [1] End 53,416,446 bp [1]
Gene location (Mouse) Chr. Chromosome 15 (mouse) [2] Band 15|15 F3 Start 102,314,578 bp [2] End 102,344,839 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in nipple cancellous bone monocyte vulva lactiferous duct cardia thymus cavity of mouth Achilles tendon internal globus pallidus Top expressed in vas deferens conjunctival fornix internal carotid artery hair follicle ciliary body lacrimal gland external carotid artery cornea left colon right lung lobe More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein homodimerization activity HMG box domain binding transcription factor binding histone deacetylase binding metal ion binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding bHLH transcription factor binding protein C-terminus binding protein binding histone acetyltransferase binding nucleic acid binding sequence-specific DNA binding RNA polymerase II transcription regulatory region sequence-specific DNA binding DNA binding double-stranded DNA binding DNA-binding transcription activator activity, RNA polymerase II-specific DNA-binding transcription factor activity RNA polymerase II cis-regulatory region sequence-specific DNA binding core promoter sequence-specific DNA binding DNA-binding transcription factor activity, RNA polymerase II-specific cis-regulatory region sequence-specific DNA binding Cellular component cytoplasm nucleoplasm protein-DNA complex nucleus transcription repressor complex Biological process positive regulation of hydrogen sulfide biosynthetic process rhythmic process regulation of transcription by RNA polymerase II positive regulation of transcription, DNA-templated viral process regulation of transcription, DNA-templated transcription, DNA-templated snRNA transcription by RNA polymerase II cellular response to insulin stimulus positive regulation of transcription by RNA polymerase II regulation of cholesterol biosynthetic process positive regulation of gene expression positive regulation of blood vessel endothelial cell migration positive regulation of angiogenesis positive regulation of vascular endothelial cell proliferation response to hydroperoxide Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 6667 20683 Ensembl ENSG00000185591 ENSMUSG00000001280 UniProt P08047 O89090 RefSeq (mRNA) NM_001251825 NM_003109 NM_138473 NM_013672 RefSeq (protein) NP_001238754 NP_003100 NP_612482 NP_038700 Location (UCSC) Chr 12: 53.38 – 53.42 Mb Chr 15: 102.31 – 102.34 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Transcription factor Sp1, also known as specificity protein 1* is a protein that in humans is encoded by the SP1 gene. ^[5]

Function

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, O-GlcNAcylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. ^[5]

In the SV40 virus, Sp1 binds to the GC boxes in the regulatory region (RR) of the genome.

Structure

SP1 belongs to the Sp/KLF family of transcription factors. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The SP1 transcription factor contains two glutamine-rich activation domains at its N-terminus that are believed to be necessary for promoter trans-activation. ^[6] SP1 most notably contains three zinc finger protein motifs at its C-terminus, by which it binds directly to DNA and allows for interaction of the protein with other transcriptional regulators. Its zinc fingers are of the Cys₂/His₂ type and bind the consensus sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box element). Some 12,000 SP-1 binding sites are found in the human genome. ^[7]

Applications

Sp1 has been used as a control protein to compare with when studying the increase or decrease of the aryl hydrocarbon receptor and/or the estrogen receptor, since it binds to both and generally remains at a relatively constant level. ^[8]

Recently, a putative promoter region in FTMT, and positive regulators {SP1, cAMP response element-binding protein (CREB), and Ying Yang 1 (YY1)] and negative regulators [GATA2, forkhead box protein A1 (FoxA1), and CCAAT enhancer-binding protein b (C/EBPb)] of FTMT transcription have been identified (Guaraldo et al, 2016).The effect of DFP on the DNA-binding activity of these regulators to the FTMT promoter was examined using chromatin immunoprecipitation (ChIP) assay. Among the regulators, only SP1 displayed significantly increased DNA- binding activity following DFP treatment in a dose-dependent manner. SP1 knockdown by siRNA abolished the DFP-induced increase in the mRNA levels of FTMT, indicating SP1-mediated regulation of FTMT expression in the presence of DFP. Treatment with Deferiprone increased the expression of cytoplasmic and nuclear SP1 with predominant localization in the nucleus. ^[9]

Inhibitors

Plicamycin, an antineoplastic antibiotic produced by Streptomyces plicatus , and Withaferin A, a steroidal lactone from Withania somnifera plant are known to inhibit Sp1 transcription factor. ^{[10] [11]}

miR-375-5p microRNA significantly decreased expression of SP1 and YAP1 in colorectal cancer cells. SP1 and YAP1 mRNAs are direct targets of miR-375-5p. ^[12]

Interactions

Sp1 transcription factor has been shown to interact with:

• AATF, ^[13]
• CEBPB, ^{[14] [15]}
• COL1A1, ^[16]
• E2F1, ^{[17] [18] [19]}
• FOSL1, ^[20]
• GABPA, ^[21]
• HDAC1, ^{[13] [22] [23] [24]}
• HDAC2, ^{[23] [24] [25]}
• HMGA1, ^[15]
• HCFC1, ^{[26] [27]}
• HTT, ^[28]
• KLF6, ^[29]
• MEF2C, ^[30]
• MEF2D, ^[31]
• MSX1, ^[32]
• Myogenin, ^[33]
• POU2F1, ^{[26] [34]}
• PPP1R13L, ^[35]
• PSMC5, ^{[36] [37]}
• PML, ^[38]
• RELA, ^{[39] [40]}
• SMAD3, ^{[41] [42]}
• SUMO1, ^[36]
• SF1, ^[43]
• TAL1, ^[44]
• UBC. ^[36]
• WRN, ^[45]
• DDX3X

References

1. GRCh38: Ensembl release 89: ENSG00000185591 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000001280 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: Sp1 transcription factor".
6. Li, Lin; He, Shihua; Sun, Jian-Min; Davie, James R (2004-08-01). "Gene regulation by Sp1 and Sp3". Biochemistry and Cell Biology. 82 (4): 460–471. doi:10.1139/o04-045. ISSN   0829-8211. PMID   15284899.
7. Zhang B, Song L, Cai J, Li L, Xu H, Li M, et al. (May 2019). "The LIM protein Ajuba/SP1 complex forms a feed forward loop to induce SP1 target genes and promote pancreatic cancer cell proliferation". Journal of Experimental & Clinical Cancer Research. 38 (1): 205. doi:10.1186/s13046-019-1203-2. PMC   6525466 . PMID   31101117.
8. Wormke M, Stoner M, Saville B, Walker K, Abdelrahim M, Burghardt R, Safe S (March 2003). "The aryl hydrocarbon receptor mediates degradation of estrogen receptor alpha through activation of proteasomes". Molecular and Cellular Biology. 23 (6): 1843–55. doi:10.1128/MCB.23.6.1843-1855.2003. PMC   149455 . PMID   12612060.
9. Hara Y, Yanatori I, Tanaka A, Kishi F, Lemasters JJ, Nishina S, et al. (November 2020). "Iron loss triggers mitophagy through induction of mitochondrial ferritin". EMBO Reports. 21 (11): e50202. doi:10.15252/embr.202050202. PMC   7645172 . PMID   32975364.
10. Choi ES, Nam JS, Jung JY, Cho NP, Cho SD (November 2014). "Modulation of specificity protein 1 by mithramycin A as a novel therapeutic strategy for cervical cancer". Scientific Reports. 4: 7162. Bibcode:2014NatSR...4E7162C. doi:10.1038/srep07162. PMC   4241519 . PMID   25418289.
11. Prasanna KS, Shilpa P, Salimath BP (2009). "Withaferin A suppresses the expression of vascular endothelial growth factor in Ehrlich ascites tumor cells via Sp1 transcription" (PDF). Current Trends in Biotechnology and Pharmacy. 3 (2): 138–148.^{[ permanent dead link ]}
12. Xu X, Chen X, Xu M, Liu X, Pan B, Qin J, et al. (September 2019). "miR-375-3p suppresses tumorigenesis and partially reverses chemoresistance by targeting YAP1 and SP1 in colorectal cancer cells". Aging. 11 (18): 7357–7385. doi:10.18632/aging.102214. PMC   6781994 . PMID   31543507.
13. Di Padova M, Bruno T, De Nicola F, Iezzi S, D'Angelo C, Gallo R, et al. (September 2003). "Che-1 arrests human colon carcinoma cell proliferation by displacing HDAC1 from the p21WAF1/CIP1 promoter". The Journal of Biological Chemistry. 278 (38): 36496–504. doi: 10.1074/jbc.M306694200 . PMID   12847090.
14. Liu YW, Tseng HP, Chen LC, Chen BK, Chang WC (July 2003). "Functional cooperation of simian virus 40 promoter factor 1 and CCAAT/enhancer-binding protein beta and delta in lipopolysaccharide-induced gene activation of IL-10 in mouse macrophages". Journal of Immunology. 171 (2): 821–8. doi: 10.4049/jimmunol.171.2.821 . PMID   12847250.
15. Foti D, Iuliano R, Chiefari E, Brunetti A (April 2003). "A nucleoprotein complex containing Sp1, C/EBP beta, and HMGI-Y controls human insulin receptor gene transcription". Molecular and Cellular Biology. 23 (8): 2720–32. doi:10.1128/MCB.23.8.2720-2732.2003. PMC   152545 . PMID   12665574.
16. Li L, Artlett CM, Jimenez SA, Hall DJ, Varga J (October 1995). "Positive regulation of human alpha 1 (I) collagen promoter activity by transcription factor Sp1". Gene. 164 (2): 229–34. doi:10.1016/0378-1119(95)00508-4. PMID   7590335.
17. Lin SY, Black AR, Kostic D, Pajovic S, Hoover CN, Azizkhan JC (April 1996). "Cell cycle-regulated association of E2F1 and Sp1 is related to their functional interaction". Molecular and Cellular Biology. 16 (4): 1668–75. doi:10.1128/mcb.16.4.1668. PMC   231153 . PMID   8657142.
18. Rotheneder H, Geymayer S, Haidweger E (November 1999). "Transcription factors of the Sp1 family: interaction with E2F and regulation of the murine thymidine kinase promoter". Journal of Molecular Biology. 293 (5): 1005–15. doi:10.1006/jmbi.1999.3213. PMID   10547281.
19. Karlseder J, Rotheneder H, Wintersberger E (April 1996). "Interaction of Sp1 with the growth- and cell cycle-regulated transcription factor E2F". Molecular and Cellular Biology. 16 (4): 1659–67. doi:10.1128/mcb.16.4.1659. PMC   231152 . PMID   8657141.
20. Evellin S, Galvagni F, Zippo A, Neri F, Orlandini M, Incarnato D, et al. (March 2013). "FOSL1 controls the assembly of endothelial cells into capillary tubes by direct repression of αv and β3 integrin transcription". Molecular and Cellular Biology. 33 (6): 1198–209. doi:10.1128/MCB.01054-12. PMC   3592019 . PMID   23319049.
21. Galvagni F, Capo S, Oliviero S (March 2001). "Sp1 and Sp3 physically interact and co-operate with GABP for the activation of the utrophin promoter". Journal of Molecular Biology. 306 (5): 985–96. doi:10.1006/jmbi.2000.4335. hdl: 2318/141203 . PMID   11237613. S2CID   29403871.
22. Singh J, Murata K, Itahana Y, Desprez PY (March 2002). "Constitutive expression of the Id-1 promoter in human metastatic breast cancer cells is linked with the loss of NF-1/Rb/HDAC-1 transcription repressor complex". Oncogene. 21 (12): 1812–22. doi: 10.1038/sj.onc.1205252 . PMID   11896613.
23. Zhang Y, Dufau ML (September 2002). "Silencing of transcription of the human luteinizing hormone receptor gene by histone deacetylase-mSin3A complex". The Journal of Biological Chemistry. 277 (36): 33431–8. doi: 10.1074/jbc.M204417200 . PMID   12091390.
24. Sun JM, Chen HY, Moniwa M, Litchfield DW, Seto E, Davie JR (September 2002). "The transcriptional repressor Sp3 is associated with CK2-phosphorylated histone deacetylase 2". The Journal of Biological Chemistry. 277 (39): 35783–6. doi: 10.1074/jbc.C200378200 . PMID   12176973.
25. Won J, Yim J, Kim TK (October 2002). "Sp1 and Sp3 recruit histone deacetylase to repress transcription of human telomerase reverse transcriptase (hTERT) promoter in normal human somatic cells". The Journal of Biological Chemistry. 277 (41): 38230–8. doi: 10.1074/jbc.M206064200 . PMID   12151407.
26. Gunther M, Laithier M, Brison O (July 2000). "A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening". Molecular and Cellular Biochemistry. 210 (1–2): 131–42. doi:10.1023/A:1007177623283. PMID   10976766. S2CID   1339642.
27. Wysocka J, Myers MP, Laherty CD, Eisenman RN, Herr W (April 2003). "Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1". Genes & Development. 17 (7): 896–911. doi:10.1101/gad.252103. PMC   196026 . PMID   12670868.
28. Li SH, Cheng AL, Zhou H, Lam S, Rao M, Li H, Li XJ (March 2002). "Interaction of Huntington disease protein with transcriptional activator Sp1". Molecular and Cellular Biology. 22 (5): 1277–87. doi:10.1128/MCB.22.5.1277-1287.2002. PMC   134707 . PMID   11839795.
29. Botella LM, Sánchez-Elsner T, Sanz-Rodriguez F, Kojima S, Shimada J, Guerrero-Esteo M, et al. (December 2002). "Transcriptional activation of endoglin and transforming growth factor-beta signaling components by cooperative interaction between Sp1 and KLF6: their potential role in the response to vascular injury". Blood. 100 (12): 4001–10. doi: 10.1182/blood.V100.12.4001 . PMID   12433697.
30. Krainc D, Bai G, Okamoto S, Carles M, Kusiak JW, Brent RN, Lipton SA (October 1998). "Synergistic activation of the N-methyl-D-aspartate receptor subunit 1 promoter by myocyte enhancer factor 2C and Sp1". The Journal of Biological Chemistry. 273 (40): 26218–24. doi: 10.1074/jbc.273.40.26218 . PMID   9748305.
31. Park SY, Shin HM, Han TH (September 2002). "Synergistic interaction of MEF2D and Sp1 in activation of the CD14 promoter". Molecular Immunology. 39 (1–2): 25–30. doi:10.1016/S0161-5890(02)00055-X. PMID   12213324.
32. Shetty S, Takahashi T, Matsui H, Ayengar R, Raghow R (May 1999). "Transcriptional autorepression of Msx1 gene is mediated by interactions of Msx1 protein with a multi-protein transcriptional complex containing TATA-binding protein, Sp1 and cAMP-response-element-binding protein-binding protein (CBP/p300)". The Biochemical Journal. 339 (3): 751–8. doi:10.1042/0264-6021:3390751. PMC   1220213 . PMID   10215616.
33. Biesiada E, Hamamori Y, Kedes L, Sartorelli V (April 1999). "Myogenic basic helix-loop-helix proteins and Sp1 interact as components of a multiprotein transcriptional complex required for activity of the human cardiac alpha-actin promoter". Molecular and Cellular Biology. 19 (4): 2577–84. doi:10.1128/mcb.19.4.2577. PMC   84050 . PMID   10082523.
34. Ström AC, Forsberg M, Lillhager P, Westin G (June 1996). "The transcription factors Sp1 and Oct-1 interact physically to regulate human U2 snRNA gene expression". Nucleic Acids Research. 24 (11): 1981–6. doi:10.1093/nar/24.11.1981. PMC   145891 . PMID   8668525.
35. Takada N, Sanda T, Okamoto H, Yang JP, Asamitsu K, Sarol L, et al. (August 2002). "RelA-associated inhibitor blocks transcription of human immunodeficiency virus type 1 by inhibiting NF-kappaB and Sp1 actions". Journal of Virology. 76 (16): 8019–30. doi:10.1128/JVI.76.16.8019-8030.2002. PMC   155123 . PMID   12134007.
36. Wang YT, Chuang JY, Shen MR, Yang WB, Chang WC, Hung JJ (July 2008). "Sumoylation of specificity protein 1 augments its degradation by changing the localization and increasing the specificity protein 1 proteolytic process". Journal of Molecular Biology. 380 (5): 869–85. doi:10.1016/j.jmb.2008.05.043. PMID   18572193.
37. Su K, Yang X, Roos MD, Paterson AJ, Kudlow JE (June 2000). "Human Sug1/p45 is involved in the proteasome-dependent degradation of Sp1". The Biochemical Journal. 348 (2): 281–9. doi:10.1042/0264-6021:3480281. PMC   1221064 . PMID   10816420.
38. Vallian S, Chin KV, Chang KS (December 1998). "The promyelocytic leukemia protein interacts with Sp1 and inhibits its transactivation of the epidermal growth factor receptor promoter". Molecular and Cellular Biology. 18 (12): 7147–56. doi:10.1128/mcb.18.12.7147. PMC   109296 . PMID   9819401.
39. Kuang PP, Berk JL, Rishikof DC, Foster JA, Humphries DE, Ricupero DA, Goldstein RH (July 2002). "NF-kappaB induced by IL-1beta inhibits elastin transcription and myofibroblast phenotype". American Journal of Physiology. Cell Physiology. 283 (1): C58-65. doi:10.1152/ajpcell.00314.2001. PMID   12055073. S2CID   15753719.
40. Sif S, Gilmore TD (November 1994). "Interaction of the v-Rel oncoprotein with cellular transcription factor Sp1". Journal of Virology. 68 (11): 7131–8. doi:10.1128/JVI.68.11.7131-7138.1994. PMC   237152 . PMID   7933095.
41. Botella LM, Sánchez-Elsner T, Rius C, Corbí A, Bernabéu C (September 2001). "Identification of a critical Sp1 site within the endoglin promoter and its involvement in the transforming growth factor-beta stimulation". The Journal of Biological Chemistry. 276 (37): 34486–94. doi: 10.1074/jbc.M011611200 . PMID   11432852.
42. Poncelet AC, Schnaper HW (March 2001). "Sp1 and Smad proteins cooperate to mediate transforming growth factor-beta 1-induced alpha 2(I) collagen expression in human glomerular mesangial cells". The Journal of Biological Chemistry. 276 (10): 6983–92. doi: 10.1074/jbc.M006442200 . PMID   11114293.
43. Sugawara T, Saito M, Fujimoto S (August 2000). "Sp1 and SF-1 interact and cooperate in the regulation of human steroidogenic acute regulatory protein gene expression". Endocrinology. 141 (8): 2895–903. doi: 10.1210/endo.141.8.7602 . PMID   10919277.
44. Lécuyer E, Herblot S, Saint-Denis M, Martin R, Begley CG, Porcher C, et al. (October 2002). "The SCL complex regulates c-kit expression in hematopoietic cells through functional interaction with Sp1". Blood. 100 (7): 2430–40. doi: 10.1182/blood-2002-02-0568 . PMID   12239153.
45. Yamabe Y, Shimamoto A, Goto M, Yokota J, Sugawara M, Furuichi Y (November 1998). "Sp1-mediated transcription of the Werner helicase gene is modulated by Rb and p53". Molecular and Cellular Biology. 18 (11): 6191–200. doi:10.1128/mcb.18.11.6191. PMC   109206 . PMID   9774636.

Further reading

• Dreier B, Beerli RR, Segal DJ, Flippin JD, Barbas CF (August 2001). "Development of zinc finger domains for recognition of the 5'-ANN-3' family of DNA sequences and their use in the construction of artificial transcription factors". The Journal of Biological Chemistry. 276 (31): 29466–78. doi: 10.1074/jbc.M102604200 . PMID   11340073.
• Tseng L, Gao J, Mazella J, Zhu HH, Lane B (September 1997). "Differentiation-dependent and cell-specific regulation of the hIGFBP-1 gene in human endometrium". Annals of the New York Academy of Sciences. 828 (1): 27–37. Bibcode:1997NYASA.828...27T. doi:10.1111/j.1749-6632.1997.tb48521.x. PMID   9329821. S2CID   1601677.
• Dyson N (August 1998). "The regulation of E2F by pRB-family proteins". Genes & Development. 12 (15): 2245–62. doi: 10.1101/gad.12.15.2245 . PMID   9694791.
• Zhang Y, Dufau ML (June 2003). "Dual mechanisms of regulation of transcription of luteinizing hormone receptor gene by nuclear orphan receptors and histone deacetylase complexes". The Journal of Steroid Biochemistry and Molecular Biology. 85 (2–5): 401–14. doi:10.1016/S0960-0760(03)00230-9. PMID   12943729. S2CID   28512341.
• Kino T, Pavlakis GN (April 2004). "Partner molecules of accessory protein Vpr of the human immunodeficiency virus type 1". DNA and Cell Biology. 23 (4): 193–205. doi:10.1089/104454904773819789. PMID   15142377.
• Seelamgari A, Maddukuri A, Berro R, de la Fuente C, Kehn K, Deng L, et al. (September 2004). "Role of viral regulatory and accessory proteins in HIV-1 replication". Frontiers in Bioscience. 9 (1–3): 2388–413. doi:10.2741/1403. PMID   15353294.
• Le Rouzic E, Benichou S (February 2005). "The Vpr protein from HIV-1: distinct roles along the viral life cycle". Retrovirology. 2: 11. doi:10.1186/1742-4690-2-11. PMC   554975 . PMID   15725353.
• Kamine J, Chinnadurai G (June 1992). "Synergistic activation of the human immunodeficiency virus type 1 promoter by the viral Tat protein and cellular transcription factor Sp1". Journal of Virology. 66 (6): 3932–6. doi:10.1128/JVI.66.6.3932-3936.1992. PMC   241184 . PMID   1583736.
• Szpirer J, Szpirer C, Riviere M, Levan G, Marynen P, Cassiman JJ, et al. (September 1991). "The Sp1 transcription factor gene (SP1) and the 1,25-dihydroxyvitamin D3 receptor gene (VDR) are colocalized on human chromosome arm 12q and rat chromosome 7". Genomics. 11 (1): 168–73. doi:10.1016/0888-7543(91)90114-T. PMID   1662663.
• Gumucio DL, Rood KL, Blanchard-McQuate KL, Gray TA, Saulino A, Collins FS (October 1991). "Interaction of Sp1 with the human gamma globin promoter: binding and transactivation of normal and mutant promoters". Blood. 78 (7): 1853–63. doi: 10.1182/blood.V78.7.1853.1853 . PMID   1912570.
• Kamine J, Subramanian T, Chinnadurai G (October 1991). "Sp1-dependent activation of a synthetic promoter by human immunodeficiency virus type 1 Tat protein". Proceedings of the National Academy of Sciences of the United States of America. 88 (19): 8510–4. Bibcode:1991PNAS...88.8510K. doi: 10.1073/pnas.88.19.8510 . PMC   52538 . PMID   1924310.
• Courey AJ, Holtzman DA, Jackson SP, Tjian R (December 1989). "Synergistic activation by the glutamine-rich domains of human transcription factor Sp1". Cell. 59 (5): 827–36. doi:10.1016/0092-8674(89)90606-5. PMID   2512012. S2CID   2910480.
• Harrich D, Garcia J, Wu F, Mitsuyasu R, Gonazalez J, Gaynor R (June 1989). "Role of SP1-binding domains in in vivo transcriptional regulation of the human immunodeficiency virus type 1 long terminal repeat". Journal of Virology. 63 (6): 2585–91. doi:10.1128/JVI.63.6.2585-2591.1989. PMC   250732 . PMID   2657100.
• Jackson SP, Tjian R (October 1988). "O-glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation". Cell. 55 (1): 125–33. doi:10.1016/0092-8674(88)90015-3. PMID   3139301. S2CID   42523965.
• Kadonaga JT, Carner KR, Masiarz FR, Tjian R (December 1987). "Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain". Cell. 51 (6): 1079–90. doi:10.1016/0092-8674(87)90594-0. PMID   3319186. S2CID   19383553.
• Zhang R, Min W, Sessa WC (June 1995). "Functional analysis of the human endothelial nitric oxide synthase promoter. Sp1 and GATA factors are necessary for basal transcription in endothelial cells". The Journal of Biological Chemistry. 270 (25): 15320–6. doi: 10.1074/jbc.270.25.15320 . PMID   7541039.
• Hagen G, Dennig J, Preiss A, Beato M, Suske G (October 1995). "Functional analyses of the transcription factor Sp4 reveal properties distinct from Sp1 and Sp3". The Journal of Biological Chemistry. 270 (42): 24989–94. doi: 10.1074/jbc.270.42.24989 . PMID   7559627.
• Datta PK, Raychaudhuri P, Bagchi S (October 1995). "Association of p107 with Sp1: genetically separable regions of p107 are involved in regulation of E2F- and Sp1-dependent transcription". Molecular and Cellular Biology. 15 (10): 5444–52. doi:10.1128/mcb.15.10.5444. PMC   230794 . PMID   7565695.
• Wang L, Mukherjee S, Jia F, Narayan O, Zhao LJ (October 1995). "Interaction of virion protein Vpr of human immunodeficiency virus type 1 with cellular transcription factor Sp1 and trans-activation of viral long terminal repeat". The Journal of Biological Chemistry. 270 (43): 25564–9. doi: 10.1074/jbc.270.43.25564 . PMID   7592727.
• Howcroft TK, Palmer LA, Brown J, Rellahan B, Kashanchi F, Brady JN, Singer DS (July 1995). "HIV Tat represses transcription through Sp1-like elements in the basal promoter". Immunity. 3 (1): 127–38. doi: 10.1016/1074-7613(95)90165-5 . PMID   7621073.
• Ferrari N, Desmarais D, Royal A (July 1995). "Transcriptional activation of the neuronal peripherin-encoding gene depends on a G + C-rich element that binds Sp1 in vitro and in vivo". Gene. 159 (2): 159–65. doi: 10.1016/0378-1119(95)00140-2 . PMID   7622044.
• Tan NY, Midgley VC, Kavurma MM, Santiago FS, Luo X, Peden R, et al. (February 2008). "Angiotensin II-inducible platelet-derived growth factor-D transcription requires specific Ser/Thr residues in the second zinc finger region of Sp1". Circulation Research. 102 (4): e38-51. doi: 10.1161/CIRCRESAHA.107.167395 . PMID   18258854.

External links

• Sp1+Transcription+Factor at the US National Library of Medicine Medical Subject Headings (MeSH)
• FactorBook SP1

This article incorporates text from the United States National Library of Medicine, which is in the public domain.